The Influence of Adjuvant Type on the Immunogenicity of RBD/N
Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2
Virus.

J 2023

The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.

BRZUSKA, Gabriela; Marta ZIMNA; Klaudia BARANSKA; Boguslaw SZEWCZYK; Petra STRAKOVA et al.

Základní údaje

Originální název

The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.

Autoři

BRZUSKA, Gabriela; Marta ZIMNA; Klaudia BARANSKA; Boguslaw SZEWCZYK; Petra STRAKOVA; Daniel RŮŽEK a Ewelina KROL

Vydání

Microbiology Spectrum, American Society for Microbiology, 2023, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131191

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

SARS-CoV-2; adjuvants; antigen specificity; vaccines

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 1. 2024 15:40, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The emerging virus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2 virus), agent of COVID-19, appeared in December 2019 in Wuhan, China, and became a serious threat to global health and public safety. Many COVID-19 vaccines have been approved and licensed around the world. Most of the developed vaccines include S protein and induce an antibody-based immune response. Additionally, T-cell response to the SARS-CoV-2 antigens could be beneficial for combating the infection. The type of immune response is greatly dependent not only on the antigen, but also on adjuvants used in vaccine formulation. Here, we compared the effect of four different adjuvants (AddaS03, Alhydrogel/MPLA, Alhydrogel/ODN2395, Quil A) on the immunogenicity of a mixture of recombinant RBD and N SARS-CoV-2 proteins. We have analyzed the antibody and T-cell response specific to RBD and N proteins and assessed the impact of adjuvants on virus neutralization. Our results clearly indicated that Alhydrogel/MPLA and Alhydrogel/ODN2395 adjuvants elicited the higher titers of specific and cross-reactive antibodies to S protein variants from various SARS-CoV-2 and SARS-CoV-1 strains. Moreover, Alhydrogel/ODN2395 stimulated high cellular response to both antigens, as assessed by IFN-γ production. Importantly, sera collected from mice immunized with RBD/N cocktail in combination with these adjuvants exhibited neutralizing activity against the authentic SARS-CoV-2 virus as well as particles pseudotyped with S protein from various virus variants. The results from our study demonstrate the immunogenic potential of RBD and N antigens and point out the importance of adjuvants selection in vaccine formulation in order to enhance the immunological response.

Citovat

BRZUSKA, Gabriela; Marta ZIMNA; Klaudia BARANSKA; Boguslaw SZEWCZYK; Petra STRAKOVA; Daniel RŮŽEK a Ewelina KROL. The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus. Microbiology Spectrum. American Society for Microbiology, 2023, roč. 11, č. 3, s. 1-14. ISSN 2165-0497. Dostupné z: https://doi.org/10.1128/spectrum.02564-22.

@article{2296942,
   author = {Brzuska, Gabriela and Zimna, Marta and Baranska, Klaudia and Szewczyk, Boguslaw and Strakova, Petra and Růžek, Daniel and Krol, Ewelina},
   article_number = {3},
   doi = {https://doi.org/10.1128/spectrum.02564-22},
   keywords = {SARS-CoV-2; adjuvants; antigen specificity; vaccines},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.},
   url = {https://doi.org/10.1128/spectrum.02564-22},
   volume = {11},
   year = {2023}
}

TY  - JOUR
ID  - 2296942
AU  - Brzuska, Gabriela - Zimna, Marta - Baranska, Klaudia - Szewczyk, Boguslaw - Strakova, Petra - Růžek, Daniel - Krol, Ewelina
PY  - 2023
TI  - The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus.
JF  - Microbiology Spectrum
VL  - 11
IS  - 3
SP  - 1-14
EP  - 1-14
PB  - American Society for Microbiology
SN  - 21650497
KW  - SARS-CoV-2
KW  - adjuvants
KW  - antigen specificity
KW  - vaccines
UR  - https://doi.org/10.1128/spectrum.02564-22
N2  - The emerging virus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2 virus), agent of COVID-19, appeared in December 2019 in Wuhan, China, and became a serious threat to global health and public safety. Many COVID-19 vaccines have been approved and licensed around the world. Most of the developed vaccines include S protein and induce an antibody-based immune response. Additionally, T-cell response to the SARS-CoV-2 antigens could be beneficial for combating the infection. The type of immune response is greatly dependent not only on the antigen, but also on adjuvants used in vaccine formulation. Here, we compared the effect of four different adjuvants (AddaS03, Alhydrogel/MPLA, Alhydrogel/ODN2395, Quil A) on the immunogenicity of a mixture of recombinant RBD and N SARS-CoV-2 proteins. We have analyzed the antibody and T-cell response specific to RBD and N proteins and assessed the impact of adjuvants on virus neutralization. Our results clearly indicated that Alhydrogel/MPLA and Alhydrogel/ODN2395 adjuvants elicited the higher titers of specific and cross-reactive antibodies to S protein variants from various SARS-CoV-2 and SARS-CoV-1 strains. Moreover, Alhydrogel/ODN2395 stimulated high cellular response to both antigens, as assessed by IFN-γ production. Importantly, sera collected from mice immunized with RBD/N cocktail in combination with these adjuvants exhibited neutralizing activity against the authentic SARS-CoV-2 virus as well as particles pseudotyped with S protein from various virus variants. The results from our study demonstrate the immunogenic potential of RBD and N antigens and point out the importance of adjuvants selection in vaccine formulation in order to enhance the immunological response.
ER  -

BRZUSKA, Gabriela; Marta ZIMNA; Klaudia BARANSKA; Boguslaw SZEWCZYK; Petra STRAKOVA; Daniel RŮŽEK a Ewelina KROL. The Influence of Adjuvant Type on the Immunogenicity of RBD/N Cocktail Antigens as a Vaccine Candidate against SARS-CoV-2 Virus. \textit{Microbiology Spectrum}. American Society for Microbiology, 2023, roč.~11, č.~3, s.~1-14. ISSN~2165-0497. Dostupné z: https://doi.org/10.1128/spectrum.02564-22.

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