| BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. International Journal of Pharmaceutics. Elsevier B.V., 2023, roč. 643, August, s. 1-12. ISSN 0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211. |
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@article{2297377,
author = {Behera, Susrisweta and Veselý, Lukáš and Štůsek, Radim and Hauptmann, Astrid and Loerting, Thomas and Heger, Dominik},
article_number = {August},
doi = {http://dx.doi.org/10.1016/j.ijpharm.2023.123211},
keywords = {Stabilization; Freeze-concentrated solution; Hammett acidity function; Differential Scanning Calorimetry; Glass Transition Temperature; Cryomicroscopy; Sulfonephthalein indicators},
language = {eng},
issn = {0378-5173},
journal = {International Journal of Pharmaceutics},
title = {Investigating freezing-induced acidity changes in citrate buffers},
url = {https://doi.org/10.1016/j.ijpharm.2023.123211},
volume = {643},
year = {2023}
}
TY - JOUR
ID - 2297377
AU - Behera, Susrisweta - Veselý, Lukáš - Štůsek, Radim - Hauptmann, Astrid - Loerting, Thomas - Heger, Dominik
PY - 2023
TI - Investigating freezing-induced acidity changes in citrate buffers
JF - International Journal of Pharmaceutics
VL - 643
IS - August
SP - 1-12
EP - 1-12
PB - Elsevier B.V.
SN - 03785173
KW - Stabilization
KW - Freeze-concentrated solution
KW - Hammett acidity function
KW - Differential Scanning Calorimetry
KW - Glass Transition Temperature
KW - Cryomicroscopy
KW - Sulfonephthalein indicators
UR - https://doi.org/10.1016/j.ijpharm.2023.123211
N2 - Citrate buffers are commonly utilized in the field of biomolecule stabilization. We investigate their applicability in the frozen state within a range of initial pHs (2.5 to 8.0) and concentrations (0.02 to 0.60 M). Citrate buffer solutions subjected to various cooling and heating temperatures are examined in terms of the freezing-induced acidity changes, revealing that citrate buffers acidify upon cooling. The acidity is assessed with sulfonephthalein molecular probes frozen in the samples. Optical cryomicroscopy combined with differential scanning calorimetry was employed to investigate the causes of the observed acidity changes. The buffers partly crystallize and partly vitrify in the ice matrix; these processes influence the resulting pH and allow designing the optimal storage temperatures in the frozen state. The freezing-induced acidification apparently depends on the buffer concentration; at each pH, we suggest pertinent concentration, at which freezing causes minimal acidification.
ER -
BEHERA, Susrisweta, Lukáš VESELÝ, Radim ŠTŮSEK, Astrid HAUPTMANN, Thomas LOERTING a Dominik HEGER. Investigating freezing-induced acidity changes in citrate buffers. \textit{International Journal of Pharmaceutics}. Elsevier B.V., 2023, roč.~643, August, s.~1-12. ISSN~0378-5173. Dostupné z: https://dx.doi.org/10.1016/j.ijpharm.2023.123211.
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