DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

C 2020

DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

KOCURKOVÁ, Anna a Lukáš KUBALA

Základní údaje

Originální název

DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

Autoři

KOCURKOVÁ, Anna a Lukáš KUBALA

Vydání

Prague, CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS, od s. 134-138, 5 s. 1, 2020

Nakladatel

AMCA, spol. s r.o.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-88214-18-2

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 8. 2023 09:16, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Phagocytes play a key role in inflammation, a complex process underlying the pathogenesis of numerous chronic and acute diseases. Determination of phagocyte activation provides an essential information about inflammatory processes. Among strategies of how to estimate phagocyte activation status is determination of surface expression of selected receptors and adhesion molecules reflecting degranulation of phagocytes. Herein, the activation of mouse blood neutrophil granulocytes was analyzed in mice treated by schizophyllan (SPG), a (1-3)-beta-D-glucan produced as a cell wall constituent of fungi or plants with suggested immunomodulatory potential. The activation of neutrophil granulocytes was analyzed 2, 4, 6 and 8 hours after SPG i.v. application. Staining of cells with antibodies against Ly6G, CDllb and CD62l was performed in whole blood with consequent fixation and lysation of erythrocytes. Neutrophil granulocytes were identified based on typical forward and side scatter characteristics and expression of Ly6G surface antigen. Further, pro-inflammatory cytokine, TNF-alpha, was analyzed in plasma by ELISA. Application of SPG significantly increased expression of CDllb after 4 hours. CD62l was significantly decreased at 4 and 6 hours after SPG application. Overall, it can be concluded that SPG induced activation of neutrophil granulocytes in mice after intravenous application in time frame from 2 to 8 hours with the highest peak after 4 hours and that CDllb and CD621 are sensitive markers that can be applied for determination of activation of this abundant phagocyte population.

Návaznosti

EF16_025/0007381, projekt VaV

Název: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

Citovat

KOCURKOVÁ, Anna a Lukáš KUBALA. DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD. In Kalina Tomáš, Zuna Jan. CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS. Prague: AMCA, spol. s r.o., 2020, s. 134-138. 1. ISBN 978-80-88214-18-2.

@inbook{2301277,
   author = {Kocurková, Anna and Kubala, Lukáš},
   address = {Prague},
   booktitle = {CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS},
   editor = {Kalina Tomáš, Zuna Jan},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Prague},
   isbn = {978-80-88214-18-2},
   pages = {134-138},
   publisher = {AMCA, spol. s r.o.},
   title = {DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD},
   year = {2020}
}

TY  - CHAP
ID  - 2301277
AU  - Kocurková, Anna - Kubala, Lukáš
PY  - 2020
TI  - DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD
VL  - 1
PB  - AMCA, spol. s r.o.
CY  - Prague
SN  - 9788088214182
N2  - Phagocytes play a key role in inflammation, a complex process underlying the pathogenesis of numerous chronic and acute diseases. Determination of phagocyte activation provides an essential information about inflammatory processes. Among strategies of how to estimate phagocyte activation status is determination of surface expression of selected receptors and adhesion molecules reflecting degranulation of phagocytes. Herein, the activation of mouse blood neutrophil granulocytes was analyzed in mice treated by schizophyllan (SPG), a (1-3)-beta-D-glucan produced as a cell wall constituent of fungi or plants with suggested immunomodulatory potential. The activation of neutrophil granulocytes was analyzed 2, 4, 6 and 8 hours after SPG i.v. application. Staining of cells with antibodies against Ly6G, CDllb and CD62l was performed in whole blood with consequent fixation and lysation of erythrocytes. Neutrophil granulocytes were identified based on typical forward and side scatter characteristics and expression of Ly6G surface antigen. Further, pro-inflammatory cytokine, TNF-alpha, was analyzed in plasma by ELISA. Application of SPG significantly increased expression of CDllb after 4 hours. CD62l was significantly decreased at 4 and 6 hours after SPG application. Overall, it can be concluded that SPG induced activation of neutrophil granulocytes in mice after intravenous application in time frame from 2 to 8 hours with the highest peak after 4 hours and that CDllb and CD621 are sensitive markers that can be applied for determination of activation of this abundant phagocyte population.
ER  -

KOCURKOVÁ, Anna a Lukáš KUBALA. DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD. In Kalina Tomáš, Zuna Jan. \textit{CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS}. Prague: AMCA, spol. s r.o., 2020, s.~134-138. 1. ISBN~978-80-88214-18-2.

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