DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

C 2020

DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

KOCURKOVÁ, Anna and Lukáš KUBALA

Basic information

Original name

DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD

Authors

KOCURKOVÁ, Anna and Lukáš KUBALA

Edition

Prague, CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS, p. 134-138, 5 pp. 1, 2020

Publisher

AMCA, spol. s r.o.

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

Organization unit

Faculty of Science

ISBN

978-80-88214-18-2

Abstract

V originále

Phagocytes play a key role in inflammation, a complex process underlying the pathogenesis of numerous chronic and acute diseases. Determination of phagocyte activation provides an essential information about inflammatory processes. Among strategies of how to estimate phagocyte activation status is determination of surface expression of selected receptors and adhesion molecules reflecting degranulation of phagocytes. Herein, the activation of mouse blood neutrophil granulocytes was analyzed in mice treated by schizophyllan (SPG), a (1-3)-beta-D-glucan produced as a cell wall constituent of fungi or plants with suggested immunomodulatory potential. The activation of neutrophil granulocytes was analyzed 2, 4, 6 and 8 hours after SPG i.v. application. Staining of cells with antibodies against Ly6G, CDllb and CD62l was performed in whole blood with consequent fixation and lysation of erythrocytes. Neutrophil granulocytes were identified based on typical forward and side scatter characteristics and expression of Ly6G surface antigen. Further, pro-inflammatory cytokine, TNF-alpha, was analyzed in plasma by ELISA. Application of SPG significantly increased expression of CDllb after 4 hours. CD62l was significantly decreased at 4 and 6 hours after SPG application. Overall, it can be concluded that SPG induced activation of neutrophil granulocytes in mice after intravenous application in time frame from 2 to 8 hours with the highest peak after 4 hours and that CDllb and CD621 are sensitive markers that can be applied for determination of activation of this abundant phagocyte population.

Links

EF16_025/0007381, research and development project

Name: Preklinická progrese nových organických sloučenin s cílenou biologickou aktivitou

Citovat

KOCURKOVÁ, Anna and Lukáš KUBALA. DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD. In Kalina Tomáš, Zuna Jan. CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS. Prague: AMCA, spol. s r.o., 2020, p. 134-138. 1. ISBN 978-80-88214-18-2.

@inbook{2301277,
   author = {Kocurková, Anna and Kubala, Lukáš},
   address = {Prague},
   booktitle = {CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS},
   editor = {Kalina Tomáš, Zuna Jan},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Prague},
   isbn = {978-80-88214-18-2},
   pages = {134-138},
   publisher = {AMCA, spol. s r.o.},
   title = {DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD},
   year = {2020}
}

TY  - CHAP
ID  - 2301277
AU  - Kocurková, Anna - Kubala, Lukáš
PY  - 2020
TI  - DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD
VL  - 1
PB  - AMCA, spol. s r.o.
CY  - Prague
SN  - 9788088214182
N2  - Phagocytes play a key role in inflammation, a complex process underlying the pathogenesis of numerous chronic and acute diseases. Determination of phagocyte activation provides an essential information about inflammatory processes. Among strategies of how to estimate phagocyte activation status is determination of surface expression of selected receptors and adhesion molecules reflecting degranulation of phagocytes. Herein, the activation of mouse blood neutrophil granulocytes was analyzed in mice treated by schizophyllan (SPG), a (1-3)-beta-D-glucan produced as a cell wall constituent of fungi or plants with suggested immunomodulatory potential. The activation of neutrophil granulocytes was analyzed 2, 4, 6 and 8 hours after SPG i.v. application. Staining of cells with antibodies against Ly6G, CDllb and CD62l was performed in whole blood with consequent fixation and lysation of erythrocytes. Neutrophil granulocytes were identified based on typical forward and side scatter characteristics and expression of Ly6G surface antigen. Further, pro-inflammatory cytokine, TNF-alpha, was analyzed in plasma by ELISA. Application of SPG significantly increased expression of CDllb after 4 hours. CD62l was significantly decreased at 4 and 6 hours after SPG application. Overall, it can be concluded that SPG induced activation of neutrophil granulocytes in mice after intravenous application in time frame from 2 to 8 hours with the highest peak after 4 hours and that CDllb and CD621 are sensitive markers that can be applied for determination of activation of this abundant phagocyte population.
ER  -

KOCURKOVÁ, Anna and Lukáš KUBALA. DETERMINATION OF ACTIVATION OF PHAGOCYTES IN MOUSE BLOOD. In Kalina Tomáš, Zuna Jan. \textit{CURRENT INVESTIGATIONS IN BLOOD AND CANCER CELLS}. Prague: AMCA, spol. s r.o., 2020, p.~134-138. 1. ISBN~978-80-88214-18-2.

Detailed Information on Publication Record