Capillary Electrophoresis with Interchangeable Cartridges for
Versatile and Automated Analyses of Dried Blood Spot Samples

J 2023

Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples

DVOŘÁK, Miloš; Ondrej MORAVČÍK a Pavel KUBÁŇ

Základní údaje

Originální název

Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples

Autoři

DVOŘÁK, Miloš; Ondrej MORAVČÍK a Pavel KUBÁŇ

Vydání

Analytical chemistry, American Chemical Society, 2023, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131500

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Anatomy; Calibration; Computer simulations; Genetics; Solvents

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 8. 2023 11:54, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

A novel concept forhighly versatile automated analyses of driedblood spot (DBS) samples by commercial capillary electrophoresis (CE)is presented. Two interchangeable CE cartridges with different fused-silicacapillaries were used for the DBS elutions and the DBS eluate analyses,respectively. The application of one CE cartridge with a wide-borecapillary reduced DBS processing times to a minimum (1-2 minper sample) while fitting the other CE cartridge with a narrow-borecapillary served for highly efficient CE analyses. A comprehensiveinvestigation of major variables affecting liquid handling in CE (capillarylength, internal diameter, and temperature) was carried out with theaim of optimizing both procedures and enabling their maximum flexibility.The application of two CE cartridges also enabled the employment ofCE detectors with different instrumental set-ups and/or principlesas was demonstrated by the optical detection of nonsteroidal anti-inflammatorydrugs (NSAIDs) and the conductivity detection of amino acids (AAs).The presented methods were optimized for the automated CE analysesof 36 DBS samples formed by a volumetric collection of 5 & mu;Lof capillary blood onto Whatman 903 discs and processed by directin-vial elution using the CE instrument. The precision of liquid transfersfor the automated DBS elutions was better than 0.9% and the precisionof CE analyses did not exceed 5.1 and 12.3% for the determinationof NSAIDs and AAs, respectively. Both methods were linear (R (2) & GE; 0.996) over the therapeutic (NSAIDs)and the endogenous (AAs) concentration ranges, had limits of quantificationbelow the typical analyte concentrations in human blood, and achievedsample throughputs of more than 6 DBSs per hour.

Citovat

DVOŘÁK, Miloš; Ondrej MORAVČÍK a Pavel KUBÁŇ. Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples. Analytical chemistry. American Chemical Society, 2023, roč. 95, č. 31, s. 11823-11830. ISSN 0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.3c02474.

@article{2304040,
   author = {Dvořák, Miloš and Moravčík, Ondrej and Kubáň, Pavel},
   article_number = {31},
   doi = {https://doi.org/10.1021/acs.analchem.3c02474},
   keywords = {Anatomy; Calibration; Computer simulations; Genetics; Solvents},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples},
   url = {https://doi.org/10.1021/acs.analchem.3c02474},
   volume = {95},
   year = {2023}
}

TY  - JOUR
ID  - 2304040
AU  - Dvořák, Miloš - Moravčík, Ondrej - Kubáň, Pavel
PY  - 2023
TI  - Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples
JF  - Analytical chemistry
VL  - 95
IS  - 31
SP  - 11823-11830
EP  - 11823-11830
PB  - American Chemical Society
SN  - 00032700
KW  - Anatomy
KW  - Calibration
KW  - Computer simulations
KW  - Genetics
KW  - Solvents
UR  - https://doi.org/10.1021/acs.analchem.3c02474
N2  - A novel concept forhighly versatile automated analyses of driedblood spot (DBS) samples by commercial capillary electrophoresis (CE)is presented. Two interchangeable CE cartridges with different fused-silicacapillaries were used for the DBS elutions and the DBS eluate analyses,respectively. The application of one CE cartridge with a wide-borecapillary reduced DBS processing times to a minimum (1-2 minper sample) while fitting the other CE cartridge with a narrow-borecapillary served for highly efficient CE analyses. A comprehensiveinvestigation of major variables affecting liquid handling in CE (capillarylength, internal diameter, and temperature) was carried out with theaim of optimizing both procedures and enabling their maximum flexibility.The application of two CE cartridges also enabled the employment ofCE detectors with different instrumental set-ups and/or principlesas was demonstrated by the optical detection of nonsteroidal anti-inflammatorydrugs (NSAIDs) and the conductivity detection of amino acids (AAs).The presented methods were optimized for the automated CE analysesof 36 DBS samples formed by a volumetric collection of 5 & mu;Lof capillary blood onto Whatman 903 discs and processed by directin-vial elution using the CE instrument. The precision of liquid transfersfor the automated DBS elutions was better than 0.9% and the precisionof CE analyses did not exceed 5.1 and 12.3% for the determinationof NSAIDs and AAs, respectively. Both methods were linear (R (2) & GE; 0.996) over the therapeutic (NSAIDs)and the endogenous (AAs) concentration ranges, had limits of quantificationbelow the typical analyte concentrations in human blood, and achievedsample throughputs of more than 6 DBSs per hour.
ER  -

DVOŘÁK, Miloš; Ondrej MORAVČÍK a Pavel KUBÁŇ. Capillary Electrophoresis with Interchangeable Cartridges for Versatile and Automated Analyses of Dried Blood Spot Samples. \textit{Analytical chemistry}. American Chemical Society, 2023, roč.~95, č.~31, s.~11823-11830. ISSN~0003-2700. Dostupné z: https://doi.org/10.1021/acs.analchem.3c02474.

Přehled o publikaci