NADH and NADPH peroxidases as antioxidant defense mechanisms in
intestinal sulfate-reducing bacteria

J 2023

NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria

KUSHKEVYCH, Ivan; Dani DORDEVIĆ; Mohammad I. ALBERFKANI; Márió GAJDÁCS; Eszter OSTORHÁZI et al.

Základní údaje

Originální název

NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria

Autoři

KUSHKEVYCH, Ivan; Dani DORDEVIĆ; Mohammad I. ALBERFKANI; Márió GAJDÁCS; Eszter OSTORHÁZI; Monika VÍTĚZOVÁ a Simon K.-M. R. RITTMANN

Vydání

Scientific Reports, Nature Research, 2023, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131515

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Biochemistry; Enzymes; Microbiology

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 2. 2024 17:19, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Animal and human feces typically include intestinal sulfate-reducing bacteria (SRB). Hydrogen sulfide and acetate are the end products of their dissimilatory sulfate reduction and may create a synergistic effect. Here, we report NADH and NADPH peroxidase activities from intestinal SRB Desulfomicrobium orale and Desulfovibrio piger. We sought to compare enzymatic activities under the influence of various temperature and pH regimes, as well as to carry out kinetic analyses of enzymatic reaction rates, maximum amounts of the reaction product, reaction times, maximum rates of the enzyme reactions, and Michaelis constants in cell-free extracts of intestinal SRB, D. piger Vib-7, and D. orale Rod-9, collected from exponential and stationary growth phases. The optimal temperature (35 °C) and pH (7.0) for both enzyme’s activity were determined. The difference in trends of Michaelis constants (Km) during exponential and stationary phases are noticeable between D. piger Vib-7 and D. orale Rod-9; D. orale Rod-9 showed much higher Km (the exception is NADH peroxidase of D. piger Vib-7: 1.42 ± 0.11 mM) during the both monitored phases. Studies of the NADH and NADPH peroxidases—as putative antioxidant defense systems of intestinal SRB and detailed data on the kinetic properties of this enzyme, as expressed by the decomposition of hydrogen peroxide—could be important for clarifying evolutionary mechanisms of antioxidant defense systems, their etiological role in the process of dissimilatory sulfate reduction, and their possible role in the development of bowel diseases.

Návaznosti

MUNI/A/1280/2022, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 3

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 3

MUNI/A/1502/2023, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 4

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 4

Citovat

KUSHKEVYCH, Ivan; Dani DORDEVIĆ; Mohammad I. ALBERFKANI; Márió GAJDÁCS; Eszter OSTORHÁZI; Monika VÍTĚZOVÁ a Simon K.-M. R. RITTMANN. NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria. Scientific Reports. Nature Research, 2023, roč. 13, č. 1, s. 1-11. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-023-41185-3.

@article{2304557,
   author = {Kushkevych, Ivan and Dordević, Dani and Alberfkani, Mohammad I. and Gajdács, Márió and Ostorházi, Eszter and Vítězová, Monika and Rittmann, Simon K.andM. R.},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-023-41185-3},
   keywords = {Biochemistry; Enzymes; Microbiology},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria},
   url = {https://doi.org/10.1038/s41598-023-41185-3},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2304557
AU  - Kushkevych, Ivan - Dordević, Dani - Alberfkani, Mohammad I. - Gajdács, Márió - Ostorházi, Eszter - Vítězová, Monika - Rittmann, Simon K.-M. R.
PY  - 2023
TI  - NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria
JF  - Scientific Reports
VL  - 13
IS  - 1
SP  - 1-11
EP  - 1-11
PB  - Nature Research
SN  - 20452322
KW  - Biochemistry
KW  - Enzymes
KW  - Microbiology
UR  - https://doi.org/10.1038/s41598-023-41185-3
N2  - Animal and human feces typically include intestinal sulfate-reducing bacteria (SRB). Hydrogen sulfide and acetate are the end products of their dissimilatory sulfate reduction and may create a synergistic effect. Here, we report NADH and NADPH peroxidase activities from intestinal SRB Desulfomicrobium orale and Desulfovibrio piger. We sought to compare enzymatic activities under the influence of various temperature and pH regimes, as well as to carry out kinetic analyses of enzymatic reaction rates, maximum amounts of the reaction product, reaction times, maximum rates of the enzyme reactions, and Michaelis constants in cell-free extracts of intestinal SRB, D. piger Vib-7, and D. orale Rod-9, collected from exponential and stationary growth phases. The optimal temperature (35 °C) and pH (7.0) for both enzyme’s activity were determined. The difference in trends of Michaelis constants (Km) during exponential and stationary phases are noticeable between D. piger Vib-7 and D. orale Rod-9; D. orale Rod-9 showed much higher Km (the exception is NADH peroxidase of D. piger Vib-7: 1.42 ± 0.11 mM) during the both monitored phases. Studies of the NADH and NADPH peroxidases—as putative antioxidant defense systems of intestinal SRB and detailed data on the kinetic properties of this enzyme, as expressed by the decomposition of hydrogen peroxide—could be important for clarifying evolutionary mechanisms of antioxidant defense systems, their etiological role in the process of dissimilatory sulfate reduction, and their possible role in the development of bowel diseases.
ER  -

KUSHKEVYCH, Ivan; Dani DORDEVI$\backslash$'C; Mohammad I. ALBERFKANI; Márió GAJDÁCS; Eszter OSTORHÁZI; Monika VÍTĚZOVÁ a Simon K.-M. R. RITTMANN. NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria. \textit{Scientific Reports}. Nature Research, 2023, roč.~13, č.~1, s.~1-11. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-023-41185-3.

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