Light-responsive microRNA molecules in human retinal organoids
are differentially regulated by distinct wavelengths of light

J 2023

Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

ÇELIKER, Canan; Kamila WEISSOVÁ; Kateřina AMRUZ ČERNÁ; Jan OPPELT; Birthe DORGAU et. al.

Základní údaje

Originální název

Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

Autoři

ÇELIKER, Canan (792 Turecko, domácí); Kamila WEISSOVÁ (203 Česká republika, domácí); Kateřina AMRUZ ČERNÁ (203 Česká republika, domácí); Jan OPPELT (203 Česká republika); Birthe DORGAU; Francisco MOLINA GAMBIN (724 Španělsko, domácí); Jana ŠEBESTÍKOVÁ (203 Česká republika, domácí); Majlinda LAKO; Evelyne SERNAGOR; Petra LISKOVA (203 Česká republika) a Tomáš BÁRTA (203 Česká republika, garant, domácí)

Vydání

iSCIENCE, CAMBRIDGE, CELL PRESS, 2023, 2589-0042

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600

Kód RIV

RIV/00216224:14110/23:00131593

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.isci.2023.107237

UT WoS

001049218100001

EID Scopus

2-s2.0-85165984650

Klíčová slova anglicky

Light-responsive microRNA molecules; human retinal organoids

Štítky

14110517, CF CELLIM, CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2025 13:38, Mgr. Tereza Miškechová

Anotace

V originále

Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation to varying levels and wavelengths of light helps to regulate circadian rhythms and allows for adaptation to high levels of illumination, thereby enabling the rest of the visual system to remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role in regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs in human retinal cells remain unknown. Here, we aim to characterize these miRNAs. We generated light-responsive human retinal organoids that express miRNA families and clusters typically found in the retina. Using an in-house developed photostimulation device, we identified a subset of light regulated miRNAs. Importantly, we found that these miRNAs are differentially regulated by distinct wavelengths of light and have a rapid turnover, highlighting the dynamic and adaptive nature of the human retina.

Návaznosti

GA21-08182S, projekt VaV

Název: Retinální organoidy: Zkoumání účinků fotostimulace na lidskou sítnici

Investor: Grantová agentura ČR, Retinal Organoids: Exploring the Effects of Photo-Stimulation on Human Retina

LM2023067, projekt VaV

Název: Národní centrum lékařské genomiky

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, NCLG: Národní centrum lékařské genomiky

NU22-07-00380, projekt VaV

Název: Aplikace retinálních buněk a organoidů ve funkční diagnostice a léčbě ztráty zraku u Bardet-Biedlova syndromu

Investor: Ministerstvo zdravotnictví ČR, Aplikace retinálních buněk a organoidů ve funkční diagnostice a léčbě ztráty zraku u Bardet-Biedlova syndromu, Podprogram 1 - standardní

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

90267, velká výzkumná infrastruktura

Název: NCMG III

Citovat

ÇELIKER, Canan; Kamila WEISSOVÁ; Kateřina AMRUZ ČERNÁ; Jan OPPELT; Birthe DORGAU; Francisco MOLINA GAMBIN; Jana ŠEBESTÍKOVÁ; Majlinda LAKO; Evelyne SERNAGOR; Petra LISKOVA a Tomáš BÁRTA. Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light. iSCIENCE. CAMBRIDGE: CELL PRESS, 2023, roč. 26, č. 7, s. 1-20. ISSN 2589-0042. Dostupné z: https://dx.doi.org/10.1016/j.isci.2023.107237.

@article{2306618,
   author = {Çeliker, Canan and Weissová, Kamila and Amruz Černá, Kateřina and Oppelt, Jan and Dorgau, Birthe and Molina Gambin, Francisco and Šebestíková, Jana and Lako, Majlinda and Sernagor, Evelyne and Liskova, Petra and Bárta, Tomáš},
   article_location = {CAMBRIDGE},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.isci.2023.107237},
   keywords = {Light-responsive microRNA molecules; human retinal organoids},
   language = {eng},
   issn = {2589-0042},
   journal = {iSCIENCE},
   title = {Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light},
   url = {https://www.sciencedirect.com/science/article/pii/S2589004223013147?via%3Dihub},
   volume = {26},
   year = {2023}
}

TY  - JOUR
ID  - 2306618
AU  - Çeliker, Canan - Weissová, Kamila - Amruz Černá, Kateřina - Oppelt, Jan - Dorgau, Birthe - Molina Gambin, Francisco - Šebestíková, Jana - Lako, Majlinda - Sernagor, Evelyne - Liskova, Petra - Bárta, Tomáš
PY  - 2023
TI  - Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light
JF  - iSCIENCE
VL  - 26
IS  - 7
SP  - 1-20
EP  - 1-20
PB  - CELL PRESS
SN  - 25890042
KW  - Light-responsive microRNA molecules
KW  - human retinal organoids
UR  - https://www.sciencedirect.com/science/article/pii/S2589004223013147?via%3Dihub
N2  - Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation to varying levels and wavelengths of light helps to regulate circadian rhythms and allows for adaptation to high levels of illumination, thereby enabling the rest of the visual system to remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role in regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs in human retinal cells remain unknown. Here, we aim to characterize these miRNAs. We generated light-responsive human retinal organoids that express miRNA families and clusters typically found in the retina. Using an in-house developed photostimulation device, we identified a subset of light regulated miRNAs. Importantly, we found that these miRNAs are differentially regulated by distinct wavelengths of light and have a rapid turnover, highlighting the dynamic and adaptive nature of the human retina.
ER  -

$\backslash$C CELIKER, Canan; Kamila WEISSOVÁ; Kateřina AMRUZ ČERNÁ; Jan OPPELT; Birthe DORGAU; Francisco MOLINA GAMBIN; Jana ŠEBESTÍKOVÁ; Majlinda LAKO; Evelyne SERNAGOR; Petra LISKOVA a Tomáš BÁRTA. Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light. \textit{iSCIENCE}. CAMBRIDGE: CELL PRESS, 2023, roč.~26, č.~7, s.~1-20. ISSN~2589-0042. Dostupné z: https://dx.doi.org/10.1016/j.isci.2023.107237.

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