Palaeoproteomic and Genomic Analyses of Bronze Age Population
from Unětice Culture, Moravia, Czech Republic

a 2023

Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic

CHOCHOLOVÁ, Eva; Anna ŠENOVSKÁ; Pavel ROUDNICKÝ; Zbyněk ZDRÁHAL; Kristýna BRZOBOHATÁ et al.

Základní údaje

Originální název

Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic

Autoři

CHOCHOLOVÁ, Eva ; Anna ŠENOVSKÁ; Pavel ROUDNICKÝ; Zbyněk ZDRÁHAL; Kristýna BRZOBOHATÁ; Dana FIALOVÁ; Petr KOS a Eva DROZDOVÁ

Vydání

10th Meeting of the International Society for Biomolecular Archaeology, New Horizons in Biomolecular Archaeology, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00131733

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Metagenomika; 16S rRNA; paleoproteomika; starobylá DNA; aDNA; doba bronzová; mitogenom; Únětice

Klíčová slova anglicky

Metagenomics; 16S rRNA; palaeoproteomics; ancient DNA; aDNA; Bronze Age; Mitogenome; Únětice

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 9. 2023 15:47, Mgr. Eva Chocholová, Ph.D.

Anotace

V originále

Mitogenome research offers an intriguing insight into the history, origin, contacts, and variability of past populations and has become a standard part of the study of past populations. Ancient dental calculus is also a well-known and valuable source of information that is used to investigate health, diet, migrations, or habits in the context of biomolecular archaeology. The studied individuals were excavated at a Bronze Age site of Brno-Tuřany, Moravia region, Czech Republic. They belonged to the Unětice culture, dated to 2300-1600 BC. For the present study, we decided to combine both approaches: genomic analysis of mitochondrial DNA in 16 individuals and proteomic analysis of dental calculus in 8 individuals. Sampling, decontamination, ancient DNA extraction, library preparation, and the first stages of protein extraction were carried out in the specialised facility for ancient biomolecules in the Laboratory of Biological and Molecular Anthropology. DNA was purified using silica columns and the whole mitogenome libraries were prepared according to Šenovská et al. (2021) with mtDNA capture. The proteins were extracted with the FASP protocol modified for ancient biomolecules. LC-MS/MS analysis was performed on timsTOF Pro combined with nanoElute (Bruker). Based on mtDNA, the population from Brno-Tuřany was very variable and six general haplogroups were found: N (1), W (1), H (4), V (2), K (1), and the most frequent U (7). No shared haplotype occurred between the studied individuals. The identified dietary proteins originated from, e.g. Bos sp., Ovis/Capra sp., Triticum aestivum or Brassica sp. The biomolecular approach in archaeology therefore offers new insights into past populations.

Návaznosti

LM2015091, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

MUNI/A/1325/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Akronym: MBG10)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10

Citovat

CHOCHOLOVÁ, Eva; Anna ŠENOVSKÁ; Pavel ROUDNICKÝ; Zbyněk ZDRÁHAL; Kristýna BRZOBOHATÁ; Dana FIALOVÁ; Petr KOS a Eva DROZDOVÁ. Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic. In 10th Meeting of the International Society for Biomolecular Archaeology, New Horizons in Biomolecular Archaeology. 2023.

@proceedings{2313739,
   author = {Chocholová, Eva and Šenovská, Anna and Roudnický, Pavel and Zdráhal, Zbyněk and Brzobohatá, Kristýna and Fialová, Dana and Kos, Petr and Drozdová, Eva},
   booktitle = {10th Meeting of the International Society for Biomolecular Archaeology, New Horizons in Biomolecular Archaeology},
   keywords = {Metagenomics; 16S rRNA; palaeoproteomics; ancient DNA; aDNA; Bronze Age; Mitogenome; Únětice},
   language = {eng},
   title = {Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic},
   year = {2023}
}

TY  - CONF
ID  - 2313739
AU  - Chocholová, Eva - Šenovská, Anna - Roudnický, Pavel - Zdráhal, Zbyněk - Brzobohatá, Kristýna - Fialová, Dana - Kos, Petr - Drozdová, Eva
PY  - 2023
TI  - Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic
KW  - Metagenomics
KW  - 16S rRNA
KW  - palaeoproteomics
KW  - ancient DNA
KW  - aDNA
KW  - Bronze Age
KW  - Mitogenome
KW  - Únětice
N2  - Mitogenome research offers an intriguing insight into the history, origin, contacts, and variability of past populations and has become a standard part of the study of past populations. Ancient dental calculus is also a well-known and valuable source of information that is used to investigate health, diet, migrations, or habits in the context of biomolecular archaeology. The studied individuals were excavated at a Bronze Age site of Brno-Tuřany, Moravia region, Czech Republic. They belonged to the Unětice culture, dated to 2300-1600 BC. For the present study, we decided to combine both approaches: genomic analysis of mitochondrial DNA in 16 individuals and proteomic analysis of dental calculus in 8 individuals. Sampling, decontamination, ancient DNA extraction, library preparation, and the first stages of protein extraction were carried out in the specialised facility for ancient biomolecules in the Laboratory of Biological and Molecular Anthropology. DNA was purified using silica columns and the whole mitogenome libraries were prepared according to Šenovská et al. (2021) with mtDNA capture. The proteins were extracted with the FASP protocol modified for ancient biomolecules. LC-MS/MS analysis was performed on timsTOF Pro combined with nanoElute (Bruker). Based on mtDNA, the population from Brno-Tuřany was very variable and six general haplogroups were found: N (1), W (1), H (4), V (2), K (1), and the most frequent U (7). No shared haplotype occurred between the studied individuals. The identified dietary proteins originated from, e.g. Bos sp., Ovis/Capra sp., Triticum aestivum or Brassica sp. The biomolecular approach in archaeology therefore offers new insights into past populations.
ER  -

CHOCHOLOVÁ, Eva; Anna ŠENOVSKÁ; Pavel ROUDNICKÝ; Zbyněk ZDRÁHAL; Kristýna BRZOBOHATÁ; Dana FIALOVÁ; Petr KOS a Eva DROZDOVÁ. Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic. In \textit{10th Meeting of the International Society for Biomolecular Archaeology, New Horizons in Biomolecular Archaeology}. 2023.

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