2023
Neutrophil and eosinophil granulocyte immunophenotyping and a burst test
ŠTÍCHOVÁ, Julie; Peter SLANINA; Zita CHOVANCOVÁ; Karolína SURÁ; Jana NECHVÁTALOVÁ et al.Základní údaje
Originální název
Neutrophil and eosinophil granulocyte immunophenotyping and a burst test
Název česky
Imunofenotypizace a burst test u neutrofilních granulocytů a eozinofilů
Autoři
ŠTÍCHOVÁ, Julie; Peter SLANINA; Zita CHOVANCOVÁ; Karolína SURÁ a Jana NECHVÁTALOVÁ
Vydání
12th International Conference Analytical Cytometry, 2023
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30102 Immunology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14110/23:00134650
Organizační jednotka
Lékařská fakulta
Klíčová slova česky
Neutrofilní granulocyt; eozinofilní granulocyt; burst test; imunofenotypizace; astma; průtoková cytometrie; operace kyčelního kloubu
Klíčová slova anglicky
Neutrophil granulocyte; eosinophil granulocyte; burst test; immunophenotyping; flow cytometry; allergic asthma; hip surgery
Změněno: 6. 5. 2024 09:14, Mgr. Tereza Miškechová
Anotace
V originále
Neutrophils and eosinophils are among the largest granulocytes observed in clinical flow cytometry. They are commonly gated from the basic FSC vs SSC dot plot based on their size and granularity, or by using the pan-leukocyte antigen CD45 and SSC. Routine laboratories usually measure only the relative numbers of neutrophils or eosinophils. In terms of functional tests, these include the test of oxidative burst in neutrophils to rule out the diagnosis of chronic granulomatosis, or the test for the presence of myeloperoxidase (MPO) in case of suspected immunodeficiency of MPO. Inflammation or bone marrow disorders are the most common causes of increased neutrophil representation and an increased presence of immature neutrophils in peripheral blood (PB). Eosinophil granulocytes are commonly increased in PB of various diseases including allergic eosinophilic asthma, allergic eczema, parasite infections or immunodeficiencies like Omenn syndrome and hyper IgE syndrome. Our study aimed to perform a functional test of the oxidative burst of neutrophils and eosinophils with their simultaneous phenotyping in patients with eosinophilic asthma and patients after hip surgery. The results showed that immature neutrophils and eosinophils have a reduced oxidative burst capacity compared to their mature forms. Immunophenotyping of neutrophils and eosinophils allows the recognition of their mature and immature forms, which differ in their functional properties. Our findings could help in the interpretation of findings in patients with partially reduced oxidative burst.
Návaznosti
| MUNI/A/1098/2022, interní kód MU |
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| NU20-05-00146, projekt VaV |
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| NU21-06-00408, projekt VaV |
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