Molecular profiling of the vestibular lamina highlights a key
role for Hedgehog signalling

J 2023

Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling

QIU, Tengyang; Barbora HUTEČKOVÁ; Maisa SEPPALA; Martyn T COBOURNE; Zhi CHEN et al.

Základní údaje

Originální název

Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling

Autoři

QIU, Tengyang; Barbora HUTEČKOVÁ; Maisa SEPPALA; Martyn T COBOURNE; Zhi CHEN; Mária HOVOŘÁKOVÁ; Marcela BUCHTOVÁ a Abigail S TUCKER

Vydání

Development, Company of Biologists Ltd, 2023, 0950-1991

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00134235

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Vestibular lamina; Oral cavity; Sonic hedgehog; Gas1; Dental lamina; Ciliopathies; Mouse

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 9. 2023 10:10, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The vestibular lamina (VL) forms the oral vestibule, creating a gap between the teeth, lips and cheeks. In a number of ciliopathies, formation of the vestibule is defective, leading to the creation of multiple frenula. In contrast to the neighbouring dental lamina, which forms the teeth, little is known about the genes that pattern the VL. Here, we establish a molecular signature for the usually non-odontogenic VL in mice and highlight several genes and signalling pathways that may play a role in its development. For one of these, the Sonic hedgehog (Shh) pathway, we show that co-receptors Gas1, Cdon and Boc are highly expressed in the VL and act to enhance the Shh signal from the forming incisor region. In Gas1 mutant mice, expression of Gli1 was disrupted and the VL epithelium failed to extend due to a loss of proliferation. This defect was exacerbated in Boc/Gas1 double mutants and could be phenocopied using cyclopamine in culture. Signals from the forming teeth, therefore, control development of the VL, coordinating the development of the dentition and the oral cavity.

Návaznosti

GA21-04178S, projekt VaV

Název: Propojení funkce Sprouty s FGF a primárními ciliemi ve vývoji

Investor: Grantová agentura ČR, Propojení funkce Sprouty s FGF a primárními ciliemi ve vývoji

Citovat

QIU, Tengyang; Barbora HUTEČKOVÁ; Maisa SEPPALA; Martyn T COBOURNE; Zhi CHEN; Mária HOVOŘÁKOVÁ; Marcela BUCHTOVÁ a Abigail S TUCKER. Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling. Development. Company of Biologists Ltd, 2023, roč. 150, č. 7, s. 1-13. ISSN 0950-1991. Dostupné z: https://doi.org/10.1242/dev.201464.

@article{2314698,
   author = {Qiu, Tengyang and Hutečková, Barbora and Seppala, Maisa and Cobourne, Martyn T and Chen, Zhi and Hovořáková, Mária and Buchtová, Marcela and Tucker, Abigail S},
   article_number = {7},
   doi = {https://doi.org/10.1242/dev.201464},
   keywords = {Vestibular lamina; Oral cavity; Sonic hedgehog; Gas1; Dental lamina; Ciliopathies; Mouse},
   language = {eng},
   issn = {0950-1991},
   journal = {Development},
   title = {Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling},
   url = {https://doi.org/10.1242/dev.201464},
   volume = {150},
   year = {2023}
}

TY  - JOUR
ID  - 2314698
AU  - Qiu, Tengyang - Hutečková, Barbora - Seppala, Maisa - Cobourne, Martyn T - Chen, Zhi - Hovořáková, Mária - Buchtová, Marcela - Tucker, Abigail S
PY  - 2023
TI  - Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling
JF  - Development
VL  - 150
IS  - 7
SP  - 1-13
EP  - 1-13
PB  - Company of Biologists Ltd
SN  - 09501991
KW  - Vestibular lamina
KW  - Oral cavity
KW  - Sonic hedgehog
KW  - Gas1
KW  - Dental lamina
KW  - Ciliopathies
KW  - Mouse
UR  - https://doi.org/10.1242/dev.201464
N2  - The vestibular lamina (VL) forms the oral vestibule, creating a gap between the teeth, lips and cheeks. In a number of ciliopathies, formation of the vestibule is defective, leading to the creation of multiple frenula. In contrast to the neighbouring dental lamina, which forms the teeth, little is known about the genes that pattern the VL. Here, we establish a molecular signature for the usually non-odontogenic VL in mice and highlight several genes and signalling pathways that may play a role in its development. For one of these, the Sonic hedgehog (Shh) pathway, we show that co-receptors Gas1, Cdon and Boc are highly expressed in the VL and act to enhance the Shh signal from the forming incisor region. In Gas1 mutant mice, expression of Gli1 was disrupted and the VL epithelium failed to extend due to a loss of proliferation. This defect was exacerbated in Boc/Gas1 double mutants and could be phenocopied using cyclopamine in culture. Signals from the forming teeth, therefore, control development of the VL, coordinating the development of the dentition and the oral cavity.
ER  -

QIU, Tengyang; Barbora HUTEČKOVÁ; Maisa SEPPALA; Martyn T COBOURNE; Zhi CHEN; Mária HOVOŘÁKOVÁ; Marcela BUCHTOVÁ a Abigail S TUCKER. Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling. \textit{Development}. Company of Biologists Ltd, 2023, roč.~150, č.~7, s.~1-13. ISSN~0950-1991. Dostupné z: https://doi.org/10.1242/dev.201464.

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