Segmented Hair Cortisol Analysis By Online Solid Phase
Extraction Liquid Chromatography Mass Spectrometry

a 2023

Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry

ŠMAK, Pavel, Daniela FAČKOVCOVÁ, Katarína KOSTOLANSKÁ, Jana GREGOROVÁ, Eliška BARTEČKOVÁ et. al.

Základní údaje

Originální název

Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry

Autoři

ŠMAK, Pavel (203 Česká republika, domácí), Daniela FAČKOVCOVÁ (703 Slovensko, domácí), Katarína KOSTOLANSKÁ (703 Slovensko, domácí), Jana GREGOROVÁ (203 Česká republika, domácí), Eliška BARTEČKOVÁ (203 Česká republika, domácí), Jan JUŘICA (203 Česká republika, domácí), Libor USTOHAL (203 Česká republika, domácí), Jana HOŘÍNKOVÁ (203 Česká republika, domácí), Pavla LINHARTOVÁ (203 Česká republika) a Ondřej PEŠ (203 Česká republika, domácí)

Vydání

24. Škola hmotnostní spektrometrie 2023, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/23:00131876

Organizační jednotka

Lékařská fakulta

ISBN

978-80-88195-44-3

Klíčová slova anglicky

hair cortisol; liquid chromatography; mass spectrometry; probabilistic; Bayesian; longitudinal; evaluation

Změněno: 10. 4. 2024 13:02, Mgr. Tereza Miškechová

Anotace

V originále

The hypothalamic-pituitary-adrenal axis is the primary neurocrine axis that regulates response of the stress. Activation of this axis leads to releasing glucocorticoid (cortisol) that regulates physiological events and inhibits further HPA axis activation. Dysregulation of the hypothalamic-pituitary-adrenal axis plays an important role in a number of endocrine and psychiatric disorders, such as adrenal insufficiency, (ectopic) Cushing syndrome, major depressive disorder, borderline personality disorder, or schizophrenia. Hair cortisol concentration (HCC) belongs to relatively new methods of estimating cumulative cortisol exposure over months. While plasma, saliva, and urine cortisol levels provide immediate values in the organism, hair matrix allows for chronic exposure evaluation and longitudinal monitoring. Large inter- and intraindividual variability of the hair sampling and determination could be overcome by a segmentation approach – cutting the hair strand into smaller segments and analyzing them independently. Online (on-column) solid phase extraction liquid chromatography mass spectrometry (SPE LC MS) using a surrogate analyte has been performed to quantify HCC in various segmented hair samples. Based on the hair length, it was possible to obtain a relatively high number (3–6) of segments possibly containing highly correlated HCC values. Probabilistic multilevel (hierarchical) models were applied to volunteers’ hair for establishing a parameter of decrease, which could then be used to correct for such a decrease in the hair of the studied population. Consequently, the deviations from the corrections could then be attributed to the effect of fluctuation or variability of HCC in a certain time point (segment), e.g. episode, intervention, etc.

Návaznosti

MUNI/A/1275/2022, interní kód MU

Název: Příspěvek chemických a biochemických technik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění

Investor: Masarykova univerzita, Příspěvek chemických a biochemických technik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění

NU23-08-00257, projekt VaV

Název: Optimalizovaný longitudinální vlasový kortizol jako nástroj v diagnostice a monitorování psychiatrických poruch

Investor: Ministerstvo zdravotnictví ČR, Optimalizovaný longitudinální vlasový kortizol jako nástroj v diagnostice a monitorování psychiatrických poruch, Podprogram 1 - standardní

Citovat

ŠMAK, Pavel, Daniela FAČKOVCOVÁ, Katarína KOSTOLANSKÁ, Jana GREGOROVÁ, Eliška BARTEČKOVÁ, Jan JUŘICA, Libor USTOHAL, Jana HOŘÍNKOVÁ, Pavla LINHARTOVÁ a Ondřej PEŠ. Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry. In 24. Škola hmotnostní spektrometrie 2023. 2023. ISBN 978-80-88195-44-3.

@proceedings{2324060,
   author = {Šmak, Pavel and Fačkovcová, Daniela and Kostolanská, Katarína and Gregorová, Jana and Bartečková, Eliška and Juřica, Jan and Ustohal, Libor and Hořínková, Jana and Linhartová, Pavla and Peš, Ondřej},
   booktitle = {24. Škola hmotnostní spektrometrie 2023},
   keywords = {hair cortisol; liquid chromatography; mass spectrometry; probabilistic; Bayesian; longitudinal; evaluation},
   language = {eng},
   isbn = {978-80-88195-44-3},
   title = {Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry},
   url = {http://skolams2023.spektroskopie.cz/},
   year = {2023}
}

TY  - CONF
ID  - 2324060
AU  - Šmak, Pavel - Fačkovcová, Daniela - Kostolanská, Katarína - Gregorová, Jana - Bartečková, Eliška - Juřica, Jan - Ustohal, Libor - Hořínková, Jana - Linhartová, Pavla - Peš, Ondřej
PY  - 2023
TI  - Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry
SN  - 9788088195443
KW  - hair cortisol
KW  - liquid chromatography
KW  - mass spectrometry
KW  - probabilistic
KW  - Bayesian
KW  - longitudinal
KW  - evaluation
UR  - http://skolams2023.spektroskopie.cz/
N2  - The hypothalamic-pituitary-adrenal axis is the primary neurocrine axis that regulates response of the stress. Activation of this axis leads to releasing glucocorticoid (cortisol) that regulates physiological events and inhibits further HPA axis activation. Dysregulation of the hypothalamic-pituitary-adrenal axis plays an important role in a number of endocrine and psychiatric disorders, such as adrenal insufficiency, (ectopic) Cushing syndrome, major depressive disorder, borderline personality disorder, or schizophrenia. Hair cortisol concentration (HCC) belongs to relatively new methods of estimating cumulative cortisol exposure over months. While plasma, saliva, and urine cortisol levels provide immediate values in the organism, hair matrix allows for chronic exposure evaluation and longitudinal monitoring. Large inter- and intraindividual variability of the hair sampling and determination could be overcome by a segmentation approach – cutting the hair strand into smaller segments and analyzing them independently. Online (on-column) solid phase extraction liquid chromatography mass spectrometry (SPE LC MS) using a surrogate analyte has been performed to quantify HCC in various segmented hair samples. Based on the hair length, it was possible to obtain a relatively high number (3–6) of segments possibly containing highly correlated HCC values. Probabilistic multilevel (hierarchical) models were applied to volunteers’ hair for establishing a parameter of decrease, which could then be used to correct for such a decrease in the hair of the studied population. Consequently, the deviations from the corrections could then be attributed to the effect of fluctuation or variability of HCC in a certain time point (segment), e.g. episode, intervention, etc.
ER  -

ŠMAK, Pavel, Daniela FAČKOVCOVÁ, Katarína KOSTOLANSKÁ, Jana GREGOROVÁ, Eliška BARTEČKOVÁ, Jan JUŘICA, Libor USTOHAL, Jana HOŘÍNKOVÁ, Pavla LINHARTOVÁ a Ondřej PEŠ. Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry. In \textit{24. Škola hmotnostní spektrometrie 2023}. 2023. ISBN~978-80-88195-44-3.

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