Další formáty:
BibTeX
LaTeX
RIS
@article{2335177,
author = {Lesiczka, Paulina Maria and Hrazdilova, Kristyna and Hönig, Vaclav and Modrý, David and Zurek, Ludek},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s13071-023-05654-y},
keywords = {Anaplasma phagocytophilum; Ixodes ricinus; Anaplasmosis; Genetic diversity; Infectious diseases},
language = {eng},
issn = {1756-3305},
journal = {Parasites and Vectors},
title = {Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people},
url = {https://doi.org/10.1186/s13071-023-05654-y},
volume = {16},
year = {2023}
}
TY - JOUR
ID - 2335177
AU - Lesiczka, Paulina Maria - Hrazdilova, Kristyna - Hönig, Vaclav - Modrý, David - Zurek, Ludek
PY - 2023
TI - Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people
JF - Parasites and Vectors
VL - 16
IS - 1
SP - 1-10
EP - 1-10
PB - BioMed Central Ltd
SN - 17563305
KW - Anaplasma phagocytophilum
KW - Ixodes ricinus
KW - Anaplasmosis
KW - Genetic diversity
KW - Infectious diseases
UR - https://doi.org/10.1186/s13071-023-05654-y
N2 - Background Although the tick-borne pathogen Anaplasma phagocytophilum is currently described as a single species, studies using genetic markers can distinguish groups of variants associated with different hosts, pathogenicity, zoonotic potential and biotic and geographic niches. The objective of our study was to investigate the genetic diversity of A. phagocytophilum and Ixodes ricinus ticks attached to people.Methods In collaboration with a commercial diagnostic company, a total of 52 DNA samples were obtained from ticks that tested positive for A. phagocytophilum by quantitative PCR. The genetic profile of each sample was determined using the groEL and ankA genes. Identification of the tick species was confirmed by partial sequencing of the COI subunit and a portion of the TROSPA gene.Results All 52 ticks were identified as I. ricinus. Two protocols of nested PCR amplifying 1293- and 407-bp fragments of groEL of A. phagocytophilum yielded amplicons of the expected size for all 52 samples. Among all sequences, we identified 10 unique genetic variants of groEL belonging to ecotype I and ecotype II. The analysis targeting ankA was successful in 46 of 52 ticks. Among all sequences, we identified 21 unique genetic variants phylogenetically belonging to three clusters.Conclusions Our results indicate that ticks attached to people harbor distant genetic variants of A. phagocytophilum, some of which are not recognized as zoonotic. Further studies are needed to determine the risk of human infection by genetic variants other than those designated as zoonotic.
ER -
LESICZKA, Paulina Maria, Kristyna HRAZDILOVA, Vaclav HÖNIG, David MODRÝ a Ludek ZUREK. Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people. \textit{Parasites and Vectors}. BioMed Central Ltd, 2023, roč.~16, č.~1, s.~1-10. ISSN~1756-3305. Dostupné z: https://dx.doi.org/10.1186/s13071-023-05654-y.
|
