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@article{2343978,
author = {Vaculík, Ondřej and Bernatová, Silvie and Rebrošová, Katarína and Samek, Ota and Šilhan, Lukáš and Růžička, Filip and Šerý, Mojmír and Šiler, Martin and Ježek, Jan and Zemánek, Pavel},
article_location = {WASHINGTON},
article_number = {12},
doi = {http://dx.doi.org/10.1364/BOE.503628},
keywords = {Raman tweezers; blood serum; pathogens},
language = {eng},
issn = {2156-7085},
journal = {Biomedical Optics Express},
title = {Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods},
url = {https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-12-6410&id=542842#},
volume = {14},
year = {2023}
}
TY - JOUR
ID - 2343978
AU - Vaculík, Ondřej - Bernatová, Silvie - Rebrošová, Katarína - Samek, Ota - Šilhan, Lukáš - Růžička, Filip - Šerý, Mojmír - Šiler, Martin - Ježek, Jan - Zemánek, Pavel
PY - 2023
TI - Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods
JF - Biomedical Optics Express
VL - 14
IS - 12
SP - 6410-6421
EP - 6410-6421
PB - OPTICA PUBLISHING GROUP
SN - 21567085
KW - Raman tweezers
KW - blood serum
KW - pathogens
UR - https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-12-6410&id=542842#
N2 - Pathogenic microbes contribute to several major global diseases that kill millions of people every year. Bloodstream infections caused by these microbes are associated with high morbidity and mortality rates, which are among the most common causes of hospitalizations. The search for the “Holy Grail” in clinical diagnostic microbiology, a reliable, accurate, low cost, real-time, and easy-to-use diagnostic method, is one of the essential issues in clinical practice. These very critical conditions can be met by Raman tweezers in combination with advanced analysis methods. Here, we present a proof-of-concept study based on Raman tweezers combined with spectral mixture analysis that allows for the identification of microbial strains directly from human blood serum without user intervention, thus eliminating the influence of a data analyst.
ER -
VACULÍK, Ondřej, Silvie BERNATOVÁ, Katarína REBROŠOVÁ, Ota SAMEK, Lukáš ŠILHAN, Filip RŮŽIČKA, Mojmír ŠERÝ, Martin ŠILER, Jan JEŽEK a Pavel ZEMÁNEK. Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods. \textit{Biomedical Optics Express}. WASHINGTON: OPTICA PUBLISHING GROUP, 2023, roč.~14, č.~12, s.~6410-6421. ISSN~2156-7085. Dostupné z: https://dx.doi.org/10.1364/BOE.503628.
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