Uncovering biosynthetic potential of soil bacteria in
Antarctica

k 2022

Uncovering biosynthetic potential of soil bacteria in Antarctica

KRÁLOVÁ, Stanislava; Songcan CHEN; Mathias FLIEDER; Matěj BEZDÍČEK; Jana HANSLIKOVÁ et al.

Základní údaje

Originální název

Uncovering biosynthetic potential of soil bacteria in Antarctica

Autoři

KRÁLOVÁ, Stanislava; Songcan CHEN; Mathias FLIEDER; Matěj BEZDÍČEK; Jana HANSLIKOVÁ; Thomas RATTEI a Alexander LOY

Vydání

18th International Symposium on Microbial Ecology (ISME18), 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00132598

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Antarktida; biosynteticky potencial; pudní bakterie, antimikrobiální látky; resistance

Klíčová slova anglicky

Antarctica; biosynthetic potential; soil bacteria; antimicrobials; resistance

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 12. 2023 09:40, RNDr. Stanislava Bezdíček Králová, Ph.D.

Anotace

V originále

Antarctica represents an extreme, pristine, and hardly accessible environment. Bacteria inhabiting Antarctic ecosystems developed a plethora of adaptation mechanisms, including the ability to produce secondary metabolites to fight competitors or sustain abiotic stresses. These metabolites represent a remarkable source of natural products for pharmaceutical use. Therefore, bioprospecting of endemic Antarctic bacteria may uncover novel natural products with potential to combat multidrug-resistant pathogens whose incidence is rapidly rising. Here, three isolation methods have been applied to recover pure cultures of novel bacteria from Antarctic soils; with focus on phyla known for their high biosynthetic potential. Isolation procedures based on activation of sporulation to select for spore-forming bacteria (Bacillota, Actinobacteriota), media supporting actinobacterial growth, or low-nutrient and soil-extract based media to obtain fastidious strains. Phylogenetic/genomic methods are applied to analyse the taxonomic novelty of isolates and their potential to produce novel secondary metabolites. A collection of 522, mostly slow growing isolates was established. Most of these strains (60%) belonged to the phyla Actinobacteriota and Bacillota, and predominantly represented novel species of the genera Streptomyces, Pillimelia, Pseudoarthrobacter, Arthrobacter, Nocardioides, Bacillus, and Paenibacillus. Along with these, Pseudomonadota represented the next most abundant phylum in the strain collection, including several new species and one new genus. Application of specific isolation methods and longer cultivation times recovered several heretofore unknown bacterial taxa, most of which are related to taxa well-known as producers of antimicrobial compounds and promising for further exploitation.

Návaznosti

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

Citovat

KRÁLOVÁ, Stanislava; Songcan CHEN; Mathias FLIEDER; Matěj BEZDÍČEK; Jana HANSLIKOVÁ; Thomas RATTEI a Alexander LOY. Uncovering biosynthetic potential of soil bacteria in Antarctica. In 18th International Symposium on Microbial Ecology (ISME18). 2022.

@proceedings{2351919,
   author = {Králová, Stanislava and Chen, Songcan and Flieder, Mathias and Bezdíček, Matěj and Hansliková, Jana and Rattei, Thomas and Loy, Alexander},
   booktitle = {18th International Symposium on Microbial Ecology (ISME18)},
   keywords = {Antarctica; biosynthetic potential; soil bacteria; antimicrobials; resistance},
   language = {eng},
   title = {Uncovering biosynthetic potential of soil bacteria in Antarctica},
   year = {2022}
}

TY  - CONF
ID  - 2351919
AU  - Králová, Stanislava - Chen, Songcan - Flieder, Mathias - Bezdíček, Matěj - Hansliková, Jana - Rattei, Thomas - Loy, Alexander
PY  - 2022
TI  - Uncovering biosynthetic potential of soil bacteria in Antarctica
KW  - Antarctica
KW  - biosynthetic potential
KW  - soil bacteria
KW  - antimicrobials
KW  - resistance
N2  - Antarctica represents an extreme, pristine, and hardly accessible environment. Bacteria inhabiting Antarctic ecosystems developed a plethora of adaptation mechanisms, including the ability to produce secondary metabolites to fight competitors or sustain abiotic stresses. These metabolites represent a remarkable source of natural products for pharmaceutical use. Therefore, bioprospecting of endemic Antarctic bacteria may uncover novel natural products with potential to combat multidrug-resistant pathogens whose incidence is rapidly rising. Here, three isolation methods have been applied to recover pure cultures of novel bacteria from Antarctic soils; with focus on phyla known for their high biosynthetic potential. Isolation procedures based on activation of sporulation to select for spore-forming bacteria (Bacillota, Actinobacteriota), media supporting actinobacterial growth, or low-nutrient and soil-extract based media to obtain fastidious strains. Phylogenetic/genomic methods are applied to analyse the taxonomic novelty of isolates and their potential to produce novel secondary metabolites. A collection of 522, mostly slow growing isolates was established. Most of these strains (60%) belonged to the phyla Actinobacteriota and Bacillota, and predominantly represented novel species of the genera Streptomyces, Pillimelia, Pseudoarthrobacter, Arthrobacter, Nocardioides, Bacillus, and Paenibacillus. Along with these, Pseudomonadota represented the next most abundant phylum in the strain collection, including several new species and one new genus. Application of specific isolation methods and longer cultivation times recovered several heretofore unknown bacterial taxa, most of which are related to taxa well-known as producers of antimicrobial compounds and promising for further exploitation.
ER  -

KRÁLOVÁ, Stanislava; Songcan CHEN; Mathias FLIEDER; Matěj BEZDÍČEK; Jana HANSLIKOVÁ; Thomas RATTEI a Alexander LOY. Uncovering biosynthetic potential of soil bacteria in Antarctica. In \textit{18th International Symposium on Microbial Ecology (ISME18)}. 2022.

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