Exploring the Icy Treasures: Biosynthetic potential of
Antarctic bacteria from James Ross Island

D 2023

Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island

BEZDÍČEK KRÁLOVÁ, Stanislava; Mathias FLIEDER; Songcan CHEN; Peter ŠPAČEK; Natalia STANIC et al.

Základní údaje

Originální název

Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island

Autoři

BEZDÍČEK KRÁLOVÁ, Stanislava; Mathias FLIEDER; Songcan CHEN; Peter ŠPAČEK; Natalia STANIC; Matěj BEZDÍČEK; Martin ZEHL; Sergey ZOTCHEV; Thomas RATTEI a Alexander LOY

Vydání

Brno, Proceedings: Students in Polar and Alpine Research Conference 2023, od s. 4-4, 1 s. 2023

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0456-9

Klíčová slova česky

Antarktida; biosynteticky potencial; nové antimikrobiální látky; Streptomyces; Actinokineospora; genetické manipulace

Klíčová slova anglicky

Antarctica; biosynthetic potential; novel antimicrobials; Streptomyces; Actinokineospora; genetic manipulations

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 4. 2024 14:13, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background: The growing problem of antimicrobial resistance motivated scientists to re-explore natural sources of bioactive secondary metabolites. Antarctica represents an extreme environment colonized by bacteria with unique adaptation mechanisms allowing them to thrive under harsh conditions. Such adaptations include production of secondary metabolites to inhibit competitors or sustain abiotic stresses, which predestines these microbes as source of natural products for biomedical use. Objectives: The aim of this work was to recover novel bacterial taxa from Antarctic soils to access the biosynthetic potential hidden in yet uncultivated bacteria. The main objective is the activation of silent biosynthetic gene clusters enabling discovery of novel secondary metabolites, mainly through co-cultivation strategies. Methods: Three isolation methods (pre-selection of spore-forming bacteria, low-nutrient and soil-extract based media) were applied to recover novel bacteria from Antarctic soils, predominantly targeting phyla with high biosynthetic potential such as Actinobacteriota, Proteobacteria and Firmicutes. Activation of silent biosynthetic gene clusters was attempted through targeted cultivation and co-cultivation. Screening for bioactive molecules and evaluation of their novelty was achieved by application of genomics, metabolomics, and bioactivity testing. Results: A collection of 917 isolates was established. Recovered isolates were associated with four bacterial phyla including 77 isolates of novel species. Proteobacteria and Actinobacteriota represented the most abundant phyla. Specific media stimulated biosynthesis of several unknown natural products. Eight strains produced antimicrobial compounds against resistant and multidrug-resistant bacterial and fungal pathogens. Importantly, metabolomic profiling indicated that these strains produced several new secondary metabolites, which may be responsible for the observed antimicrobial activities.

Návaznosti

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

Citovat

BEZDÍČEK KRÁLOVÁ, Stanislava; Mathias FLIEDER; Songcan CHEN; Peter ŠPAČEK; Natalia STANIC; Matěj BEZDÍČEK; Martin ZEHL; Sergey ZOTCHEV; Thomas RATTEI a Alexander LOY. Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island. Online. In Jakub Holuša, Martin Kadlec, Christopher D. Stringer. Proceedings: Students in Polar and Alpine Research Conference 2023. Brno: Masaryk University, 2023, s. 4-4. ISBN 978-80-280-0456-9.

@inproceedings{2351944,
   author = {Bezdíček Králová, Stanislava and Flieder, Mathias and Chen, Songcan and Špaček, Peter and Stanic, Natalia and Bezdíček, Matěj and Zehl, Martin and Zotchev, Sergey and Rattei, Thomas and Loy, Alexander},
   address = {Brno},
   booktitle = {Proceedings: Students in Polar and Alpine Research Conference 2023},
   editor = {Jakub Holuša, Martin Kadlec, Christopher D. Stringer},
   keywords = {Antarctica; biosynthetic potential; novel antimicrobials; Streptomyces; Actinokineospora; genetic manipulations},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-280-0456-9},
   pages = {4-4},
   publisher = {Masaryk University},
   title = {Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island},
   url = {https://munispace.muni.cz/library/catalog/view/2259/6879/4475-1/0#preview},
   year = {2023}
}

TY  - CONF
ID  - 2351944
AU  - Bezdíček Králová, Stanislava - Flieder, Mathias - Chen, Songcan - Špaček, Peter - Stanic, Natalia - Bezdíček, Matěj - Zehl, Martin - Zotchev, Sergey - Rattei, Thomas - Loy, Alexander
PY  - 2023
TI  - Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island
PB  - Masaryk University
CY  - Brno
SN  - 9788028004569
KW  - Antarctica
KW  - biosynthetic potential
KW  - novel antimicrobials
KW  - Streptomyces
KW  - Actinokineospora
KW  - genetic manipulations
UR  - https://munispace.muni.cz/library/catalog/view/2259/6879/4475-1/0#preview
N2  - Background: The growing problem of antimicrobial resistance motivated scientists to re-explore natural sources of bioactive secondary metabolites. Antarctica represents an extreme environment colonized by bacteria with unique adaptation mechanisms allowing them to thrive under harsh conditions. Such adaptations include production of secondary metabolites to inhibit competitors or sustain abiotic stresses, which predestines these microbes as source of natural products for biomedical use. Objectives: The aim of this work was to recover novel bacterial taxa from Antarctic soils to access the biosynthetic potential hidden in yet uncultivated bacteria. The main objective is the activation of silent biosynthetic gene clusters enabling discovery of novel secondary metabolites, mainly through co-cultivation strategies. Methods: Three isolation methods (pre-selection of spore-forming bacteria, low-nutrient and soil-extract based media) were applied to recover novel bacteria from Antarctic soils, predominantly targeting phyla with high biosynthetic potential such as Actinobacteriota, Proteobacteria and Firmicutes. Activation of silent biosynthetic gene clusters was attempted through targeted cultivation and co-cultivation. Screening for bioactive molecules and evaluation of their novelty was achieved by application of genomics, metabolomics, and bioactivity testing. Results: A collection of 917 isolates was established. Recovered isolates were associated with four bacterial phyla including 77 isolates of novel species. Proteobacteria and Actinobacteriota represented the most abundant phyla. Specific media stimulated biosynthesis of several unknown natural products. Eight strains produced antimicrobial compounds against resistant and multidrug-resistant bacterial and fungal pathogens. Importantly, metabolomic profiling indicated that these strains produced several new secondary metabolites, which may be responsible for the observed antimicrobial activities.
ER  -

BEZDÍČEK KRÁLOVÁ, Stanislava; Mathias FLIEDER; Songcan CHEN; Peter ŠPAČEK; Natalia STANIC; Matěj BEZDÍČEK; Martin ZEHL; Sergey ZOTCHEV; Thomas RATTEI a Alexander LOY. Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island. Online. In Jakub Holuša, Martin Kadlec, Christopher D. Stringer. \textit{Proceedings: Students in Polar and Alpine Research Conference 2023}. Brno: Masaryk University, 2023, s.~4-4. ISBN~978-80-280-0456-9.

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