A scoring system for histological evaluation of skin during
Staphylococcus aureus infections

J 2024

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ et. al.

Základní údaje

Originální název

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

Autoři

MAKOVICKÝ, Peter (703 Slovensko), Edita JEKLOVÁ (203 Česká republika), Břetislav LIPOVÝ (203 Česká republika, domácí), Filip RAŠKA (203 Česká republika, domácí), Martin KNOZ (203 Česká republika, domácí), Rea JAROSOVA (203 Česká republika), Šárka KOBZOVÁ (203 Česká republika), Lukáš VACEK (203 Česká republika, domácí), Adam NOREK (203 Česká republika) a Lubomír JANDA (203 Česká republika)

Vydání

Biologia, New York, Springer, 2024, 0006-3088

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30303 Infectious Diseases

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.500 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s11756-023-01567-4

UT WoS

001105224900001

Klíčová slova anglicky

Bacterial infection; Histology; MRSA; Non-healing wounds; S. aureus

Štítky

14110113, 14110128, 14110229, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 7. 2024 12:44, Mgr. Tereza Miškechová

Anotace

V originále

The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.

Návaznosti

NU20-05-00166, projekt VaV

Název: Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií (Akronym: NPHIRPHGK)

Investor: Ministerstvo zdravotnictví ČR, Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií, Podprogram 1 - standardní

NU22-05-00475, projekt VaV

Název: Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech

Investor: Ministerstvo zdravotnictví ČR, Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech, Podprogram 1 - standardní

Citovat

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ, Rea JAROSOVA, Šárka KOBZOVÁ, Lukáš VACEK, Adam NOREK a Lubomír JANDA. A scoring system for histological evaluation of skin during Staphylococcus aureus infections. Biologia. New York: Springer, 2024, roč. 79, č. 3, s. 917-925. ISSN 0006-3088. Dostupné z: https://dx.doi.org/10.1007/s11756-023-01567-4.

@article{2352217,
   author = {Makovický, Peter and Jeklová, Edita and Lipový, Břetislav and Raška, Filip and Knoz, Martin and Jarosova, Rea and Kobzová, Šárka and Vacek, Lukáš and Norek, Adam and Janda, Lubomír},
   article_location = {New York},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11756-023-01567-4},
   keywords = {Bacterial infection; Histology; MRSA; Non-healing wounds; S. aureus},
   language = {eng},
   issn = {0006-3088},
   journal = {Biologia},
   title = {A scoring system for histological evaluation of skin during Staphylococcus aureus infections},
   url = {https://link.springer.com/article/10.1007/s11756-023-01567-4},
   volume = {79},
   year = {2024}
}

TY  - JOUR
ID  - 2352217
AU  - Makovický, Peter - Jeklová, Edita - Lipový, Břetislav - Raška, Filip - Knoz, Martin - Jarosova, Rea - Kobzová, Šárka - Vacek, Lukáš - Norek, Adam - Janda, Lubomír
PY  - 2024
TI  - A scoring system for histological evaluation of skin during Staphylococcus aureus infections
JF  - Biologia
VL  - 79
IS  - 3
SP  - 917-925
EP  - 917-925
PB  - Springer
SN  - 00063088
KW  - Bacterial infection
KW  - Histology
KW  - MRSA
KW  - Non-healing wounds
KW  - S. aureus
UR  - https://link.springer.com/article/10.1007/s11756-023-01567-4
N2  - The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.
ER  -

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ, Rea JAROSOVA, Šárka KOBZOVÁ, Lukáš VACEK, Adam NOREK a Lubomír JANDA. A scoring system for histological evaluation of skin during Staphylococcus aureus infections. \textit{Biologia}. New York: Springer, 2024, roč.~79, č.~3, s.~917-925. ISSN~0006-3088. Dostupné z: https://dx.doi.org/10.1007/s11756-023-01567-4.

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