J 2023

Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

BEINHAUEROVÁ, Monika a Iva SLANA

Základní údaje

Originální název

Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Autoři

BEINHAUEROVÁ, Monika a Iva SLANA

Vydání

Journal of Veterinary Research, Sciendo, 2023, 2450-7393

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 1.300

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00132730

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

EID Scopus

Klíčová slova anglicky

Actiphage-qPCR; environmental samples; Mycobacterium avium subsp; paratuberculosis; phage amplification assay; small ruminants

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 3. 1. 2024 10:44, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Introduction: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage. Material and Methods: A total of 9 fresh and 28 frozen (8 or 11 years at -70 degrees C) environmental samples originating from paratuberculosis-affected farms were analysed for the presence of MAP by four different diagnostic methods: Actiphage combined with real-time PCR targeting insertion sequence 900 (IS900 qPCR), conventional phage amplification assay, culture (frozen samples only), and direct IS900 qPCR. Results: Viable MAP was detected in one fresh environmental sample using Actiphage-IS900 qPCR. None of the frozen samples tested positive using this diagnostic approach, which was consistent with the results of culture examination also providing information on viability. Conclusion: This study describes other possible and innovative uses of phage-based methods in paratuberculosis control strategies. The Actiphage-qPCR was found to be less laborious than culture and provided results within six hours, suggesting that it may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination.