Unraveling Epigenetic Changes in A. thaliana Calli: Impact of
HDAC Inhibitors

J 2023

Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors

PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ et. al.

Základní údaje

Originální název

Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors

Autoři

PÍREK, Pavlína (203 Česká republika, domácí), Karolína KRYŠTOFOVÁ (203 Česká republika, domácí), Ingrid KOVÁČOVÁ (703 Slovensko, domácí), Anna KROMEROVÁ (203 Česká republika, domácí), Dagmar ZACHOVÁ (203 Česká republika, domácí), Ondřej HELIA (203 Česká republika, domácí), Klara PANZAROVA (203 Česká republika), Jiří FAJKUS (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Gabriela LOCHMANOVÁ (203 Česká republika, domácí) a Miloslava FOJTOVÁ (203 Česká republika, garant, domácí)

Vydání

PLANTS-BASEL, BASEL, MDPI, 2023, 2223-7747

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.500 v roce 2022

Kód RIV

RIV/00216224:14740/23:00132929

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/plants12244177

UT WoS

001133037600001

Klíčová slova česky

Arabidopsis thaliana; tvorba mozolů; epigenetika; posttranslační modifikace histonů; hmotnostní spektrometrie; trichostatin A; sodium butyrate

Klíčová slova anglicky

Arabidopsis thaliana; callus formation; epigenetics; histone posttranslational modifications; mass spectrometry; trichostatin A; sodium butyrate

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 21:21, Ing. Martina Blahová

Anotace

V originále

The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of Arabidopsis thaliana seedlings. Increased levels of the histone H3.3 variant were found to be the major and most prominent feature of 20-day calli, associated with chromatin relaxation. The methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate, and trichostatin A. Each HDACi affected the state of post-translational histone modifications in a specific manner; NaB-treated calli were epigenetically more similar to root-derived calli, and TSA-treated calli resembled shoot-derived calli.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

GA22-28190S, projekt VaV

Název: Komplexní charakterizace histonových epigenetických značek

Investor: Grantová agentura ČR, Komplexní charakterizace histonových epigenetických značek

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ, Ondřej HELIA, Klara PANZAROVA, Jiří FAJKUS, Zbyněk ZDRÁHAL, Gabriela LOCHMANOVÁ a Miloslava FOJTOVÁ. Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors. PLANTS-BASEL. BASEL: MDPI, 2023, roč. 12, č. 24, s. 1-16. ISSN 2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants12244177.

@article{2359157,
   author = {Pírek, Pavlína and Kryštofová, Karolína and Kováčová, Ingrid and Kromerová, Anna and Zachová, Dagmar and Helia, Ondřej and Panzarova, Klara and Fajkus, Jiří and Zdráhal, Zbyněk and Lochmanová, Gabriela and Fojtová, Miloslava},
   article_location = {BASEL},
   article_number = {24},
   doi = {http://dx.doi.org/10.3390/plants12244177},
   keywords = {Arabidopsis thaliana; callus formation; epigenetics; histone posttranslational modifications; mass spectrometry; trichostatin A; sodium butyrate},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors},
   url = {https://www.mdpi.com/2223-7747/12/24/4177},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2359157
AU  - Pírek, Pavlína - Kryštofová, Karolína - Kováčová, Ingrid - Kromerová, Anna - Zachová, Dagmar - Helia, Ondřej - Panzarova, Klara - Fajkus, Jiří - Zdráhal, Zbyněk - Lochmanová, Gabriela - Fojtová, Miloslava
PY  - 2023
TI  - Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors
JF  - PLANTS-BASEL
VL  - 12
IS  - 24
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 22237747
KW  - Arabidopsis thaliana
KW  - callus formation
KW  - epigenetics
KW  - histone posttranslational modifications
KW  - mass spectrometry
KW  - trichostatin A
KW  - sodium butyrate
UR  - https://www.mdpi.com/2223-7747/12/24/4177
N2  - The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of Arabidopsis thaliana seedlings. Increased levels of the histone H3.3 variant were found to be the major and most prominent feature of 20-day calli, associated with chromatin relaxation. The methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate, and trichostatin A. Each HDACi affected the state of post-translational histone modifications in a specific manner; NaB-treated calli were epigenetically more similar to root-derived calli, and TSA-treated calli resembled shoot-derived calli.
ER  -

PÍREK, Pavlína, Karolína KRYŠTOFOVÁ, Ingrid KOVÁČOVÁ, Anna KROMEROVÁ, Dagmar ZACHOVÁ, Ondřej HELIA, Klara PANZAROVA, Jiří FAJKUS, Zbyněk ZDRÁHAL, Gabriela LOCHMANOVÁ a Miloslava FOJTOVÁ. Unraveling Epigenetic Changes in A. thaliana Calli: Impact of HDAC Inhibitors. \textit{PLANTS-BASEL}. BASEL: MDPI, 2023, roč.~12, č.~24, s.~1-16. ISSN~2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants12244177.

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