Phenotypic characteristics of environmental Pseudomonas
aeruginosa: an in vitro study on epidemiological aspects

J 2023

Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects

GAJDÁCS, Márió; Edit URBÁN; Péter PALLÓS; Adrienn MÁRTA; Danica MATUSOVITS et al.

Základní údaje

Originální název

Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects

Autoři

GAJDÁCS, Márió; Edit URBÁN; Péter PALLÓS; Adrienn MÁRTA; Danica MATUSOVITS; Krisztina KÁRPÁTI; Basem BATTAH; Helal F. HETTA; Ameer KHUSRO; Dani DORDEVIC a Ivan KUSHKEVYCH

Vydání

Acta Biologica Szegediensis, University of Szeged, 2023, 1588-4082

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Maďarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/23:00135361

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.14232/abs.2023.1.35-44

EID Scopus

2-s2.0-85187648807

Klíčová slova anglicky

antimicrobial resistance; biofilm-formation; environmental; MDR; motility; One Health; pigment; Pseudomonas aeruginosa; siderophores

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 1. 2025 17:09, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p > 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p > 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p > 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins.

Návaznosti

MUNI/A/1502/2023, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 4

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 4

Citovat

GAJDÁCS, Márió; Edit URBÁN; Péter PALLÓS; Adrienn MÁRTA; Danica MATUSOVITS; Krisztina KÁRPÁTI; Basem BATTAH; Helal F. HETTA; Ameer KHUSRO; Dani DORDEVIC a Ivan KUSHKEVYCH. Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects. Acta Biologica Szegediensis. University of Szeged, 2023, roč. 67, č. 1, s. 35-44. ISSN 1588-4082. Dostupné z: https://doi.org/10.14232/abs.2023.1.35-44.

@article{2362097,
   author = {Gajdács, Márió and Urbán, Edit and Pallós, Péter and Márta, Adrienn and Matusovits, Danica and Kárpáti, Krisztina and Battah, Basem and Hetta, Helal F. and Khusro, Ameer and Dordevic, Dani and Kushkevych, Ivan},
   article_number = {1},
   doi = {https://doi.org/10.14232/abs.2023.1.35-44},
   keywords = {antimicrobial resistance; biofilm-formation; environmental; MDR; motility; One Health; pigment; Pseudomonas aeruginosa; siderophores},
   language = {eng},
   issn = {1588-4082},
   journal = {Acta Biologica Szegediensis},
   title = {Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects},
   url = {https://abs.bibl.u-szeged.hu/index.php/abs/article/view/3378},
   volume = {67},
   year = {2023}
}

TY  - JOUR
ID  - 2362097
AU  - Gajdács, Márió - Urbán, Edit - Pallós, Péter - Márta, Adrienn - Matusovits, Danica - Kárpáti, Krisztina - Battah, Basem - Hetta, Helal F. - Khusro, Ameer - Dordevic, Dani - Kushkevych, Ivan
PY  - 2023
TI  - Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects
JF  - Acta Biologica Szegediensis
VL  - 67
IS  - 1
SP  - 35-44
EP  - 35-44
PB  - University of Szeged
SN  - 15884082
KW  - antimicrobial resistance
KW  - biofilm-formation
KW  - environmental
KW  - MDR
KW  - motility
KW  - One Health
KW  - pigment
KW  - Pseudomonas aeruginosa
KW  - siderophores
UR  - https://abs.bibl.u-szeged.hu/index.php/abs/article/view/3378
N2  - Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p > 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p > 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p > 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins.
ER  -

GAJDÁCS, Márió; Edit URBÁN; Péter PALLÓS; Adrienn MÁRTA; Danica MATUSOVITS; Krisztina KÁRPÁTI; Basem BATTAH; Helal F. HETTA; Ameer KHUSRO; Dani DORDEVIC a Ivan KUSHKEVYCH. Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects. \textit{Acta Biologica Szegediensis}. University of Szeged, 2023, roč.~67, č.~1, s.~35-44. ISSN~1588-4082. Dostupné z: https://doi.org/10.14232/abs.2023.1.35-44.

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