| MARTINKOVÁ, Petra, Hana KONEČNÁ, Petr GINTAR, Karolína KRYŠTOFOVÁ, David POTĚŠIL, Martin TRTÍLEK a Zbyněk ZDRÁHAL. Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. MDPI, 2023, roč. 24, č. 24, s. "17347", 21 s. ISSN 1661-6596. Dostupné z: https://dx.doi.org/10.3390/ijms242417347. |
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@article{2364078,
author = {Martinková, Petra and Konečná, Hana and Gintar, Petr and Kryštofová, Karolína and Potěšil, David and Trtílek, Martin and Zdráhal, Zbyněk},
article_number = {24},
doi = {http://dx.doi.org/10.3390/ijms242417347},
keywords = {peptide clean-up; magnetic beads; SP2; ethyl acetate extraction; sodium dodecyl sulfate; detergent; polyethylene glycol; Arabidopsis thaliana; LC-MS/MS},
language = {eng},
issn = {1661-6596},
journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
title = {Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS},
url = {https://www.mdpi.com/1422-0067/24/24/17347},
volume = {24},
year = {2023}
}
TY - JOUR
ID - 2364078
AU - Martinková, Petra - Konečná, Hana - Gintar, Petr - Kryštofová, Karolína - Potěšil, David - Trtílek, Martin - Zdráhal, Zbyněk
PY - 2023
TI - Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS
JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
VL - 24
IS - 24
SP - "17347"
EP - "17347"
PB - MDPI
SN - 16616596
KW - peptide clean-up
KW - magnetic beads
KW - SP2
KW - ethyl acetate extraction
KW - sodium dodecyl sulfate
KW - detergent
KW - polyethylene glycol
KW - Arabidopsis thaliana
KW - LC-MS/MS
UR - https://www.mdpi.com/1422-0067/24/24/17347
N2 - The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed SP2) was evaluated for sodium dodecyl sulfate or polyethylene glycol removal from Arabidopsis thaliana tryptic peptides. The robust and efficient 40-min SP2 protocol, tested for 10-ng, 250-ng, and 10-µg peptide samples, was proposed and benchmarked thoroughly against the ethyl acetate extraction protocol. The SP2 protocol on carboxylated magnetic beads proved to be the most robust approach, even for the simultaneous removal of massive sodium dodecyl sulfate (SDS) and polyethylene glycol (PEG) contaminations from AT peptide samples in respect of the LC-MS/MS data outperforming ethyl acetate extraction.
ER -
MARTINKOVÁ, Petra, Hana KONEČNÁ, Petr GINTAR, Karolína KRYŠTOFOVÁ, David POTĚŠIL, Martin TRTÍLEK a Zbyněk ZDRÁHAL. Benchmarking of Two Peptide Clean-Up Protocols: SP2 and Ethyl Acetate Extraction for Sodium Dodecyl Sulfate or Polyethylene Glycol Removal from Plant Samples before LC-MS/MS. \textit{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}. MDPI, 2023, roč.~24, č.~24, s.~''17347'', 21 s. ISSN~1661-6596. Dostupné z: https://dx.doi.org/10.3390/ijms242417347.
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