Identification of Plasmatic microRNA-206 as New Predictor of
Early Recurrence of Atrial Fibrillation after Catheter Ablation
Using Next-generation Sequencing

J 2024

Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing

ŠUSTR, Filip; Táňa MACHÁČKOVÁ; Martin PEŠL; Jana SVAČINOVÁ; Karolína TRACHTOVÁ et. al.

Základní údaje

Originální název

Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing

Autoři

ŠUSTR, Filip; Táňa MACHÁČKOVÁ; Martin PEŠL; Jana SVAČINOVÁ; Karolína TRACHTOVÁ; Zdeněk STÁREK; Bohuslav KIANIČKA; Ondřej SLABÝ a Jan NOVÁK

Vydání

MOLECULAR DIAGNOSIS & THERAPY, AUCKLAND, ADIS INT LTD, 2024, 1177-1062

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Nový Zéland

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.400

Kód RIV

RIV/00216224:14110/24:00135395

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1007/s40291-024-00698-x

UT WoS

001177382100001

EID Scopus

2-s2.0-85186885685

Klíčová slova anglicky

Plasmatic MicroRNA-206; Atrial Fibrillation; Catheter Ablation

Štítky

14110115, 14110116, 14110513, 14110515, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 9. 2025 07:19, Mgr. Tereza Miškechová

Anotace

V originále

Background Catheter ablation (CA) of atrial fibrillation (AF) is indicated in patients with recurrent and symptomatic AF episodes. Despite the strict inclusion/exclusion criteria, AF recurrence after CA remains high. Identification of a novel biomarker that would predict AF recurrence would help to stratify the patients. The aim of the study was to seek novel biomarkers among the plasmatic microRNAs (miRNAs, miRs). Methods A prospective monocentric study was conducted. A total of 49 consecutive AF patients indicated for CA were included. Blood sampling was performed prior to CA. RNA was isolated from plasma using commercial kits. In the exploration phase, small RNA sequencing was performed in ten AF patients (five with and five without AF recurrence) using Illumina instrument. In the validation phase, levels of selected miRNAs were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in all participants. Results Altogether, 22 miRNAs were identified as altered between the groups by next-generation sequencing (using the DESeq2 algorithm). Using qRT-PCR, levels of the five most altered miRNAs (miR-190b/206/326/505-5p/1296-5p) were verified in the whole cohort. Plasma levels of hsa-miR-206 were significantly higher in patients with early (within 6 months) AF recurrence and showed an increase of risk recurrence,2.65 times by every increase in its level by 1 unit in the binary logistic regression. Conclusion We have identified a set of 22 plasmatic miRNAs that differ between the patients with and without AF recurrence after CA and confirmed hsa-miR-206 as a novel miRNA associated with early AF recurrence. Results shall be verified in a larger independent cohort.

Návaznosti

LX22NPO5104, projekt VaV

Název: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Akronym: CarDia)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut pro léčbu metabolických a kardiovaskulárních onemocnění, 5.1 EXCELES

MUNI/A/1156/2021, interní kód MU

Název: Diferenciální diagnostika a odhad prognózy vnitřních nemocí 5 (Akronym: DIDIPRON 5)

Investor: Masarykova univerzita, Diferenciální diagnostika a odhad prognózy vnitřních nemocí 5

MUNI/A/1377/2022, interní kód MU

Název: Diferenciální diagnostika a odhad prognózy vnitřních nemocí 6

Investor: Masarykova univerzita, Diferenciální diagnostika a odhad prognózy vnitřních nemocí 6

MUNI/A/1555/2023, interní kód MU

Název: Patofyziologie, epidemiologie, diferenciální diagnostika a odhad prognózy vybraných vnitřních nemocí

Investor: Masarykova univerzita, Patofyziologie, epidemiologie, diferenciální diagnostika a odhad prognózy vybraných vnitřních nemocí

Přiložené soubory

Identification_of_Plasmatic_MicroRNA-206_as_New_Predictor_of_Early_Recurrence_of_Atrial_Fibrillation_After_Catheter_Ablation_Using_Next-generation_Sequencing.pdf

Citovat

ŠUSTR, Filip; Táňa MACHÁČKOVÁ; Martin PEŠL; Jana SVAČINOVÁ; Karolína TRACHTOVÁ; Zdeněk STÁREK; Bohuslav KIANIČKA; Ondřej SLABÝ a Jan NOVÁK. Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing. MOLECULAR DIAGNOSIS & THERAPY. AUCKLAND: ADIS INT LTD, 2024, roč. 28, č. 3, s. 301-310. ISSN 1177-1062. Dostupné z: https://doi.org/10.1007/s40291-024-00698-x.

@article{2365441,
   author = {Šustr, Filip and Macháčková, Táňa and Pešl, Martin and Svačinová, Jana and Trachtová, Karolína and Stárek, Zdeněk and Kianička, Bohuslav and Slabý, Ondřej and Novák, Jan},
   article_location = {AUCKLAND},
   article_number = {3},
   doi = {https://doi.org/10.1007/s40291-024-00698-x},
   keywords = {Plasmatic MicroRNA-206; Atrial Fibrillation; Catheter Ablation},
   language = {eng},
   issn = {1177-1062},
   journal = {MOLECULAR DIAGNOSIS & THERAPY},
   title = {Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing},
   url = {https://link.springer.com/article/10.1007/s40291-024-00698-x},
   volume = {28},
   year = {2024}
}

TY  - JOUR
ID  - 2365441
AU  - Šustr, Filip - Macháčková, Táňa - Pešl, Martin - Svačinová, Jana - Trachtová, Karolína - Stárek, Zdeněk - Kianička, Bohuslav - Slabý, Ondřej - Novák, Jan
PY  - 2024
TI  - Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing
JF  - MOLECULAR DIAGNOSIS & THERAPY
VL  - 28
IS  - 3
SP  - 301-310
EP  - 301-310
PB  - ADIS INT LTD
SN  - 11771062
KW  - Plasmatic MicroRNA-206
KW  - Atrial Fibrillation
KW  - Catheter Ablation
UR  - https://link.springer.com/article/10.1007/s40291-024-00698-x
N2  - Background Catheter ablation (CA) of atrial fibrillation (AF) is indicated in patients with recurrent and symptomatic AF episodes. Despite the strict inclusion/exclusion criteria, AF recurrence after CA remains high. Identification of a novel biomarker that would predict AF recurrence would help to stratify the patients. The aim of the study was to seek novel biomarkers among the plasmatic microRNAs (miRNAs, miRs). Methods A prospective monocentric study was conducted. A total of 49 consecutive AF patients indicated for CA were included. Blood sampling was performed prior to CA. RNA was isolated from plasma using commercial kits. In the exploration phase, small RNA sequencing was performed in ten AF patients (five with and five without AF recurrence) using Illumina instrument. In the validation phase, levels of selected miRNAs were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in all participants. Results Altogether, 22 miRNAs were identified as altered between the groups by next-generation sequencing (using the DESeq2 algorithm). Using qRT-PCR, levels of the five most altered miRNAs (miR-190b/206/326/505-5p/1296-5p) were verified in the whole cohort. Plasma levels of hsa-miR-206 were significantly higher in patients with early (within 6 months) AF recurrence and showed an increase of risk recurrence,2.65 times by every increase in its level by 1 unit in the binary logistic regression. Conclusion We have identified a set of 22 plasmatic miRNAs that differ between the patients with and without AF recurrence after CA and confirmed hsa-miR-206 as a novel miRNA associated with early AF recurrence. Results shall be verified in a larger independent cohort.
ER  -

ŠUSTR, Filip; Táňa MACHÁČKOVÁ; Martin PEŠL; Jana SVAČINOVÁ; Karolína TRACHTOVÁ; Zdeněk STÁREK; Bohuslav KIANIČKA; Ondřej SLABÝ a Jan NOVÁK. Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing. \textit{MOLECULAR DIAGNOSIS \&{} THERAPY}. AUCKLAND: ADIS INT LTD, 2024, roč.~28, č.~3, s.~301-310. ISSN~1177-1062. Dostupné z: https://doi.org/10.1007/s40291-024-00698-x.

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