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@article{2365441,
author = {Šustr, Filip and Macháčková, Táňa and Pešl, Martin and Svačinová, Jana and Trachtová, Karolína and Stárek, Zdeněk and Kianička, Bohuslav and Slabý, Ondřej and Novák, Jan},
article_location = {AUCKLAND},
doi = {http://dx.doi.org/10.1007/s40291-024-00698-x},
keywords = {Plasmatic MicroRNA-206; Atrial Fibrillation; Catheter Ablation},
language = {eng},
issn = {1177-1062},
journal = {MOLECULAR DIAGNOSIS & THERAPY},
title = {Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing},
url = {https://link.springer.com/article/10.1007/s40291-024-00698-x},
year = {2024}
}
TY - JOUR
ID - 2365441
AU - Šustr, Filip - Macháčková, Táňa - Pešl, Martin - Svačinová, Jana - Trachtová, Karolína - Stárek, Zdeněk - Kianička, Bohuslav - Slabý, Ondřej - Novák, Jan
PY - 2024
TI - Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing
JF - MOLECULAR DIAGNOSIS & THERAPY
PB - ADIS INT LTD
SN - 11771062
KW - Plasmatic MicroRNA-206
KW - Atrial Fibrillation
KW - Catheter Ablation
UR - https://link.springer.com/article/10.1007/s40291-024-00698-x
N2 - Background Catheter ablation (CA) of atrial fibrillation (AF) is indicated in patients with recurrent and symptomatic AF episodes. Despite the strict inclusion/exclusion criteria, AF recurrence after CA remains high. Identification of a novel biomarker that would predict AF recurrence would help to stratify the patients. The aim of the study was to seek novel biomarkers among the plasmatic microRNAs (miRNAs, miRs). Methods A prospective monocentric study was conducted. A total of 49 consecutive AF patients indicated for CA were included. Blood sampling was performed prior to CA. RNA was isolated from plasma using commercial kits. In the exploration phase, small RNA sequencing was performed in ten AF patients (five with and five without AF recurrence) using Illumina instrument. In the validation phase, levels of selected miRNAs were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in all participants. Results Altogether, 22 miRNAs were identified as altered between the groups by next-generation sequencing (using the DESeq2 algorithm). Using qRT-PCR, levels of the five most altered miRNAs (miR-190b/206/326/505-5p/1296-5p) were verified in the whole cohort. Plasma levels of hsa-miR-206 were significantly higher in patients with early (within 6 months) AF recurrence and showed an increase of risk recurrence,2.65 times by every increase in its level by 1 unit in the binary logistic regression. Conclusion We have identified a set of 22 plasmatic miRNAs that differ between the patients with and without AF recurrence after CA and confirmed hsa-miR-206 as a novel miRNA associated with early AF recurrence. Results shall be verified in a larger independent cohort.
ER -
ŠUSTR, Filip, Táňa MACHÁČKOVÁ, Martin PEŠL, Jana SVAČINOVÁ, Karolína TRACHTOVÁ, Zdeněk STÁREK, Bohuslav KIANIČKA, Ondřej SLABÝ a Jan NOVÁK. Identification of Plasmatic microRNA-206 as New Predictor of Early Recurrence of Atrial Fibrillation after Catheter Ablation Using Next-generation Sequencing. \textit{MOLECULAR DIAGNOSIS \&{} THERAPY}. AUCKLAND: ADIS INT LTD, 2024. ISSN~1177-1062. Dostupné z: https://dx.doi.org/10.1007/s40291-024-00698-x.
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