Preparative agarose gel electrophoresis for reducing matrix
interferences of organoid cell medium prior to LC-MS analysis
of insulin

J 2024

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

HRUŠKOVÁ, Helena; Christine OLSEN; Roman ŘEMÍNEK; Chencheng WANG; Aleksandra AIZENSHTADT et al.

Základní údaje

Originální název

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Autoři

HRUŠKOVÁ, Helena; Christine OLSEN; Roman ŘEMÍNEK; Chencheng WANG; Aleksandra AIZENSHTADT; Stefan KRAUSS; Hanne SCHOLZ; Hanne RØBERG-LARSEN; František FORET a Steven Ray WILSON

Vydání

Journal of Chromatography A, Elsevier B.V. 2024, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00135448

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2024 12:07, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.

Citovat

HRUŠKOVÁ, Helena; Christine OLSEN; Roman ŘEMÍNEK; Chencheng WANG; Aleksandra AIZENSHTADT; Stefan KRAUSS; Hanne SCHOLZ; Hanne RØBERG-LARSEN; František FORET a Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. Journal of Chromatography A. Elsevier B.V., 2024, roč. 1717, February, s. 1-7. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2024.464669.

@article{2369020,
   author = {Hrušková, Helena and Olsen, Christine and Řemínek, Roman and Wang, Chencheng and Aizenshtadt, Aleksandra and Krauss, Stefan and Scholz, Hanne and RøbergandLarsen, Hanne and Foret, František and Wilson, Steven Ray},
   article_number = {February},
   doi = {https://doi.org/10.1016/j.chroma.2024.464669},
   keywords = {Liquid chromatography-mass spectrometry; Insulin; Preparative gel electrophoresis; Stem cell-derived islet organoids},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967324000426},
   volume = {1717},
   year = {2024}
}

TY  - JOUR
ID  - 2369020
AU  - Hrušková, Helena - Olsen, Christine - Řemínek, Roman - Wang, Chencheng - Aizenshtadt, Aleksandra - Krauss, Stefan - Scholz, Hanne - Røberg-Larsen, Hanne - Foret, František - Wilson, Steven Ray
PY  - 2024
TI  - Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
JF  - Journal of Chromatography A
VL  - 1717
IS  - February
SP  - 1-7
EP  - 1-7
PB  - Elsevier B.V.
SN  - 00219673
KW  - Liquid chromatography-mass spectrometry
KW  - Insulin
KW  - Preparative gel electrophoresis
KW  - Stem cell-derived islet organoids
UR  - https://www.sciencedirect.com/science/article/pii/S0021967324000426
N2  - Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.
ER  -

HRUŠKOVÁ, Helena; Christine OLSEN; Roman ŘEMÍNEK; Chencheng WANG; Aleksandra AIZENSHTADT; Stefan KRAUSS; Hanne SCHOLZ; Hanne RØBERG-LARSEN; František FORET a Steven Ray WILSON. Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin. \textit{Journal of Chromatography A}. Elsevier B.V., 2024, roč.~1717, February, s.~1-7. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2024.464669.

Přehled o publikaci