Impedimetric immunosensor for microalbuminuria based on a
WS2/Au water-phase assembled nanocomposite

J 2023

Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE, Petr SKLÁDAL et. al.

Základní údaje

Originální název

Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite

Autoři

SILVERI, Filippo (380 Itálie, domácí), Radka OBOŘILOVÁ (203 Česká republika, domácí), Jakub MÁČALA (203 Česká republika, domácí), Dario COMPAGNONE a Petr SKLÁDAL (203 Česká republika, domácí)

Vydání

Microchimica Acta, Wien, Springer, 2023, 0026-3672

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.700 v roce 2022

Kód RIV

RIV/00216224:14310/23:00133461

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00604-023-05873-1

UT WoS

001033463400005

Klíčová slova anglicky

Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion

Štítky

CF CRYO, CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 10. 2024 14:00, Ing. Jana Kuchtová

Anotace

V originále

An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.

Návaznosti

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE a Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. Microchimica Acta. Wien: Springer, 2023, roč. 190, č. 8, s. 1-12. ISSN 0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-023-05873-1.

@article{2370317,
   author = {Silveri, Filippo and Obořilová, Radka and Máčala, Jakub and Compagnone, Dario and Skládal, Petr},
   article_location = {Wien},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s00604-023-05873-1},
   keywords = {Human serum albumin; Impedimetric sensor; Transition metal dichalcogenides; Nanocomposite; Aqueous-based dispersion},
   language = {eng},
   issn = {0026-3672},
   journal = {Microchimica Acta},
   title = {Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite},
   url = {https://link.springer.com/article/10.1007/s00604-023-05873-1},
   volume = {190},
   year = {2023}
}

TY  - JOUR
ID  - 2370317
AU  - Silveri, Filippo - Obořilová, Radka - Máčala, Jakub - Compagnone, Dario - Skládal, Petr
PY  - 2023
TI  - Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite
JF  - Microchimica Acta
VL  - 190
IS  - 8
SP  - 1-12
EP  - 1-12
PB  - Springer
SN  - 00263672
KW  - Human serum albumin
KW  - Impedimetric sensor
KW  - Transition metal dichalcogenides
KW  - Nanocomposite
KW  - Aqueous-based dispersion
UR  - https://link.springer.com/article/10.1007/s00604-023-05873-1
N2  - An electrochemical impedimetric biosensor for human serum albumin (HSA) determination is proposed. The biosensor is based on water-phase assembled nanocomposites made of 2D WS2 nanoflakes and Au nanoparticles (AuNPs). The WS2 has been produced using a liquid-phase exfoliation strategy assisted by sodium cholate, obtaining a water-stable suspension that allowed the straightforward decoration with AuNPs directly in the aqueous phase. The resulting WS2/Au nanocomposite has been characterized by atomic force microscopy and Raman spectroscopy and, then, employed to modify screen-printed electrodes. Good electron-transfer features have been achieved. An electrochemical immunosensing platform has been assembled exploiting cysteamine- glutaraldehyde covalent chemistry for antibody (Ab) immobilization. The resulting immunosensor exhibited good sensitivity for HSA detection (LOD = 2 ng mL(-1)), with extended linear range (0.005 - 100 mu g mL(-1)), providing a useful analytical tool for HSA determination in urine at relevant clinical ranges for microalbuminuria screening. The HSA quantification in human urine samples resulted in recoveries from 91.8 to 112.4% and was also reproducible (RSD < 7.5%, n = 3), with marked selectivity. This nanocomposite, thanks to the reliable performance and the ease of the assembling strategy, is a promising alternative for electrochemical immunosensing of health relevant markers.
ER  -

SILVERI, Filippo, Radka OBOŘILOVÁ, Jakub MÁČALA, Dario COMPAGNONE a Petr SKLÁDAL. Impedimetric immunosensor for microalbuminuria based on a WS2/Au water-phase assembled nanocomposite. \textit{Microchimica Acta}. Wien: Springer, 2023, roč.~190, č.~8, s.~1-12. ISSN~0026-3672. Dostupné z: https://dx.doi.org/10.1007/s00604-023-05873-1.

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