Challenges of in situ hybridization in miRNA analysis of
triple-negative breast cancer morphological diversity

J 2023

Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

KOLECKOVA, Marketa, Michala BEZDEKOVA, Jan BOUCHAL, Maria JANIKOVA, Júlia BOHOŠOVÁ et. al.

Základní údaje

Originální název

Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity

Autoři

KOLECKOVA, Marketa, Michala BEZDEKOVA, Jan BOUCHAL, Maria JANIKOVA, Júlia BOHOŠOVÁ (703 Slovensko, domácí), Ondřej SLABÝ (203 Česká republika, domácí), Marek SVOBODA (203 Česká republika, domácí), Josef SROVNAL, Katherine VOMACKOVA, Ivo UBERALL a Zdenek KOLAR (garant)

Vydání

Neoplasma, Bratislava, Slovenská akademie vied, 2023, 0028-2685

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.000 v roce 2022

Kód RIV

RIV/00216224:14740/23:00133481

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.4149/neo_2023_220519N533

UT WoS

001070933100001

Klíčová slova anglicky

triple-negative breast cancer (TNBC); miRNA; in situ hybridization; RT-qPCR

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 3. 2024 22:29, Mgr. Eva Dubská

Anotace

V originále

miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint. However, it has not been considered that miRNA expression profile may be associated with a specific morphological entity inside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, where we confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatory infiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detection compared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.

Citovat

KOLECKOVA, Marketa, Michala BEZDEKOVA, Jan BOUCHAL, Maria JANIKOVA, Júlia BOHOŠOVÁ, Ondřej SLABÝ, Marek SVOBODA, Josef SROVNAL, Katherine VOMACKOVA, Ivo UBERALL a Zdenek KOLAR. Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity. Neoplasma. Bratislava: Slovenská akademie vied, 2023, roč. 70, č. 2, s. 179-187. ISSN 0028-2685. Dostupné z: https://dx.doi.org/10.4149/neo_2023_220519N533.

@article{2371140,
 author = {Koleckova, Marketa and Bezdekova, Michala and Bouchal, Jan and Janikova, Maria and Bohošová, Júlia and Slabý, Ondřej and Svoboda, Marek and Srovnal, Josef and Vomackova, Katherine and Uberall, Ivo and Kolar, Zdenek},
 article_location = {Bratislava},
 article_number = {2},
 doi = {http://dx.doi.org/10.4149/neo_2023_220519N533},
 keywords = {triple-negative breast cancer (TNBC); miRNA; in situ hybridization; RT-qPCR},
 language = {eng},
 issn = {0028-2685},
 journal = {Neoplasma},
 title = {Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity},
 url = {http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7984&category_id=189&option=com_virtuemart&vmcchk=1&Itemid=1},
 volume = {70},
 year = {2023}
}

TY - JOUR
ID - 2371140
AU - Koleckova, Marketa - Bezdekova, Michala - Bouchal, Jan - Janikova, Maria - Bohošová, Júlia - Slabý, Ondřej - Svoboda, Marek - Srovnal, Josef - Vomackova, Katherine - Uberall, Ivo - Kolar, Zdenek
PY - 2023
TI - Challenges of in situ hybridization in miRNA analysis of triple-negative breast cancer morphological diversity
JF - Neoplasma
VL - 70
IS - 2
SP - 179-187
EP - 179-187
PB - Slovenská akademie vied
SN - 00282685
KW - triple-negative breast cancer (TNBC)
KW - miRNA
KW - in situ hybridization
KW - RT-qPCR
UR - http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7984&category_id=189&option=com_virtuemart&vmcchk=1&Itemid=1
N2 - miRNA expression in triple-negative breast cancers (TNBC) has mainly been studied from a methodological viewpoint. However, it has not been considered that miRNA expression profile may be associated with a specific morphological entity inside every tumor. The verification of this hypothesis on a set of 25 TNBCs was the subject of our previous work, where we confirmed specific expression of the studied miRNAs in 82 samples of different morphologies including inflammatory infiltrate, spindle cell, clear cell, and metastases after RNA extraction and purification as well as microchip and biostatistical analysis. In the current work, we demonstrate a low suitability of in situ hybridization method for miRNA detection compared to RT-qPCR, and in detail discuss the biological role of 8 miRNAs with the most significant changes of expression.
ER -

KOLECKOVA, Marketa, Michala BEZDEKOVA, Jan BOUCHAL, Maria JANIKOVA, Júlia BOHOŠOVÁ, Ondřej SLABÝ, Marek SVOBODA, Josef SROVNAL, Katherine VOMACKOVA, Ivo UBERALL a Zdenek KOLAR. Challenges of \&{}lt;i\&{}gt;in situ\&{}lt;/i\&{}gt; hybridization in miRNA analysis of triple-negative breast cancer morphological diversity. \textit{Neoplasma}. Bratislava: Slovenská akademie vied, 2023, roč.~70, č.~2, s.~179-187. ISSN~0028-2685. Dostupné z: https://dx.doi.org/10.4149/neo\_{}2023\_{}220519N533.

Podrobný výpis o publikaci

Challenges of <i>in situ</i> hybridization in miRNA analysis of triple-negative breast cancer morphological diversity