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@article{2372118, author = {Luo, Yu and Lenarčič Živkovic, Martina and Wang, Jiawei and Ryneš, Jan and Trantírková, Silvie and Trantírek, Lukáš and Verga, Daniela and Mergny, JeanandLouis}, article_location = {Oxford}, article_number = {1}, doi = {http://dx.doi.org/10.1093/nar/gkad1073}, keywords = {G-QUADRUPLEX; BASE-PAIR; DNA; STABILITY; metal ions; G/C rich motifs; NMR; PROMOTER}, language = {eng}, issn = {0305-1048}, journal = {Nucleic acids research}, title = {A sodium/potassium switch for G4-prone G/C-rich sequences}, url = {https://academic.oup.com/nar/article/52/1/448/7438919?login=true}, volume = {52}, year = {2024} }
TY - JOUR ID - 2372118 AU - Luo, Yu - Lenarčič Živkovic, Martina - Wang, Jiawei - Ryneš, Jan - Trantírková, Silvie - Trantírek, Lukáš - Verga, Daniela - Mergny, Jean-Louis PY - 2024 TI - A sodium/potassium switch for G4-prone G/C-rich sequences JF - Nucleic acids research VL - 52 IS - 1 SP - 448-461 EP - 448-461 PB - Oxford University Press SN - 03051048 KW - G-QUADRUPLEX KW - BASE-PAIR KW - DNA KW - STABILITY KW - metal ions KW - G/C rich motifs KW - NMR KW - PROMOTER UR - https://academic.oup.com/nar/article/52/1/448/7438919?login=true N2 - Metal ions are essential components for the survival of living organisms. For most species, intracellular and extracellular ionic conditions differ significantly. As G-quadruplexes (G4s) are ion-dependent structures, changes in the [Na+]/[K+] ratio may affect the folding of genomic G4s. More than 11000 putative G4 sequences in the human genome (hg19) contain at least two runs of three continuous cytosines, and these mixed G/C-rich sequences may form a quadruplex or a competing hairpin structure based on G-C base pairing. In this study, we examine how the [Na+]/[K+] ratio influences the structures of G/C-rich sequences. The natural G4 structure with a 9-nt long central loop, CEBwt, was chosen as a model sequence, and the loop bases were gradually replaced by cytosines. The series of CEB mutations revealed that the presence of cytosines in G4 loops does not prevent G4 folding or decrease G4 stability but increases the probability of forming a competing structure, either a hairpin or an intermolecular duplex. Slow conversion to the quadruplex in vitro (in a potassium-rich buffer) and cells was demonstrated by NMR. 'Shape-shifting' sequences may respond to [Na+]/[K+] changes with delayed kinetics. ER -
LUO, Yu, Martina LENARČIČ ŽIVKOVIC, Jiawei WANG, Jan RYNEŠ, Silvie TRANTÍRKOVÁ, Lukáš TRANTÍREK, Daniela VERGA a Jean-Louis MERGNY. A sodium/potassium switch for G4-prone G/C-rich sequences. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2024, roč.~52, č.~1, s.~448-461. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkad1073.
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