Production and characterization of two-domain lectins from
Photorhabdus spp.

k 2023

Production and characterization of two-domain lectins from Photorhabdus spp.

BURÁŇOVÁ, Tereza, Eva PAULENOVÁ a Michaela WIMMEROVÁ

Základní údaje

Originální název

Production and characterization of two-domain lectins from Photorhabdus spp.

Název česky

Produkce a charakterizace dvoudoménových lektinů z Photorhabdus spp.

Autoři

BURÁŇOVÁ, Tereza (203 Česká republika, domácí), Eva PAULENOVÁ (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant)

Vydání

XXII. Setkání biochemiků a molekulárních biologů, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/23:00133678

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0409-5

Klíčová slova česky

lektiny; Photorhabdus laumondii; molekulární klonování; produkce rekombinantního proteinu

Klíčová slova anglicky

lectins; Photorhabdus laumondii; molecular cloning; recombinant protein production

Změněno: 28. 2. 2024 16:27, Mgr. Tereza Buráňová

Anotace

V originále

Lectins are proteins that non-covalently and reversibly bind saccharides. They are ubiquitous, found in organisms of all kingdoms, and interact, for example, with carbohydrate groups found on the surface of cells. Bacteria use the properties of lectins to recognize host cells and attach to them. Bacterial infection cannot occur without prior adhesion, and this is where lectins play an important role. Genes encoding lectins have been found in the genomes of various bacteria, including Photorhabdus spp. Photorhabdus spp. are Gram-negative bacteria that combine mutualistic and pathogenic processes during their life cycle. They live in a mutualistic relationship with nematodes Heterorhabditis spp. with which they form a pathogenic complex. Together they infect mostly insects, though some species are also human opportunistic pathogens. In this research, we focus on the characterization of a group of two-domain lectins from P. laumondii. Basic bioinformatic analysis was used to predict the properties of these proteins. One of the proteins, named PpxD, was selected for further characterization. Cloning and production of the recombinant protein was performed. The binding properties of the PpxD protein were characterized by the batch method and hemagglutination assay.

Návaznosti

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

Citovat

BURÁŇOVÁ, Tereza, Eva PAULENOVÁ a Michaela WIMMEROVÁ. Production and characterization of two-domain lectins from Photorhabdus spp. In XXII. Setkání biochemiků a molekulárních biologů. 2023. ISBN 978-80-280-0409-5.

@proceedings{2379657,
   author = {Buráňová, Tereza and Paulenová, Eva and Wimmerová, Michaela},
   booktitle = {XXII. Setkání biochemiků a molekulárních biologů},
   keywords = {lectins; Photorhabdus laumondii; molecular cloning; recombinant protein production},
   language = {eng},
   isbn = {978-80-280-0409-5},
   title = {Production and characterization of two-domain lectins from Photorhabdus spp.},
   year = {2023}
}

TY  - CONF
ID  - 2379657
AU  - Buráňová, Tereza - Paulenová, Eva - Wimmerová, Michaela
PY  - 2023
TI  - Production and characterization of two-domain lectins from Photorhabdus spp.
SN  - 9788028004095
KW  - lectins
KW  - Photorhabdus laumondii
KW  - molecular cloning
KW  - recombinant protein production
N2  - Lectins are proteins that non-covalently and reversibly bind saccharides. They are ubiquitous, found in organisms of all kingdoms, and interact, for example, with carbohydrate groups found on the surface of cells. Bacteria use the properties of lectins to recognize host cells and attach to them. Bacterial infection cannot occur without prior adhesion, and this is where lectins play an important role. Genes encoding lectins have been found in the genomes of various bacteria, including Photorhabdus spp. Photorhabdus spp. are Gram-negative bacteria that combine mutualistic and pathogenic processes during their life cycle. They live in a mutualistic relationship with nematodes Heterorhabditis spp. with which they form a pathogenic complex. Together they infect mostly insects, though some species are also human opportunistic pathogens. In this research, we focus on the characterization of a group of two-domain lectins from P. laumondii. Basic bioinformatic analysis was used to predict the properties of these proteins. One of the proteins, named PpxD, was selected for further characterization. Cloning and production of the recombinant protein was performed. The binding properties of the PpxD protein were characterized by the batch method and hemagglutination assay.
ER  -

BURÁŇOVÁ, Tereza, Eva PAULENOVÁ a Michaela WIMMEROVÁ. Production and characterization of two-domain lectins from Photorhabdus spp. In \textit{XXII. Setkání biochemiků a molekulárních biologů}. 2023. ISBN~978-80-280-0409-5.

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