Global interactome mapping reveals pro-tumorigenic interactions
of NF-κB in breast cancer

J 2024

Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER et al.

Základní údaje

Originální název

Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer

Autoři

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL

Vydání

Molecular and Cellular Proteomics, Elsevier, 2024, 1535-9484

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.500

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00135622

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

NF-κB; RELA; Proteomics; Protein complexes; Interaction; Breast cancer; Protein correlation profiling; AlphaPullDown

Štítky

14313050, 143160, CF PROT, NÚVR_CEITEC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2025 00:54, Mgr. Eva Dubská

Anotace

V originále

NF-κB pathway is involved in inflammation, however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size exclusion chromatography (SEC) fractions identifies 5460 protein groups. 7568 interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation and DNA replication. Central NF-κB transcription regulator RELA co-elutes with interactors of NF-κB activator PRMT5, and these complexes are confirmed by AlphaPulldown prediction. A complementary immunoprecipitation experiment recapitulates RELA interactions with other NF-κB factors, associating NF-κB inhibition with lower binding of NF-κB activators to RELA. This study describes a network of pro-tumorigenic protein interactions and their rearrangement upon NF-κB inhibition with potential therapeutic implications in tumors with high NF-κB activity.

Návaznosti

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

NU22-08-00230, projekt VaV

Název: Proteogenomová klasifikace trojitě negativních nádorů prsu ve vztahu k prognóze a cílené terapii

Investor: Ministerstvo zdravotnictví ČR, Proteogenomová klasifikace trojitě negativních nádorů prsu ve vztahu k prognóze a cílené terapii, Podprogram 1 - standardní

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Přiložené soubory

2381418.pdf

Citovat

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL. Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer. Molecular and Cellular Proteomics. Elsevier, 2024, roč. 23, č. 4, s. 1-20. ISSN 1535-9484. Dostupné z: https://doi.org/10.1016/j.mcpro.2024.100744.

@article{2381418,
   author = {Lapčík, Petr and Stacey, R. Greg and Potěšil, David and Kulhánek, Petr and Foster, Leonard J. and Bouchal, Pavel},
   article_number = {4},
   doi = {https://doi.org/10.1016/j.mcpro.2024.100744},
   keywords = {NF-κB; RELA; Proteomics; Protein complexes; Interaction; Breast cancer; Protein correlation profiling; AlphaPullDown},
   language = {eng},
   issn = {1535-9484},
   journal = {Molecular and Cellular Proteomics},
   title = {Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer},
   url = {https://www.sciencedirect.com/science/article/pii/S1535947624000343},
   volume = {23},
   year = {2024}
}

TY  - JOUR
ID  - 2381418
AU  - Lapčík, Petr - Stacey, R. Greg - Potěšil, David - Kulhánek, Petr - Foster, Leonard J. - Bouchal, Pavel
PY  - 2024
TI  - Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer
JF  - Molecular and Cellular Proteomics
VL  - 23
IS  - 4
SP  - 1-20
EP  - 1-20
PB  - Elsevier
SN  - 15359484
KW  - NF-κB
KW  - RELA
KW  - Proteomics
KW  - Protein complexes
KW  - Interaction
KW  - Breast cancer
KW  - Protein correlation profiling
KW  - AlphaPullDown
UR  - https://www.sciencedirect.com/science/article/pii/S1535947624000343
N2  - NF-κB pathway is involved in inflammation, however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size exclusion chromatography (SEC) fractions identifies 5460 protein groups. 7568 interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation and DNA replication. Central NF-κB transcription regulator RELA co-elutes with interactors of NF-κB activator PRMT5, and these complexes are confirmed by AlphaPulldown prediction. A complementary immunoprecipitation experiment recapitulates RELA interactions with other NF-κB factors, associating NF-κB inhibition with lower binding of NF-κB activators to RELA. This study describes a network of pro-tumorigenic protein interactions and their rearrangement upon NF-κB inhibition with potential therapeutic implications in tumors with high NF-κB activity.
ER  -

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL. Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer. \textit{Molecular and Cellular Proteomics}. Elsevier, 2024, roč.~23, č.~4, s.~1-20. ISSN~1535-9484. Dostupné z: https://doi.org/10.1016/j.mcpro.2024.100744.

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