Comparison of microbial communities and the profile of
sulfate-reducing bacteria in patients with ulcerative colitis
and their association with bowel diseases: a pilot study

J 2024

Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study

KUSHKEVYCH, Ivan, Kristýna MARTÍNKOVÁ, Lenka MRÁKOVÁ, Francesco GIUDICI, Simone BALDI et. al.

Základní údaje

Originální název

Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study

Autoři

KUSHKEVYCH, Ivan (203 Česká republika, garant, domácí), Kristýna MARTÍNKOVÁ (203 Česká republika, domácí), Lenka MRÁKOVÁ (203 Česká republika, domácí), Francesco GIUDICI, Simone BALDI, David NOVÁK (203 Česká republika, domácí), Márió GAJDÁCS, Monika VÍTĚZOVÁ (203 Česká republika, domácí), Dani DORDEVIC, Amedeo AMEDEI a Simon K.-M. R. RITTMANN

Vydání

Microbial Cell, Shared Science Publishers OG, 2024, 2311-2638

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.600 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.15698/mic2024.03.817

UT WoS

001187090800001

Klíčová slova anglicky

gut microbiota; ulcerative colitis; gut dysbiosis; sulfate-reducing bacteria; inflammatory bowel disease; 16S rRNA gene sequencing

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2024 09:46, Mgr. Marie Šípková, DiS.

Anotace

V originále

Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition – focusing on sulfate-reducing bacteria (SRB) – in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients’ disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases.

Návaznosti

MUNI/A/1280/2022, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 3

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 3

Citovat

KUSHKEVYCH, Ivan, Kristýna MARTÍNKOVÁ, Lenka MRÁKOVÁ, Francesco GIUDICI, Simone BALDI, David NOVÁK, Márió GAJDÁCS, Monika VÍTĚZOVÁ, Dani DORDEVIC, Amedeo AMEDEI a Simon K.-M. R. RITTMANN. Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study. Microbial Cell. Shared Science Publishers OG, 2024, roč. 11, č. 1, s. 79-89. ISSN 2311-2638. Dostupné z: https://dx.doi.org/10.15698/mic2024.03.817.

@article{2385498,
   author = {Kushkevych, Ivan and Martínková, Kristýna and Mráková, Lenka and Giudici, Francesco and Baldi, Simone and Novák, David and Gajdács, Márió and Vítězová, Monika and Dordevic, Dani and Amedei, Amedeo and Rittmann, Simon K.andM. R.},
   article_number = {1},
   doi = {http://dx.doi.org/10.15698/mic2024.03.817},
   keywords = {gut microbiota; ulcerative colitis; gut dysbiosis; sulfate-reducing bacteria; inflammatory bowel disease; 16S rRNA gene sequencing},
   language = {eng},
   issn = {2311-2638},
   journal = {Microbial Cell},
   title = {Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study},
   url = {https://microbialcell.com/researcharticles/2024a-kushkevych-microbial-cell/},
   volume = {11},
   year = {2024}
}

TY  - JOUR
ID  - 2385498
AU  - Kushkevych, Ivan - Martínková, Kristýna - Mráková, Lenka - Giudici, Francesco - Baldi, Simone - Novák, David - Gajdács, Márió - Vítězová, Monika - Dordevic, Dani - Amedei, Amedeo - Rittmann, Simon K.-M. R.
PY  - 2024
TI  - Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study
JF  - Microbial Cell
VL  - 11
IS  - 1
SP  - 79-89
EP  - 79-89
PB  - Shared Science Publishers OG
SN  - 23112638
KW  - gut microbiota
KW  - ulcerative colitis
KW  - gut dysbiosis
KW  - sulfate-reducing bacteria
KW  - inflammatory bowel disease
KW  - 16S rRNA gene sequencing
UR  - https://microbialcell.com/researcharticles/2024a-kushkevych-microbial-cell/
N2  - Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition – focusing on sulfate-reducing bacteria (SRB) – in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients’ disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases.
ER  -

KUSHKEVYCH, Ivan, Kristýna MARTÍNKOVÁ, Lenka MRÁKOVÁ, Francesco GIUDICI, Simone BALDI, David NOVÁK, Márió GAJDÁCS, Monika VÍTĚZOVÁ, Dani DORDEVIC, Amedeo AMEDEI a Simon K.-M. R. RITTMANN. Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study. \textit{Microbial Cell}. Shared Science Publishers OG, 2024, roč.~11, č.~1, s.~79-89. ISSN~2311-2638. Dostupné z: https://dx.doi.org/10.15698/mic2024.03.817.

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