First insight into strongylid nematode diversity and
anthelmintic treatment effectiveness in beef cattle in the
Czech Republic explored by HTS metagenomics

J 2024

First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics

PAFČO, Barbora; Eva NOSKOVÁ; Vladislav ILÍK; Lucia ANETTOVÁ; Barbora CERVENA et al.

Základní údaje

Originální název

First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics

Autoři

Vydání

VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, NETHERLANDS, ELSEVIER, 2024, 2405-9390

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30310 Parasitology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00135707

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cattle; Strongylid nematodes; Resistance; Anthelmintic treatment

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2026 10:34, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Parasitic diseases and mitigation of their effects play an important role in the health management of grazing livestock worldwide, with gastrointestinal strongylid nematodes being of prominent importance. These helminths typically occur in complex communities, often composed of species from numerous strongylid genera. Detecting the full diversity of strongylid species in non-invasively collected faecal samples is nearly impossible using conventional methods. In contrast, high-throughput amplicon sequencing (HTS) can effectively identify co-occurring species. During the four-year project, we collected and analysed faecal samples from beef cattle on >120 farms throughout the Czech Republic. Strongylids were the predominant nematodes, detected in 56% of the samples, but at a low level of infection. The apparent limitations in identifying strongylid taxa prompted this pilot study on a representative group of samples testing positive for strongylids using ITS-2 metabarcoding. The most widespread genera parasitizing Czech cattle were Ostertagia (O. ostertagi) and Oesophagostomum spp., followed by Trichostrongylus and Cooperia, while Bunostomum, Nematodirus and Chabertia were present only in a minority. As comparative material, 21 samples of cattle from the Danube Delta in Romania were used, which, in contrast, were dominated by Haemonchus placei. Finally, the effect of ivermectin treatment was tested at two Czech farms. After treatment with the anthelmintic, there was a shift in the strongylid communities, with a dominance of Cooperia and Ostertagia.

Návaznosti

MUNI/A/1422/2022, interní kód MU

Název: Ekologické faktory a evoluční mechanismy determinující strukturu a funkci biotických společenstev a symbiotických systémů

Investor: Masarykova univerzita, Ekologické faktory a evoluční mechanismy determinující strukturu a funkci biotických společenstev a symbiotických systémů

90132, velká výzkumná infrastruktura

Název: NCMG II

90140, velká výzkumná infrastruktura

Název: e-INFRA CZ

Citovat

PAFČO, Barbora; Eva NOSKOVÁ; Vladislav ILÍK; Lucia ANETTOVÁ; Barbora CERVENA; Jakub KREISINGER; Ilona PSENKOVA; Petr VACLAVEK; Tereza VYHLIDALOVA; Jana JEZKOVA; Kamil MALAT; Andrei D MIHALCA a David MODRÝ. First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS. NETHERLANDS: ELSEVIER, 2024, roč. 47, January, s. "100961", 7 s. ISSN 2405-9390. Dostupné z: https://doi.org/10.1016/j.vprsr.2023.100961.

@article{2386715,
   author = {Pafčo, Barbora and Nosková, Eva and Ilík, Vladislav and Anettová, Lucia and Cervena, Barbora and Kreisinger, Jakub and Psenkova, Ilona and Vaclavek, Petr and Vyhlidalova, Tereza and Jezkova, Jana and Malat, Kamil and Mihalca, Andrei D and Modrý, David},
   article_location = {NETHERLANDS},
   article_number = {January},
   doi = {https://doi.org/10.1016/j.vprsr.2023.100961},
   keywords = {Cattle; Strongylid nematodes; Resistance; Anthelmintic treatment},
   language = {eng},
   issn = {2405-9390},
   journal = {VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS},
   title = {First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics},
   url = {https://doi.org/10.1016/j.vprsr.2023.100961},
   volume = {47},
   year = {2024}
}

TY  - JOUR
ID  - 2386715
AU  - Pafčo, Barbora - Nosková, Eva - Ilík, Vladislav - Anettová, Lucia - Cervena, Barbora - Kreisinger, Jakub - Psenkova, Ilona - Vaclavek, Petr - Vyhlidalova, Tereza - Jezkova, Jana - Malat, Kamil - Mihalca, Andrei D - Modrý, David
PY  - 2024
TI  - First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics
JF  - VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
VL  - 47
IS  - January
SP  - "100961"
EP  - "100961"
PB  - ELSEVIER
SN  - 24059390
KW  - Cattle
KW  - Strongylid nematodes
KW  - Resistance
KW  - Anthelmintic treatment
UR  - https://doi.org/10.1016/j.vprsr.2023.100961
N2  - Parasitic diseases and mitigation of their effects play an important role in the health management of grazing livestock worldwide, with gastrointestinal strongylid nematodes being of prominent importance. These helminths typically occur in complex communities, often composed of species from numerous strongylid genera. Detecting the full diversity of strongylid species in non-invasively collected faecal samples is nearly impossible using conventional methods. In contrast, high-throughput amplicon sequencing (HTS) can effectively identify co-occurring species. During the four-year project, we collected and analysed faecal samples from beef cattle on >120 farms throughout the Czech Republic. Strongylids were the predominant nematodes, detected in 56% of the samples, but at a low level of infection. The apparent limitations in identifying strongylid taxa prompted this pilot study on a representative group of samples testing positive for strongylids using ITS-2 metabarcoding. The most widespread genera parasitizing Czech cattle were Ostertagia (O. ostertagi) and Oesophagostomum spp., followed by Trichostrongylus and Cooperia, while Bunostomum, Nematodirus and Chabertia were present only in a minority. As comparative material, 21 samples of cattle from the Danube Delta in Romania were used, which, in contrast, were dominated by Haemonchus placei. Finally, the effect of ivermectin treatment was tested at two Czech farms. After treatment with the anthelmintic, there was a shift in the strongylid communities, with a dominance of Cooperia and Ostertagia.
ER  -

PAFČO, Barbora; Eva NOSKOVÁ; Vladislav ILÍK; Lucia ANETTOVÁ; Barbora CERVENA; Jakub KREISINGER; Ilona PSENKOVA; Petr VACLAVEK; Tereza VYHLIDALOVA; Jana JEZKOVA; Kamil MALAT; Andrei D MIHALCA a David MODRÝ. First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics. \textit{VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS}. NETHERLANDS: ELSEVIER, 2024, roč.~47, January, s.~''100961'', 7 s. ISSN~2405-9390. Dostupné z: https://doi.org/10.1016/j.vprsr.2023.100961.

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