Cyanobacterial bloom-associated lipopolysaccharides induce
pro-inflammatory processes in keratinocytes in vitro

J 2024

Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro

RAPTOVÁ, Petra; Veronika SKOČKOVÁ; Pavel BABICA; Iva SOVADINOVÁ; Eliška SYCHROVÁ et al.

Základní údaje

Originální název

Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro

Autoři

RAPTOVÁ, Petra; Veronika SKOČKOVÁ; Pavel BABICA; Iva SOVADINOVÁ ; Eliška SYCHROVÁ ; Petra VÍDEŇSKÁ; Petra ŠPLÍCHALOVÁ; O. VASICEK a L. SINDLEROVA

Vydání

Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2024, 1382-6689

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00135727

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cyanobacterial harmful algae bloom; lipopolysaccharide; Axenic cyanobacteria; Inflammation; Human HaCaT keratinocytes; Genomic analyses

Štítky

14314010, 143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 2. 2025 10:22, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Our previous studies have shown that CyanoHAB LPS (lipopolysaccharides) and LPS from cyanobacterial cultures induce pro-inflammatory effects on intestinal epithelial and immune cells in vitro. To expand our understanding, we investigated their impact on human keratinocytes, which are targeted during water recreational activities. LPS samples were isolated from CyanoHAB biomasses dominated by Microcystis, Aphanizomenon, Planktothrix, and Dolichospermum, or from axenic cultures of these genera. We identified two CyanoHAB biomasses containing a high proportion of Gram-negative bacteria, including potentially pathogenic genera. These biomasses showed the highest induction of interleukin (IL) 8, IL-6, C-C motif chemokine ligand (CCL) 2 (also known as MCP-1), and CCL20 production by HaCaT cells. Interestingly, all CyanoHAB-derived LPS and LPS from axenic cultures (except for Microcystis) accelerated cell proliferation and migration. Our findings highlight the role of G- bacteria composition and LPS structural disparities in influencing these effects, with implications for skin health during recreational activities.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GA19-09980S, projekt VaV

Název: Prozánětlivé působení LPS sinicového vodního květu z vodních nádrží České republiky

Investor: Grantová agentura ČR, Prozánětlivé působení LPS sinicového vodního květu z rekreačních nádrží České republiky

90269, velká výzkumná infrastruktura

Název: RECETOX RI II

Citovat

RAPTOVÁ, Petra; Veronika SKOČKOVÁ; Pavel BABICA; Iva SOVADINOVÁ; Eliška SYCHROVÁ; Petra VÍDEŇSKÁ; Petra ŠPLÍCHALOVÁ; O. VASICEK a L. SINDLEROVA. Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro. Environmental Toxicology and Pharmacology. Amsterdam: Elsevier Science B.V., 2024, roč. 105, January 2024, s. 1-13. ISSN 1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2023.104342.

@article{2387639,
   author = {Raptová, Petra and Skočková, Veronika and Babica, Pavel and Sovadinová, Iva and Sychrová, Eliška and Vídeňská, Petra and Šplíchalová, Petra and Vasicek, O. and Sindlerova, L.},
   article_location = {Amsterdam},
   article_number = {January 2024},
   doi = {https://doi.org/10.1016/j.etap.2023.104342},
   keywords = {Cyanobacterial harmful algae bloom; lipopolysaccharide; Axenic cyanobacteria; Inflammation; Human HaCaT keratinocytes; Genomic analyses},
   language = {eng},
   issn = {1382-6689},
   journal = {Environmental Toxicology and Pharmacology},
   title = {Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro},
   url = {https://www.sciencedirect.com/science/article/pii/S1382668923002843?via%3Dihub},
   volume = {105},
   year = {2024}
}

TY  - JOUR
ID  - 2387639
AU  - Raptová, Petra - Skočková, Veronika - Babica, Pavel - Sovadinová, Iva - Sychrová, Eliška - Vídeňská, Petra - Šplíchalová, Petra - Vasicek, O. - Sindlerova, L.
PY  - 2024
TI  - Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro
JF  - Environmental Toxicology and Pharmacology
VL  - 105
IS  - January 2024
SP  - 1-13
EP  - 1-13
PB  - Elsevier Science B.V.
SN  - 13826689
KW  - Cyanobacterial harmful algae bloom
KW  - lipopolysaccharide
KW  - Axenic cyanobacteria
KW  - Inflammation
KW  - Human HaCaT keratinocytes
KW  - Genomic analyses
UR  - https://www.sciencedirect.com/science/article/pii/S1382668923002843?via%3Dihub
N2  - Our previous studies have shown that CyanoHAB LPS (lipopolysaccharides) and LPS from cyanobacterial cultures induce pro-inflammatory effects on intestinal epithelial and immune cells in vitro. To expand our understanding, we investigated their impact on human keratinocytes, which are targeted during water recreational activities. LPS samples were isolated from CyanoHAB biomasses dominated by Microcystis, Aphanizomenon, Planktothrix, and Dolichospermum, or from axenic cultures of these genera. We identified two CyanoHAB biomasses containing a high proportion of Gram-negative bacteria, including potentially pathogenic genera. These biomasses showed the highest induction of interleukin (IL) 8, IL-6, C-C motif chemokine ligand (CCL) 2 (also known as MCP-1), and CCL20 production by HaCaT cells. Interestingly, all CyanoHAB-derived LPS and LPS from axenic cultures (except for Microcystis) accelerated cell proliferation and migration. Our findings highlight the role of G- bacteria composition and LPS structural disparities in influencing these effects, with implications for skin health during recreational activities.
ER  -

RAPTOVÁ, Petra; Veronika SKOČKOVÁ; Pavel BABICA; Iva SOVADINOVÁ; Eliška SYCHROVÁ; Petra VÍDEŇSKÁ; Petra ŠPLÍCHALOVÁ; O. VASICEK a L. SINDLEROVA. Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro. \textit{Environmental Toxicology and Pharmacology}. Amsterdam: Elsevier Science B.V., 2024, roč.~105, January 2024, s.~1-13. ISSN~1382-6689. Dostupné z: https://doi.org/10.1016/j.etap.2023.104342.

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