Induction of somatic embryogenesis and ectopic proliferation in
Tecoma stans (L.) Juss. ex Kunth cell suspension culture

J 2024

Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture

OMAR, Rowida; Ivan KUSHKEVYCH a Mohamed Abd EL-SALAM

Základní údaje

Originální název

Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture

Autoři

OMAR, Rowida; Ivan KUSHKEVYCH a Mohamed Abd EL-SALAM

Vydání

In Vitro Cellular & Developmental Biology - Plant, Springer, 2024, 1054-5476

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.900

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00135836

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Tecoma stans; Somatic embryogenesis; Plant cell culture; Plant regeneration; Organogenesis

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 1. 2025 13:20, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important in vitro techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from Tecoma stans (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L−1 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L−1) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the specified hormonal conditions, leading to subsequent development into the mature vegetative phase after an additional 4 wk. Significant embryo production (16 ± 2.0 torpedo stage embryos per 50 mL culture media) was observed in Murashige and Skoog medium enriched with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid and 2.0 mg L−1 6-benzyladenine, surpassing the results observed with other concentrations (p-value < 0.0001). The generated somatic embryos can serve as a potential in vitro tool for the propagation, generation, and organogenesis of T. stans, contributing to its role as both an ornamental and medicinal plant. Moreover, the induction of somatic embryogenesis opens avenues for the potential production of T. stans bioactive secondary metabolites and diverse applications in biotechnology, biotransformation, and biocatalysis, particularly in the conversion of both exogenous and endogenous substrates, such as tecomine—the principal antidiabetic alkaloid in the leaf extract.

Návaznosti

MUNI/A/1502/2023, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 4

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 4

Citovat

OMAR, Rowida; Ivan KUSHKEVYCH a Mohamed Abd EL-SALAM. Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture. In Vitro Cellular & Developmental Biology - Plant. Springer, 2024, roč. 60, č. 3, s. 396-402. ISSN 1054-5476. Dostupné z: https://doi.org/10.1007/s11627-024-10421-4.

@article{2395097,
   author = {Omar, Rowida and Kushkevych, Ivan and ElandSalam, Mohamed Abd},
   article_number = {3},
   doi = {https://doi.org/10.1007/s11627-024-10421-4},
   keywords = {Tecoma stans; Somatic embryogenesis; Plant cell culture; Plant regeneration; Organogenesis},
   language = {eng},
   issn = {1054-5476},
   journal = {In Vitro Cellular & Developmental Biology - Plant},
   title = {Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture},
   url = {https://link.springer.com/article/10.1007/s11627-024-10421-4},
   volume = {60},
   year = {2024}
}

TY  - JOUR
ID  - 2395097
AU  - Omar, Rowida - Kushkevych, Ivan - El-Salam, Mohamed Abd
PY  - 2024
TI  - Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture
JF  - In Vitro Cellular & Developmental Biology - Plant
VL  - 60
IS  - 3
SP  - 396-402
EP  - 396-402
PB  - Springer
SN  - 10545476
KW  - Tecoma stans
KW  - Somatic embryogenesis
KW  - Plant cell culture
KW  - Plant regeneration
KW  - Organogenesis
UR  - https://link.springer.com/article/10.1007/s11627-024-10421-4
N2  - Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important in vitro techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from Tecoma stans (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L−1 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L−1) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the specified hormonal conditions, leading to subsequent development into the mature vegetative phase after an additional 4 wk. Significant embryo production (16 ± 2.0 torpedo stage embryos per 50 mL culture media) was observed in Murashige and Skoog medium enriched with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid and 2.0 mg L−1 6-benzyladenine, surpassing the results observed with other concentrations (p-value < 0.0001). The generated somatic embryos can serve as a potential in vitro tool for the propagation, generation, and organogenesis of T. stans, contributing to its role as both an ornamental and medicinal plant. Moreover, the induction of somatic embryogenesis opens avenues for the potential production of T. stans bioactive secondary metabolites and diverse applications in biotechnology, biotransformation, and biocatalysis, particularly in the conversion of both exogenous and endogenous substrates, such as tecomine—the principal antidiabetic alkaloid in the leaf extract.
ER  -

OMAR, Rowida; Ivan KUSHKEVYCH a Mohamed Abd EL-SALAM. Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture. \textit{In Vitro Cellular \&{} Developmental Biology - Plant}. Springer, 2024, roč.~60, č.~3, s.~396-402. ISSN~1054-5476. Dostupné z: https://doi.org/10.1007/s11627-024-10421-4.

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