2024
Understanding excreted/secreted products in Heterorhabditis bacteriophora: Activation Material Insights
ŠREIBR, Sara, Jana ILGOVÁ, Pavel DOBEŠ, Jacek MARCINIAK, Jiří VOREL et. al.Základní údaje
Originální název
Understanding excreted/secreted products in Heterorhabditis bacteriophora: Activation Material Insights
Autoři
ŠREIBR, Sara (276 Německo, garant, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Jacek MARCINIAK (203 Česká republika, domácí), Jiří VOREL (203 Česká republika, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Martin KAŠNÝ (203 Česká republika, domácí) a Pavel HYRŠL (203 Česká republika, domácí)
Vydání
35th Symposium of the European Society of Nematologists, 2024
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10616 Entomology
Stát vydavatele
Španělsko
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Excreted/secreted products; Heterorhabditis bacteriophora; activation
Změněno: 2. 5. 2024 23:25, doc. RNDr. Pavel Hyršl, Ph.D.
V originále
Entomopathogenic nematodes (EPNs) are insect parasites commonly used in the biological control of various insect pests. EPNs release biologically active compounds known as excreted/secreted products (ESPs) that play a crucial role in interaction with the host's immune system. This interaction enhances infection efficiency, thereby increasing the chances of EPNs' survival and successful reproduction. Unravelling the composition and function of ESPs is essential for understanding the mechanisms of nematode pathogenicity and optimizing their effectiveness as biocontrol agents. In this study, we aimed to evaluate the protein spectrum of Heterorhabditis bacteriophora ESPs released in relation of different activation materials. We identified the infection-specific molecules by comparing ESP profiles between infective juveniles treated with materials prepared from insects and controls. We optimized the in vitro activation process of H. bacteriophora and selected three fractions of activation materials from Galleria mellonella larvae, each prepared differently, along with two control fractions: water or phosphate buffer. We collected ESPs from all treatments and subjected them to LC-MS/MS analysis. For protein identification, we conducted searches against a protein database originating from the H. bacteriophora reference genome. The results recorded using the LC-MS/MS analysis revealed significant differences in the composition of ESPs based on the materials used for nematodes’ in vitro activation. Within these ESPs, we identified numerous proteins with potential to play significant role in nematode-insect interaction; including serpin domain-containing protein, metalloendopeptidase, ShTK domain-containing proteins and fatty acid- retinoid- binding proteins. Furthermore, our findings indicate that the choice of activation materials significantly influences both the abundance and diversity of collected ESP. This implies that the way of nematode activation may play a crucial role in modulating the virulence of the EPNs, suggesting an increase in the effectiveness of biocontrol. This study was supported by grant GAČR 23-06457S.
Česky
Entomopathogenic nematodes (EPNs) are insect parasites commonly used in the biological control of various insect pests. EPNs release biologically active compounds known as excreted/secreted products (ESPs) that play a crucial role in interaction with the host's immune system. This interaction enhances infection efficiency, thereby increasing the chances of EPNs' survival and successful reproduction. Unravelling the composition and function of ESPs is essential for understanding the mechanisms of nematode pathogenicity and optimizing their effectiveness as biocontrol agents. In this study, we aimed to evaluate the protein spectrum of Heterorhabditis bacteriophora ESPs released in relation of different activation materials. We identified the infection-specific molecules by comparing ESP profiles between infective juveniles treated with materials prepared from insects and controls. We optimized the in vitro activation process of H. bacteriophora and selected three fractions of activation materials from Galleria mellonella larvae, each prepared differently, along with two control fractions: water or phosphate buffer. We collected ESPs from all treatments and subjected them to LC-MS/MS analysis. For protein identification, we conducted searches against a protein database originating from the H. bacteriophora reference genome. The results recorded using the LC-MS/MS analysis revealed significant differences in the composition of ESPs based on the materials used for nematodes’ in vitro activation. Within these ESPs, we identified numerous proteins with potential to play significant role in nematode-insect interaction; including serpin domain-containing protein, metalloendopeptidase, ShTK domain-containing proteins and fatty acid- retinoid- binding proteins. Furthermore, our findings indicate that the choice of activation materials significantly influences both the abundance and diversity of collected ESP. This implies that the way of nematode activation may play a crucial role in modulating the virulence of the EPNs, suggesting an increase in the effectiveness of biocontrol. This study was supported by grant GAČR 23-06457S.
Návaznosti
| GA23-06457S, projekt VaV |
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