Quality Control of Human Pluripotent Stem Cell-Derived Retinal
Pigment Epithelium Cells for Regenerative Therapy

a 2024

Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy

SOURALOVÁ, Tereza; Katarína ČIMBOROVÁ; Vendula PELKOVÁ; Aleš HAMPL; Irena KOUTNÁ et al.

Základní údaje

Originální název

Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy

Autoři

SOURALOVÁ, Tereza; Katarína ČIMBOROVÁ; Vendula PELKOVÁ; Aleš HAMPL a Irena KOUTNÁ

Vydání

XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/24:00135897

Organizační jednotka

Lékařská fakulta

ISBN

978-80-01-07293-6

Klíčová slova anglicky

Quality control; Human pluripotent stem cells; Retinal pigment epithelium

Příznaky

Mezinárodní význam

Změněno: 17. 1. 2025 09:32, Mgr. Tereza Miškechová

Anotace

V originále

Human retinal pigment epithelial (RPE) cells form a monolayer of polarized pigmented epithelial cells located between the blood vessels of the eye and the neural retina. The key function of RPE cells is the maintenance and protection of light-sensitive photoreceptors. Degeneration of RPE cells accompanies age-related eye diseases and can lead to the loss of vision. The rescue of the patient´s vision can be achieved by the replacement of the RPE cells. Therefore, we aim to differentiate human embryonal stem cells and human induced pluripotent stem cells into fully functional RPE cells that would be suitable for regenerative therapy. To ensure the RPE cells are not released for the treatment of the patient until the quality has been considered satisfactory, we designed a panel of quality control tests that characterize a) functionality: phagocytosis, specific cytokine secretion, b) morphology, c) identity and viability: marker detection by immunocytochemistry and flowcytometry, d) immunogenicity: lymphocyte-graft cell immune reaction, HLA expression of RPE cells. These tests are prepared for translation into advanced therapy medicinal product (ATMP) manufacture according to good manufacturing practice (GMP).

Návaznosti

LM2023049, projekt VaV

Název: Český národní uzel Evropské sítě infrastruktur klinického výzkumu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CZECRIN - Czech National Node to the European Clinical Research Infrastructure Network

MUNI/A/1598/2023, interní kód MU

Název: Zdroje pro tkáňové inženýrství 14

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 14

NU22-08-00629, projekt VaV

Název: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk

Investor: Ministerstvo zdravotnictví ČR, Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk, Podprogram 1 - standardní

Citovat

SOURALOVÁ, Tereza; Katarína ČIMBOROVÁ; Vendula PELKOVÁ; Aleš HAMPL a Irena KOUTNÁ. Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy. In XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice. 2024. ISBN 978-80-01-07293-6.

@proceedings{2397977,
   author = {Souralová, Tereza and Čimborová, Katarína and Pelková, Vendula and Hampl, Aleš and Koutná, Irena},
   booktitle = {XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice},
   keywords = {Quality control; Human pluripotent stem cells; Retinal pigment epithelium},
   language = {eng},
   isbn = {978-80-01-07293-6},
   title = {Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy},
   year = {2024}
}

TY  - CONF
ID  - 2397977
AU  - Souralová, Tereza - Čimborová, Katarína - Pelková, Vendula - Hampl, Aleš - Koutná, Irena
PY  - 2024
TI  - Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy
SN  - 9788001072936
KW  - Quality control
KW  - Human pluripotent stem cells
KW  - Retinal pigment epithelium
N2  - Human retinal pigment epithelial (RPE) cells form a monolayer of polarized pigmented epithelial cells located between the blood vessels of the eye and the neural retina. The key function of RPE cells is the maintenance and protection of light-sensitive photoreceptors. Degeneration of RPE cells accompanies age-related eye diseases and can lead to the loss of vision. The rescue of the patient´s vision can be achieved by the replacement of the RPE cells. Therefore, we aim to differentiate human embryonal stem cells and human induced pluripotent stem cells into fully functional RPE cells that would be suitable for regenerative therapy. To ensure the RPE cells are not released for the treatment of the patient until the quality has been considered satisfactory, we designed a panel of quality control tests that characterize a) functionality: phagocytosis, specific cytokine secretion, b) morphology, c) identity and viability: marker detection by immunocytochemistry and flowcytometry, d) immunogenicity: lymphocyte-graft cell immune reaction, HLA expression of RPE cells. These tests are prepared for translation into advanced therapy medicinal product (ATMP) manufacture according to good manufacturing practice (GMP).
ER  -

SOURALOVÁ, Tereza; Katarína ČIMBOROVÁ; Vendula PELKOVÁ; Aleš HAMPL a Irena KOUTNÁ. Quality Control of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells for Regenerative Therapy. In \textit{XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice}. 2024. ISBN~978-80-01-07293-6.

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