Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous
Chondrocytes in Biodegradable Hydrogels

a 2024

Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels

DLUGOŠOVÁ, Slavomíra, Jana DVOŘÁKOVÁ, Vladimír PROKS a Irena KOUTNÁ

Základní údaje

Originální název

Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels

Autoři

DLUGOŠOVÁ, Slavomíra, Jana DVOŘÁKOVÁ, Vladimír PROKS a Irena KOUTNÁ

Vydání

XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice, 2024. 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Příznaky

Mezinárodní význam

Změněno: 3. 5. 2024 15:24, Mgr. Slavomíra Dlugošová

Anotace

V originále

Regeneration of hyaline cartilage is a challenging endeavor due to its limited regenerative capacity. The challenge arises from the tendency of chondrocytes to dedifferentiate into fibroblast-like cells, compromising their ability to maintain a stable phenotype for effective tissue engineering applications. This project is focused on the 3D culture of human articular chondrocytes within biodegradable hydrogel* while investigating the feasibility and efficacy of this technique. The methodology involves the encapsulation of human articular chondrocytes within biodegradable hydrogels, optimizing culture conditions to facilitate cell proliferation, differentiation, and extracellular matrix production. Immunocytochemical analysis on cryosections allows the detection of the synthetic activity of chondrocytes. The results obtained so far show that under 3D conditions, significantly higher production of type II collagen (specific for chondrocytes) was detected while 2D control was dominated by type I collagen (fibroblasts). The hydrogel environment thus effectively enables the maintenance of the chondrocyte phenotype. Chondrocytes maintain high viability and proliferative activity during 3D culturing. The prospects of this project extend to clinical applications in hyaline cartilage tissue engineering, offering a promising solution for repairing cartilage defects. This research aims to contribute and ultimately improve patient outcomes in orthopedic surgery and restoring joint function.

Návaznosti

LM2023049, projekt VaV

Název: Český národní uzel Evropské sítě infrastruktur klinického výzkumu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CZECRIN - Czech National Node to the European Clinical Research Infrastructure Network

LM2023050, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech BioImaging: Národní výzkumná infrastruktura pro biologické a medicínské zobrazování

MUNI/A/1598/2023, interní kód MU

Název: Zdroje pro tkáňové inženýrství 14

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 14

Citovat

DLUGOŠOVÁ, Slavomíra, Jana DVOŘÁKOVÁ, Vladimír PROKS a Irena KOUTNÁ. Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels. In XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice, 2024. 2024.

@proceedings{2398899,
   author = {Dlugošová, Slavomíra and Dvořáková, Jana and Proks, Vladimír and Koutná, Irena},
   booktitle = {XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice, 2024.},
   language = {eng},
   title = {Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels},
   year = {2024}
}

TY  - CONF
ID  - 2398899
AU  - Dlugošová, Slavomíra - Dvořáková, Jana - Proks, Vladimír - Koutná, Irena
PY  - 2024
TI  - Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels
N2  - Regeneration of hyaline cartilage is a challenging endeavor due to its limited regenerative capacity. The challenge arises from the tendency of chondrocytes to dedifferentiate into fibroblast-like cells, compromising their ability to maintain a stable phenotype for effective tissue engineering applications. This project is focused on the 3D culture of human articular chondrocytes within biodegradable hydrogel* while investigating the feasibility and efficacy of this technique. The methodology involves the encapsulation of human articular chondrocytes within biodegradable hydrogels, optimizing culture conditions to facilitate cell proliferation, differentiation, and extracellular matrix production. Immunocytochemical analysis on cryosections allows the detection of the synthetic activity of chondrocytes. The results obtained so far show that under 3D conditions, significantly higher production of type II collagen (specific for chondrocytes) was detected while 2D control was dominated by type I collagen (fibroblasts). The hydrogel environment thus effectively enables the maintenance of the chondrocyte phenotype. Chondrocytes maintain high viability and proliferative activity during 3D culturing. The prospects of this project extend to clinical applications in hyaline cartilage tissue engineering, offering a promising solution for repairing cartilage defects. This research aims to contribute and ultimately improve patient outcomes in orthopedic surgery and restoring joint function.
ER  -

DLUGOŠOVÁ, Slavomíra, Jana DVOŘÁKOVÁ, Vladimír PROKS a Irena KOUTNÁ. Hyaline Cartilage Tissue Engineering: 3D Culture of Autologous Chondrocytes in Biodegradable Hydrogels. In \textit{XV. mezinárodní konferenci Bioimplantologie a regenerativní medicína, Lednice, 2024.}. 2024.

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