Faecal egg count reduction test in goats: Zooming in on the
genus level

J 2024

Faecal egg count reduction test in goats: Zooming in on the genus level

MAURIZIO, Anna; Lucie ŠKORPÍKOVÁ; Jana ILGOVÁ; Cinzia TESSARIN; Giorgia DOTTO et al.

Základní údaje

Originální název

Faecal egg count reduction test in goats: Zooming in on the genus level

Autoři

MAURIZIO, Anna; Lucie ŠKORPÍKOVÁ; Jana ILGOVÁ; Cinzia TESSARIN; Giorgia DOTTO; Nikol RESLOVÁ; Jaroslav VADLEJCH; Erica MARCHIORI; di Regalbono Antonio FRANGIPANE; Martin KAŠNÝ a Rudi CASSINI

Vydání

Veterinary Parasitology, AMSTERDAM, Elsevier Science, 2024, 0304-4017

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00139819

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Gastrointestinal tract; Caprine nematodes; Species richness; Coprology; QPCR; Anthelmintic resistance

Štítky

14314020, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 1. 2025 14:21, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only qualitative or semi-quantitative. The updated WAAVP guidelines now recommend assessing anthelmintic efficacy for each GIN genus/species separately (genus-specific FECRT), but this approach is poorly employed in Europe and in goats especially. For this reason, four FECRT trials were conducted using oxfendazole and eprinomectin in two Italian goat farms. Samples were processed individually using the McMaster technique and then pooled to create two samples from faeces of 5 animals each. Pooled samples were analysed using the McMaster and cultured for seven days at 26(degrees)C to obtain L3s. The genus-specific FECRT was based on larval identification, integrating coproculture and FEC results. Larvae were identified as Haemonchus, Trichostrongylus, Teladorsagia, Oesophagostomum / Chabertia and Bunostomum. Molecular assays (a multiplex real-time PCR and two end-point PCRs) were also implemented on pooled samples to support the morphological identification. The Spearmann Rho test confirmed a high correlation between the two approaches (Rho = 0.941 and Rho = 0.914 respectively for Haemonchus and Trichostrongylus, the two most common genera). Both oxfendazole and eprinomectin were effective in one farm, while none in the other farm (FECR = 75.9% and 73.3% respectively). In the second farm, the genus-specific FECRT highlighted a different response to treatment among genera: oxfendazole lacked efficacy against both Haemonchus and Trichostrongylus spp., eprinomectin only against Haemonchus, while all other genera were susceptible to both drugs. This study brings new attention on the importance of adopting a genus-specific approach to identify and quantify differences in susceptibility to anthelmintics among genera in goats, providing support for FECRT interpretation, anthelmintic resistance evaluation and evidence-based GIN control.

Návaznosti

SS05010070, projekt VaV

Název: Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Investor: Technologická agentura ČR, Šíření invazních druhů parazitů a jejich drtivé dopady na biologickou rozmanitost původních druhů přežvýkavců

Citovat

MAURIZIO, Anna; Lucie ŠKORPÍKOVÁ; Jana ILGOVÁ; Cinzia TESSARIN; Giorgia DOTTO; Nikol RESLOVÁ; Jaroslav VADLEJCH; Erica MARCHIORI; di Regalbono Antonio FRANGIPANE; Martin KAŠNÝ a Rudi CASSINI. Faecal egg count reduction test in goats: Zooming in on the genus level. Veterinary Parasitology. AMSTERDAM: Elsevier Science, 2024, roč. 327, April, s. 1-8. ISSN 0304-4017. Dostupné z: https://doi.org/10.1016/j.vetpar.2024.110146.

@article{2399418,
   author = {Maurizio, Anna and Škorpíková, Lucie and Ilgová, Jana and Tessarin, Cinzia and Dotto, Giorgia and Reslová, Nikol and Vadlejch, Jaroslav and Marchiori, Erica and Frangipane, di Regalbono Antonio and Kašný, Martin and Cassini, Rudi},
   article_location = {AMSTERDAM},
   article_number = {April},
   doi = {https://doi.org/10.1016/j.vetpar.2024.110146},
   keywords = {Gastrointestinal tract; Caprine nematodes; Species richness; Coprology; QPCR; Anthelmintic resistance},
   language = {eng},
   issn = {0304-4017},
   journal = {Veterinary Parasitology},
   title = {Faecal egg count reduction test in goats: Zooming in on the genus level},
   url = {https://doi.org/10.1016/j.vetpar.2024.110146},
   volume = {327},
   year = {2024}
}

TY  - JOUR
ID  - 2399418
AU  - Maurizio, Anna - Škorpíková, Lucie - Ilgová, Jana - Tessarin, Cinzia - Dotto, Giorgia - Reslová, Nikol - Vadlejch, Jaroslav - Marchiori, Erica - Frangipane, di Regalbono Antonio - Kašný, Martin - Cassini, Rudi
PY  - 2024
TI  - Faecal egg count reduction test in goats: Zooming in on the genus level
JF  - Veterinary Parasitology
VL  - 327
IS  - April
SP  - 1-8
EP  - 1-8
PB  - Elsevier Science
SN  - 03044017
KW  - Gastrointestinal tract
KW  - Caprine nematodes
KW  - Species richness
KW  - Coprology
KW  - QPCR
KW  - Anthelmintic resistance
UR  - https://doi.org/10.1016/j.vetpar.2024.110146
N2  - The faecal egg count reduction test (FECRT) is the most widely used method to assess treatment efficacy against gastrointestinal nematodes (GIN). Information on genera composition of the GIN community is not available with this test and it is commonly obtained by identifying cultured third-stage larvae (L3) or through molecular assays in the post-treatment survey, but results provided are usually only qualitative or semi-quantitative. The updated WAAVP guidelines now recommend assessing anthelmintic efficacy for each GIN genus/species separately (genus-specific FECRT), but this approach is poorly employed in Europe and in goats especially. For this reason, four FECRT trials were conducted using oxfendazole and eprinomectin in two Italian goat farms. Samples were processed individually using the McMaster technique and then pooled to create two samples from faeces of 5 animals each. Pooled samples were analysed using the McMaster and cultured for seven days at 26(degrees)C to obtain L3s. The genus-specific FECRT was based on larval identification, integrating coproculture and FEC results. Larvae were identified as Haemonchus, Trichostrongylus, Teladorsagia, Oesophagostomum / Chabertia and Bunostomum. Molecular assays (a multiplex real-time PCR and two end-point PCRs) were also implemented on pooled samples to support the morphological identification. The Spearmann Rho test confirmed a high correlation between the two approaches (Rho = 0.941 and Rho = 0.914 respectively for Haemonchus and Trichostrongylus, the two most common genera). Both oxfendazole and eprinomectin were effective in one farm, while none in the other farm (FECR = 75.9% and 73.3% respectively). In the second farm, the genus-specific FECRT highlighted a different response to treatment among genera: oxfendazole lacked efficacy against both Haemonchus and Trichostrongylus spp., eprinomectin only against Haemonchus, while all other genera were susceptible to both drugs. This study brings new attention on the importance of adopting a genus-specific approach to identify and quantify differences in susceptibility to anthelmintics among genera in goats, providing support for FECRT interpretation, anthelmintic resistance evaluation and evidence-based GIN control.
ER  -

MAURIZIO, Anna; Lucie ŠKORPÍKOVÁ; Jana ILGOVÁ; Cinzia TESSARIN; Giorgia DOTTO; Nikol RESLOVÁ; Jaroslav VADLEJCH; Erica MARCHIORI; di Regalbono Antonio FRANGIPANE; Martin KAŠNÝ a Rudi CASSINI. Faecal egg count reduction test in goats: Zooming in on the genus level. \textit{Veterinary Parasitology}. AMSTERDAM: Elsevier Science, 2024, roč.~327, April, s.~1-8. ISSN~0304-4017. Dostupné z: https://doi.org/10.1016/j.vetpar.2024.110146.

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