Decellularization of Pig Lung to Yield Three-Dimensional
Scaffold for Lung Tissue Engineering

C 2024

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

ČIMBOROVÁ, Katarína; Hana KOTASOVÁ; Vendula PELKOVÁ; Veronika SEDLÁKOVÁ; Aleš HAMPL et al.

Základní údaje

Originální název

Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering

Autoři

ČIMBOROVÁ, Katarína; Hana KOTASOVÁ; Vendula PELKOVÁ; Veronika SEDLÁKOVÁ a Aleš HAMPL

Vydání

2. vyd. New York, 3D Cell Culture. Methods in Molecular Biology, od s. 21-33, 13 s. vol 2764, 2024

Nakladatel

Humana

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/24:00136048

Organizační jednotka

Lékařská fakulta

ISBN

978-1-0716-3673-2

DOI

https://doi.org/10.1007/978-1-0716-3674-9_3

EID Scopus

2-s2.0-85185856479

Klíčová slova anglicky

Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 4. 2025 12:17, Mgr. Tereza Miškechová

Anotace

V originále

Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.

Návaznosti

MUNI/A/1398/2021, interní kód MU

Název: Zdroje pro tkáňové inženýrství 12 (Akronym: TissueEng 12)

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 12

NV16-31501A, projekt VaV

Název: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

Citovat

ČIMBOROVÁ, Katarína; Hana KOTASOVÁ; Vendula PELKOVÁ; Veronika SEDLÁKOVÁ a Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. 3D Cell Culture. Methods in Molecular Biology. 2. vyd. New York: Humana, 2024, s. 21-33. vol 2764. ISBN 978-1-0716-3673-2. Dostupné z: https://doi.org/10.1007/978-1-0716-3674-9_3.

@inbook{2403779,
   author = {Čimborová, Katarína and Kotasová, Hana and Pelková, Vendula and Sedláková, Veronika and Hampl, Aleš},
   address = {New York},
   booktitle = {3D Cell Culture. Methods in Molecular Biology},
   doi = {https://doi.org/10.1007/978-1-0716-3674-9_3},
   edition = {2.},
   editor = {Sumbalova Koledova, Z.},
   keywords = {Decellularized scaffolds; peptide hydrogels; Micropatterning; phenotypic evaluation; bone tissue modeling; Biofabrication; Bioprinting; Organoids},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York},
   isbn = {978-1-0716-3673-2},
   pages = {21-33},
   publisher = {Humana},
   title = {Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3},
   year = {2024}
}

TY  - CHAP
ID  - 2403779
AU  - Čimborová, Katarína - Kotasová, Hana - Pelková, Vendula - Sedláková, Veronika - Hampl, Aleš
PY  - 2024
TI  - Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
VL  - vol 2764
PB  - Humana
CY  - New York
SN  - 9781071636732
KW  - Decellularized scaffolds
KW  - peptide hydrogels
KW  - Micropatterning
KW  - phenotypic evaluation
KW  - bone tissue modeling
KW  - Biofabrication
KW  - Bioprinting
KW  - Organoids
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-3674-9_3
N2  - Lately, the need for three-dimensional (3D) cell culture has been recognized in order to closely mimic the organization of native tissues. Thus, 3D scaffolds started to be employed to facilitate the 3D cell organization and enable the artificial tissue formation for the emerging tissue engineering applications. 3D scaffolds can be prepared by various techniques, each with certain advantages and disadvantages. Decellularization is an easy method based on removal of cells from native tissue sample, yielding extracellular matrix (ECM) scaffold with preserved architecture and bioactivity. This chapter provides a detailed protocol for decellularization of pig lung and also some basic assays for evaluation of its effectivity, such as determination of DNA content and histological verification of the selected ECM components. Such decellularized scaffold can subsequently be used for various tissue engineering applications, for example, for recellularization with cells of interest, for natural ECM hydrogel preparation, or as a bioink for 3D bioprinting.
ER  -

ČIMBOROVÁ, Katarína; Hana KOTASOVÁ; Vendula PELKOVÁ; Veronika SEDLÁKOVÁ a Aleš HAMPL. Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering. Online. In Sumbalova Koledova, Z. \textit{3D Cell Culture. Methods in Molecular Biology}. 2. vyd. New York: Humana, 2024, s.~21-33. vol 2764. ISBN~978-1-0716-3673-2. Dostupné z: https://doi.org/10.1007/978-1-0716-3674-9\_{}3.

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