Truncated vitronectin with e-cadherin enables the xeno-free
derivation ofhuman embryonic stem cells

k 2024

Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL et. al.

Basic information

Original name

Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells

Authors

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL and Irena KOUTNÁ

Edition

23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy, 2024

Other information

Language

English

Type of outcome

Prezentace na konferencích

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

ISSN 2336-7210

Abstract

V originále

Human embryonic stem cells (hESCs) are able to differentiate into any cell type of a human body, have self-renewal abilities and very high proliferative activity1. These unique abilities enable their use in cell therapy, disease modelling, and drug development. The derivation of hESCs is usually performed using an animal or human cell feeder layer, which is undefined and potentially contagious, and because of these difficulties, there is a tendency to replace feeders with xeno-free defined substrates in recent years2.We used truncated vitronectin with E-cadherin as adefined xeno-free substrate for the derivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers (ectoderm, mesoderm, and endoderm)3. We confirmed for the first time that truncated vitronectin with E-cadherin is a defined xeno-free substrate that is suitable for the derivation of hESCs involved in cell therapies.

Links

MUNI/A/1301/2022, interní kód MU

Name: Zdroje pro tkáňové inženýrství 13

Investor: Masaryk University

MUNI/A/1598/2023, interní kód MU

Name: Zdroje pro tkáňové inženýrství 14

Investor: Masaryk University, Resources pro tissue engineering 14

MUNI/IGA/1332/2021, interní kód MU

Name: The impact of derivation substrate on differentiation of human embryonic stem cells for the cell therapy

Investor: Masaryk University

NU22-08-00629, research and development project

Name: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL and Irena KOUTNÁ. Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells. In 23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy. 2024.

@proceedings{2403977,
   author = {Souralová, Tereza and Hulínová, Daniela and Ventruba, Pavel and Ješeta, Michal and Hampl, Aleš and Koutná, Irena},
   booktitle = {23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy},
   language = {eng},
   title = {Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells},
   url = {http://www.ccsss.cz/index.php/ccsss/issue/view/43/77},
   year = {2024}
}

TY  - CONF
ID  - 2403977
AU  - Souralová, Tereza - Hulínová, Daniela - Ventruba, Pavel - Ješeta, Michal - Hampl, Aleš - Koutná, Irena
PY  - 2024
TI  - Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells
UR  - http://www.ccsss.cz/index.php/ccsss/issue/view/43/77
N2  - Human embryonic stem cells (hESCs) are able to differentiate into any cell type of a human body, have self-renewal abilities and very high proliferative activity1. These unique abilities enable their use in cell therapy, disease modelling, and drug development. The derivation of hESCs is usually performed using an animal or human cell feeder layer, which is undefined and potentially contagious, and because of these difficulties, there is a tendency to replace feeders with xeno-free defined substrates in recent years2.We used truncated vitronectin with E-cadherin as adefined xeno-free substrate for the derivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers (ectoderm, mesoderm, and endoderm)3. We confirmed for the first time that truncated vitronectin with E-cadherin is a defined xeno-free substrate that is suitable for the derivation of hESCs involved in cell therapies.
ER  -

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Pavel VENTRUBA, Michal JEŠETA, Aleš HAMPL and Irena KOUTNÁ. Truncated vitronectin with e-cadherin enables the xeno-free derivation ofhuman embryonic stem cells. In \textit{23rd Interdisciplinary Meeting of Young Biologists, Biochemists, and Chemists, Milovy}. 2024.

Detailed Information on Publication Record