Characterisation of the Arabidopsis thaliana telomerase TERT-TR
complex

J 2024

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex

ŠTEFANOVIE, Barbora; Leon Paul JENNER; Lucie BOZDĚCHOVÁ; Petr FAJKUS; Eva SÝKOROVÁ et al.

Základní údaje

Originální název

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex

Autoři

ŠTEFANOVIE, Barbora; Leon Paul JENNER; Lucie BOZDĚCHOVÁ ; Petr FAJKUS; Eva SÝKOROVÁ; Jiří FAJKUS a Jan PALEČEK

Vydání

Plant Molecular Biology, Dordrecht (Netherlands), Springer, 2024, 0167-4412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00136070

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

A.thaliana telomerasa; AtTERT; AtTR; Protein-RNA interakce; kvasinkový troj-hybridní systém

Klíčová slova anglicky

A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid

Štítky

143160, AtTERT, plant, rivok, telomere

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 8. 2024 13:49, prof. RNDr. Jiří Fajkus, CSc.

Anotace

V originále

Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

GX20-01331X, projekt VaV

Název: Biogeneze a evoluce telomerázy u rostlin

Investor: Grantová agentura ČR, Biogenesis and evolution of telomerase in plants

MUNI/R/1142/2021, interní kód MU

Název: Visualization of the 3D structure of chromatin

Investor: Masarykova univerzita, Visualization of the 3D structure of chromatin, CAREER RESTART

Přiložené soubory

PMB-Stefanovie24_Characterisation_of_the_Arabidopsis_thaliana_telomerase_TERT-TR_complex.pdf

Citovat

ŠTEFANOVIE, Barbora; Leon Paul JENNER; Lucie BOZDĚCHOVÁ; Petr FAJKUS; Eva SÝKOROVÁ; Jiří FAJKUS a Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. Plant Molecular Biology. Dordrecht (Netherlands): Springer, 2024, roč. 114, č. 3, s. 1-12. ISSN 0167-4412. Dostupné z: https://doi.org/10.1007/s11103-024-01461-w.

@article{2405278,
   author = {Štefanovie, Barbora and Jenner, Leon Paul and Bozděchová, Lucie and Fajkus, Petr and Sýkorová, Eva and Fajkus, Jiří and Paleček, Jan},
   article_location = {Dordrecht (Netherlands)},
   article_number = {3},
   doi = {https://doi.org/10.1007/s11103-024-01461-w},
   keywords = {A.thaliana telomerase; AtTERT; AtTR; Protein-RNA interactions; Yeast three-hybrid},
   language = {eng},
   issn = {0167-4412},
   journal = {Plant Molecular Biology},
   title = {Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex},
   url = {https://link.springer.com/article/10.1007/s11103-024-01461-w},
   volume = {114},
   year = {2024}
}

TY  - JOUR
ID  - 2405278
AU  - Štefanovie, Barbora - Jenner, Leon Paul - Bozděchová, Lucie - Fajkus, Petr - Sýkorová, Eva - Fajkus, Jiří - Paleček, Jan
PY  - 2024
TI  - Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex
JF  - Plant Molecular Biology
VL  - 114
IS  - 3
SP  - 1-12
EP  - 1-12
PB  - Springer
SN  - 01674412
KW  - A.thaliana telomerase
KW  - AtTERT
KW  - AtTR
KW  - Protein-RNA interactions
KW  - Yeast three-hybrid
UR  - https://link.springer.com/article/10.1007/s11103-024-01461-w
N2  - Most eukaryotic organisms employ a telomerase complex for the maintenance of chromosome ends. The core of this complex is composed of telomerase reverse transcriptase (TERT) and telomerase RNA (TR) subunits. The TERT reverse transcriptase (RT) domain synthesises telomeric DNA using the TR template sequence. The other TERT domains contribute to this process in different ways. In particular, the TERT RNA-binding domain (TRBD) interacts with specific TR motif(s). Using a yeast 3-hybrid system, we show the critical role of Arabidopsis thaliana (At) TRBD and embryophyta-conserved KRxR motif in the unstructured linker preceding the TRBD domain for binding to the recently identified AtTR subunit. We also show the essential role of the predicted P4 stem and pseudoknot AtTR structures and provide evidence for the binding of AtTRBD to pseudoknot and KRxR motif stabilising interaction with the P4 stem structure. Our results thus provide the first insight into the core part of the plant telomerase complex.
ER  -

ŠTEFANOVIE, Barbora; Leon Paul JENNER; Lucie BOZDĚCHOVÁ; Petr FAJKUS; Eva SÝKOROVÁ; Jiří FAJKUS a Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. \textit{Plant Molecular Biology}. Dordrecht (Netherlands): Springer, 2024, roč.~114, č.~3, s.~1-12. ISSN~0167-4412. Dostupné z: https://doi.org/10.1007/s11103-024-01461-w.

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