| KRAMÁREK, Michal, Přemysl SOUČEK, Kamila RÉBLOVÁ, Lucie KAJAN GRODECKA and Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. Nucleic acids research. Oxford: Oxford University Press, 2024, vol. 52, No 10, p. 5959-5974. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae147. |
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@article{2407804,
author = {Kramárek, Michal and Souček, Přemysl and Réblová, Kamila and Kajan Grodecka, Lucie and Freiberger, Tomáš},
article_location = {Oxford},
article_number = {10},
doi = {http://dx.doi.org/10.1093/nar/gkae147},
keywords = {STAT3; snRNA},
language = {eng},
issn = {0305-1048},
journal = {Nucleic acids research},
title = {Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs},
url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true},
volume = {52},
year = {2024}
}
TY - JOUR
ID - 2407804
AU - Kramárek, Michal - Souček, Přemysl - Réblová, Kamila - Kajan Grodecka, Lucie - Freiberger, Tomáš
PY - 2024
TI - Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
JF - Nucleic acids research
VL - 52
IS - 10
SP - 5959-5974
EP - 5959-5974
PB - Oxford University Press
SN - 03051048
KW - STAT3
KW - snRNA
UR - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true
N2 - Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought.
ER -
KRAMÁREK, Michal, Přemysl SOUČEK, Kamila RÉBLOVÁ, Lucie KAJAN GRODECKA and Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2024, vol.~52, No~10, p.~5959-5974. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae147.
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