Concurrent D-loop cleavage by Mus81 and Yen1 yields
half-crossover precursors

J 2024

Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

CARREIRA, Raquel; Tomas LAMA-DIAZ; Maria CRUGEIRAS; F Javier AGUADO; Marek ŠEBESTA et. al.

Základní údaje

Originální název

Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors

Autoři

CARREIRA, Raquel; Tomas LAMA-DIAZ; Maria CRUGEIRAS; F Javier AGUADO; Marek ŠEBESTA (703 Slovensko, domácí); Lumír KREJČÍ (203 Česká republika, domácí) a Miguel G BLANCO

Vydání

Nucleic Acids Research, OXFORD, OXFORD UNIV PRESS, 2024, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 13.100

Kód RIV

RIV/00216224:14110/24:00139440

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1093/nar/gkae453

UT WoS

001237859900001

EID Scopus

2-s2.0-85198110669

Klíčová slova anglicky

D-loop; Mus81; Yen1; crossover

Štítky

14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2025 13:37, Mgr. Tereza Miškechová

Anotace

V originále

Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.

Návaznosti

GX21-22593X, projekt VaV

Název: Identifikace a charakterizace proteinů zahrnutých v metabolismu G-kvadruplexů a R-smyček a jejich vztah k replikaci DNA

206292/E/17/Z, interní kód MU

Název: Mechanics and execution of homologous recombination - biophysics to the organism

Investor: Wellcome Trust, Mechanics and execution of homologous recombination - biophysics to the organism

Citovat

CARREIRA, Raquel; Tomas LAMA-DIAZ; Maria CRUGEIRAS; F Javier AGUADO; Marek ŠEBESTA; Lumír KREJČÍ a Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. Nucleic Acids Research. OXFORD: OXFORD UNIV PRESS, 2024, roč. 52, č. 12, s. 7012-7030. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkae453.

@article{2407841,
   author = {Carreira, Raquel and LamaandDiaz, Tomas and Crugeiras, Maria and Aguado, F Javier and Šebesta, Marek and Krejčí, Lumír and Blanco, Miguel G},
   article_location = {OXFORD},
   article_number = {12},
   doi = {https://doi.org/10.1093/nar/gkae453},
   keywords = {D-loop; Mus81; Yen1; crossover},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors},
   url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true},
   volume = {52},
   year = {2024}
}

TY  - JOUR
ID  - 2407841
AU  - Carreira, Raquel - Lama-Diaz, Tomas - Crugeiras, Maria - Aguado, F Javier - Šebesta, Marek - Krejčí, Lumír - Blanco, Miguel G
PY  - 2024
TI  - Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
JF  - Nucleic Acids Research
VL  - 52
IS  - 12
SP  - 7012-7030
EP  - 7012-7030
PB  - OXFORD UNIV PRESS
SN  - 03051048
KW  - D-loop
KW  - Mus81
KW  - Yen1
KW  - crossover
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true
N2  - Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
ER  -

CARREIRA, Raquel; Tomas LAMA-DIAZ; Maria CRUGEIRAS; F Javier AGUADO; Marek ŠEBESTA; Lumír KREJČÍ a Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. \textit{Nucleic Acids Research}. OXFORD: OXFORD UNIV PRESS, 2024, roč.~52, č.~12, s.~7012-7030. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkae453.

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