Characterization of Aspergillus fumigatus secretome during
sublethal infection of Galleria mellonella larvae

J 2024

Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae

CURTIS, Aaron, Pavel DOBEŠ, Jacek MARCINIAK, Jana HURYCHOVÁ, Pavel HYRŠL et. al.

Základní údaje

Originální název

Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae

Autoři

CURTIS, Aaron, Pavel DOBEŠ (203 Česká republika, domácí), Jacek MARCINIAK (203 Česká republika, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí) a Kevin KAVANAGH

Vydání

Journal of Medical Microbiology, Microbiology Society, 2024, 0022-2615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.000 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/jmm.0.001844

UT WoS

001292084000007

Klíčová slova anglicky

Aspergillus; fungal–host interactions; Galleria mellonella; gliotoxin; proteomics

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 9. 2024 09:53, Mgr. Marie Šípková, DiS.

Anotace

V originále

Introduction. The fungal pathogen Aspergillus fumigatus can induce prolonged colonization of the lungs of susceptible patients, resulting in conditions such as allergic bronchopulmonary aspergillosis and chronic pulmonary aspergillosis. Hypothesis. Analysis of the A. fumigatus secretome released during sub-lethal infection of G. mellonella larvae may give an insight into products released during prolonged human colonisation. Methodology. Galleria mellonella larvae were infected with A. fumigatus, and the metabolism of host carbohydrate and proteins and production of fungal virulence factors were analysed. Label-free qualitative proteomic analysis was performed to identify fungal proteins in larvae at 96 hours post-infection and also to identify changes in the Galleria proteome as a result of infection. Results. Infected larvae demonstrated increasing concentrations of gliotoxin and siderophore and displayed reduced amounts of haemolymph carbohydrate and protein. Fungal proteins (399) were detected by qualitative proteomic analysis in cell-free haemolymph at 96 hours and could be categorized into seven groups, including virulence (n = 25), stress response (n = 34), DNA repair and replication (n = 39), translation (n = 22), metabolism (n = 42), released intracellular (n = 28) and cellular development and cell cycle (n = 53). Analysis of the Gallerial proteome at 96 hours post-infection revealed changes in the abundance of proteins associated with immune function, metabolism, cellular structure, insect development, transcription/translation and detoxification. Conclusion. Characterizing the impact of the fungal secretome on the host may provide an insight into how A. fumigatus damages tissue and suppresses the immune response during long-term pulmonary colonization.

Citovat

CURTIS, Aaron, Pavel DOBEŠ, Jacek MARCINIAK, Jana HURYCHOVÁ, Pavel HYRŠL a Kevin KAVANAGH. Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae. Journal of Medical Microbiology. Microbiology Society, 2024, roč. 73, č. 6, s. 1-11. ISSN 0022-2615. Dostupné z: https://dx.doi.org/10.1099/jmm.0.001844.

@article{2408277,
   author = {Curtis, Aaron and Dobeš, Pavel and Marciniak, Jacek and Hurychová, Jana and Hyršl, Pavel and Kavanagh, Kevin},
   article_number = {6},
   doi = {http://dx.doi.org/10.1099/jmm.0.001844},
   keywords = {Aspergillus; fungal–host interactions; Galleria mellonella; gliotoxin; proteomics},
   language = {eng},
   issn = {0022-2615},
   journal = {Journal of Medical Microbiology},
   title = {Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae},
   url = {https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001844},
   volume = {73},
   year = {2024}
}

TY  - JOUR
ID  - 2408277
AU  - Curtis, Aaron - Dobeš, Pavel - Marciniak, Jacek - Hurychová, Jana - Hyršl, Pavel - Kavanagh, Kevin
PY  - 2024
TI  - Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae
JF  - Journal of Medical Microbiology
VL  - 73
IS  - 6
SP  - 1-11
EP  - 1-11
PB  - Microbiology Society
SN  - 00222615
KW  - Aspergillus
KW  - fungal–host interactions
KW  - Galleria mellonella
KW  - gliotoxin
KW  - proteomics
UR  - https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001844
N2  - Introduction. The fungal pathogen Aspergillus fumigatus can induce prolonged colonization of the lungs of susceptible patients, resulting in conditions such as allergic bronchopulmonary aspergillosis and chronic pulmonary aspergillosis. Hypothesis. Analysis of the A. fumigatus secretome released during sub-lethal infection of G. mellonella larvae may give an insight into products released during prolonged human colonisation. Methodology. Galleria mellonella larvae were infected with A. fumigatus, and the metabolism of host carbohydrate and proteins and production of fungal virulence factors were analysed. Label-free qualitative proteomic analysis was performed to identify fungal proteins in larvae at 96 hours post-infection and also to identify changes in the Galleria proteome as a result of infection. Results. Infected larvae demonstrated increasing concentrations of gliotoxin and siderophore and displayed reduced amounts of haemolymph carbohydrate and protein. Fungal proteins (399) were detected by qualitative proteomic analysis in cell-free haemolymph at 96 hours and could be categorized into seven groups, including virulence (n = 25), stress response (n = 34), DNA repair and replication (n = 39), translation (n = 22), metabolism (n = 42), released intracellular (n = 28) and cellular development and cell cycle (n = 53). Analysis of the Gallerial proteome at 96 hours post-infection revealed changes in the abundance of proteins associated with immune function, metabolism, cellular structure, insect development, transcription/translation and detoxification. Conclusion. Characterizing the impact of the fungal secretome on the host may provide an insight into how A. fumigatus damages tissue and suppresses the immune response during long-term pulmonary colonization.
ER  -

CURTIS, Aaron, Pavel DOBEŠ, Jacek MARCINIAK, Jana HURYCHOVÁ, Pavel HYRŠL a Kevin KAVANAGH. Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae. \textit{Journal of Medical Microbiology}. Microbiology Society, 2024, roč.~73, č.~6, s.~1-11. ISSN~0022-2615. Dostupné z: https://dx.doi.org/10.1099/jmm.0.001844.

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