J 2019

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level

PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA et. al.

Základní údaje

Originální název

Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level

Autoři

PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA, Jan KOLEK, Pavlina KOSCOVA a Ivo PROVAZNIK

Vydání

Nature Scientific Reports, London, NATURE RESEARCH, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10700 1.7 Other natural sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 3.998

DOI

UT WoS

000457616300259

Klíčová slova anglicky

ACETONE-BUTANOL FERMENTATION; CYCLOPROPANE FATTY-ACIDS; FACTOR HOMOLOGOUS GENES; BACILLUS-SUBTILIS; SOLVENT PRODUCTION; ESCHERICHIA-COLI; DIFFERENTIAL EXPRESSION; GROWTH TEMPERATURE; HISTIDINE KINASES; DIVISION SITE

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 15. 10. 2024 09:07, Ing. Martina Blahová

Anotace

V originále

Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium.