Detailed Information on Publication Record
2019
Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq
KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA et. al.Basic information
Original name
Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq
Authors
KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA, Patricia MERTINKOVA, Monika DRAZOVSKA, Punit TYAGI and Mangesh BHIDE
Edition
Nature Scientific Reports, London, NATURE RESEARCH, 2019, 2045-2322
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.998
UT WoS
000502009000001
Keywords in English
MEMBRANE VESICLE VACCINE; IV PILI; DEATH RECEPTORS; INFECTION; INVASION; ACTIVATION; CROSS; RECRUITMENT; GONORRHOEA; EXPRESSION
Tags
International impact, Reviewed
Změněno: 15/10/2024 09:08, Ing. Martina Blahová
Abstract
V originále
Interaction of Neisseria meningitidis (NM) with human brain microvascular endothelial cells (hBMECs) initiates of multiple cellular processes, which allow bacterial translocation across the blood-brain barrier (BBB). NM is equipped with several antigens, which interacts with the host cell receptors. Recently we have shown that adhesin MafA (UniProtKB-X5EG71), relatively less studied protein, is one of those surface exposed antigens that adhere to hBMECs. The present study was designed to comprehensively map the undergoing biological processes in hBMECs challenged with NM or MafA using RNA sequencing. 708 and 726 differentially expressed genes (DEGs) were identified in hBMECs exposed to NM and MafA, respectively. Gene ontology analysis of the DEGs revealed that several biological processes, which may alter the permeability of BBB, were activated. Comparative analysis of DEGs revealed that MafA, alike NM, might provoke TLR-dependent pathway and augment cytokine response. Moreover, both MafA and NM were able to induce genes involved in cell surface modifications, endocytosis, extracellular matrix remodulation and anoikis/apoptosis. In conclusion, this study for the first time describes effect of NM on the global gene expression in hBMECs using high-throughput RNA-seq. It also presents ability of MafA to induce gene expression, which might aid NM in breaching the BBB.