Atypical applications of transverse diffusion of laminar flow
profiles methodology for in-capillary reactions in capillary
electrophoresis

J 2024

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

BRŽEZICKÁ, Taťána; Lenka KOHÚTOVÁ a Zdeněk GLATZ

Základní údaje

Originální název

Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis

Autoři

BRŽEZICKÁ, Taťána; Lenka KOHÚTOVÁ a Zdeněk GLATZ

Vydání

JOURNAL OF SEPARATION SCIENCE, GERMANY, WILEY-V C H VERLAG GMBH, 2024, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/24:00136352

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Capillary electrophoresis; In-capillary reaction; High-throughput screening technology; Transverse diffusion of laminar flow profiles methodology; Drug

Štítky

14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2025 07:34, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.

Návaznosti

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

MUNI/A/1582/2023, interní kód MU

Název: Podpora biochemického výzkumu v roce 2024

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2024

NU23-08-00229, projekt VaV

Název: Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie

Investor: Ministerstvo zdravotnictví ČR, Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie, Podprogram 1 - standardní

Citovat

BRŽEZICKÁ, Taťána; Lenka KOHÚTOVÁ a Zdeněk GLATZ. Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis. JOURNAL OF SEPARATION SCIENCE. GERMANY: WILEY-V C H VERLAG GMBH, 2024, roč. 47, č. 13, s. 1-24. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.202400157.

@article{2415977,
   author = {Bržezická, Taťána and Kohútová, Lenka and Glatz, Zdeněk},
   article_location = {GERMANY},
   article_number = {13},
   doi = {https://doi.org/10.1002/jssc.202400157},
   keywords = {Capillary electrophoresis; In-capillary reaction; High-throughput screening technology; Transverse diffusion of laminar flow profiles methodology; Drug},
   language = {eng},
   issn = {1615-9306},
   journal = {JOURNAL OF SEPARATION SCIENCE},
   title = {Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157},
   volume = {47},
   year = {2024}
}

TY  - JOUR
ID  - 2415977
AU  - Bržezická, Taťána - Kohútová, Lenka - Glatz, Zdeněk
PY  - 2024
TI  - Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis
JF  - JOURNAL OF SEPARATION SCIENCE
VL  - 47
IS  - 13
SP  - 1-24
EP  - 1-24
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - Capillary electrophoresis
KW  - In-capillary reaction
KW  - High-throughput screening technology
KW  - Transverse diffusion of laminar flow profiles methodology
KW  - Drug
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400157
N2  - CE is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterised by many well-known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked. It is the possibility of performing chemical and biochemical reactions at the nL scale inside the separation capillary. There are two basic formats applicable for this purpose, heterogeneous and homogeneous. In the former, one reactant is immobilised onto a particle or monolithic support or directly on the capillary wall, and the other is injected. In the latter, the reactant mixing inside a capillary is based on electromigration or diffusion. One of the diffusion-based methodologies, termed Transverse Diffusion of Laminar Flow Profiles, is the subject of this review. Since most studies utilising in-capillary reactions in CE focus on enzymes, which are being continuously and exhaustively reviewed, this review covers the atypical applications of this methodology, but still in the bioanalytical field. As can be seen from the demonstrated applications, they are not limited to reactions, but can also be utilised for other biochemical systems such as affinity interaction studies or biochemically important processes such as cell lysis, even in single-cell format.
ER  -

BRŽEZICKÁ, Taťána; Lenka KOHÚTOVÁ a Zdeněk GLATZ. Atypical applications of transverse diffusion of laminar flow profiles methodology for in-capillary reactions in capillary electrophoresis. \textit{JOURNAL OF SEPARATION SCIENCE}. GERMANY: WILEY-V C H VERLAG GMBH, 2024, roč.~47, č.~13, s.~1-24. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.202400157.

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