Developmental regulation of the maize Zm-p60.1 gene encoding a
beta-glucosidase located to plastids

J 2000

Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN et. al.

Základní údaje

Originální název

Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids

Autoři

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN a Klaus PALME

Vydání

Planta, Springer Berlin Heidelberg 2000, 2000, 0032-0935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.199

Kód RIV

RIV/00216224:14310/00:00001867

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea

Změněno: 7. 12. 2001 16:08, prof. RNDr. Břetislav Brzobohatý, CSc.

Anotace

V originále

A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicroscopy, the Zm-p60.1 protein was located exclusively in the plastids.

Návaznosti

VS96096, projekt VaV

Název: Laboratoř molekulární fyziologie rostlin

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Laboratoř molekulární fyziologie rostlin

Citovat

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN a Klaus PALME. Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids. Planta. Springer Berlin Heidelberg 2000, 2000, roč. 210, č. 3, s. 407-415. ISSN 0032-0935.

@article{242591,
   author = {Kristoffersen, Peter and Brzobohatý, Břetislav and Höhfeld, Ingo and Bako, Laszlo and Melkonian, Michael and Palme, Klaus},
   article_number = {3},
   keywords = {cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea},
   language = {eng},
   issn = {0032-0935},
   journal = {Planta},
   title = {Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids},
   volume = {210},
   year = {2000}
}

TY  - JOUR
ID  - 242591
AU  - Kristoffersen, Peter - Brzobohatý, Břetislav - Höhfeld, Ingo - Bako, Laszlo - Melkonian, Michael - Palme, Klaus
PY  - 2000
TI  - Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids
JF  - Planta
VL  - 210
IS  - 3
SP  - 407
EP  - 407
PB  - Springer Berlin Heidelberg 2000
SN  - 00320935
KW  - cytokinin - Cytokinin conjugate - -Glucosidase - Immunolocalization - Zea
N2  - A beta-glucosidase that cleaves the biologically inactive hormone conjugates cytokinin-O- and kinetin-N3-glucosides is encoded by the maize Zm-p60.1 gene. The expression of the Zm-p60.1 gene was analyzed by Northern blot analysis and in-situ hybridization. It was found that the expression levels of the Zm-p60.1-specific mRNA changed after pollination of carpellate inflorescences. The Zm-p60.1 c DNA was expressed in E. coli and antibodies were raised against the protein. An antibody was used to determine the tissue-specific localization of this protein. By in situ immunolocalization experiments, this protein was found to be located in cell layers below the epidermis and around the vascular bundles of the coleoptile. In the primary leaf, the Zm-p60.1 protein was detected in cells of the outermost cell layer and arround the vascular tissue. In floral tissue, Zm-p60.1 was present in the glumes, the carpels and in the outer cell layer of the style. In the coleoptiles, as determined by immuno-electronmicroscopy, the Zm-p60.1 protein was located exclusively in the plastids.
ER  -

KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN a Klaus PALME. Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids. \textit{Planta}. Springer Berlin Heidelberg 2000, 2000, roč.~210, č.~3, s.~407-415. ISSN~0032-0935.

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