Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland

C 2024

Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland

KUONY, Alison; Matea BREZAK; René-Marc MÈGE a Zuzana SUMBALOVÁ KOLEDOVÁ

Základní údaje

Originální název

Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland

Autoři

KUONY, Alison; Matea BREZAK ; René-Marc MÈGE a Zuzana SUMBALOVÁ KOLEDOVÁ

Vydání

2. vyd. New York, 3D Cell Culture: Methods in Molecular Biology, od s. 145-156, 11 s. volume 2764, 2024

Nakladatel

Humana New York

Další údaje

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISBN

978-1-0716-3673-2

DOI

https://doi.org/10.1007/978-1-0716-3674-9_10

EID Scopus

2-s2.0-85185860421

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 3. 2026 16:20, Mgr. Petra Trembecká, Ph.D.

Anotace

V originále

Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands.

Citovat

KUONY, Alison; Matea BREZAK; René-Marc MÈGE a Zuzana SUMBALOVÁ KOLEDOVÁ. Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland. In Zuzana Sumbalova Koledova. 3D Cell Culture: Methods in Molecular Biology. 2. vyd. New York: Humana New York, 2024, s. 145-156, 11 s. volume 2764. ISBN 978-1-0716-3673-2. Dostupné z: https://doi.org/10.1007/978-1-0716-3674-9_10.

@inbook{2431278,
   author = {Kuony, Alison and Brezak, Matea and Mège, RenéandMarc and Sumbalová Koledová, Zuzana},
   address = {New York},
   booktitle = {3D Cell Culture: Methods in Molecular Biology},
   doi = {https://doi.org/10.1007/978-1-0716-3674-9_10},
   edition = {2},
   editor = {Zuzana Sumbalova Koledova},
   location = {New York},
   isbn = {978-1-0716-3673-2},
   pages = {145-156},
   publisher = {Humana New York},
   title = {Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland},
   url = {https://doi.org/10.1007/978-1-0716-3674-9_10},
   year = {2024}
}

TY  - CHAP
ID  - 2431278
AU  - Kuony, Alison - Brezak, Matea - Mège, René-Marc - Sumbalová Koledová, Zuzana
PY  - 2024
TI  - Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland
VL  - volume 2764
PB  - Humana New York
CY  - New York
SN  - 9781071636732
UR  - https://doi.org/10.1007/978-1-0716-3674-9_10
N2  - Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands.
ER  -

KUONY, Alison; Matea BREZAK; René-Marc MÈGE a Zuzana SUMBALOVÁ KOLEDOVÁ. Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland. In Zuzana Sumbalova Koledova. \textit{3D Cell Culture: Methods in Molecular Biology}. 2. vyd. New York: Humana New York, 2024, s.~145-156, 11 s. volume 2764. ISBN~978-1-0716-3673-2. Dostupné z: https://doi.org/10.1007/978-1-0716-3674-9\_{}10.

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