High-throughput sequencing of Strongyloides stercoralis – a
fatal disseminated infection in a dog

J 2024

High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog

NOSKOVÁ, Eva; Vlasta SVOBODOVÁ; Vilma HYPSKÁ; Argiñe CEREZO-ECHEVARRIA; Terézia KURUCOVÁ et. al.

Základní údaje

Originální název

High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog

Autoři

NOSKOVÁ, Eva; Vlasta SVOBODOVÁ; Vilma HYPSKÁ; Argiñe CEREZO-ECHEVARRIA; Terézia KURUCOVÁ ; Vladislav ILÍK; David MODRÝ a Barbora PAFČO

Vydání

Parasitology, Cambridge University Press, 2024, 0031-1820

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30310 Parasitology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.400

Kód RIV

RIV/00216224:14310/24:00137128

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1017/S0031182024000568

UT WoS

001232015200001

EID Scopus

2-s2.0-85192692601

Klíčová slova anglicky

dog; high-throughput sequencing; Strongyloides stercoralis; Strongyloidiasis; zoonotic

Štítky

14314020, CF GEN, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 3. 2025 10:20, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The rhabditid nematode Strongyloides stercoralis is known worldwide as the causative agent of strongyloidiasis in humans. In addition to public health concerns, S. stercoralis also infects dogs, which represent a possible reservoir for potentially zoonotic transmissions. We describe the first confirmed case of fatal disseminated infection in a dog in the Czech Republic. The microscopic and histological results were supported by a complex genotyping approach. Using high-throughput sequencing of the hypervariable region (HVR-IV) of 18S rDNA and Sanger sequencing of the partial cytochrome c oxidase subunit 1 gene (cox1), the potentially zoonotic haplotype/lineage A of S. stercoralis was confirmed, while the solely canine haplotype/lineage B was not found. The development of the disease is mainly associated with immunodeficiency, and in this case, it was triggered by inappropriate treatment, in particular the use of corticosteroids.

Návaznosti

MUNI/A/1602/2023, interní kód MU

Název: Evoluční a ekologické procesy v modelových parazito-hostitelských systémech a vodních společenstvech

Investor: Masarykova univerzita, Evoluční a ekologické procesy v modelových parazito-hostitelských systémech a vodních společenstvech

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

90267, velká výzkumná infrastruktura

Název: NCMG III

Citovat

NOSKOVÁ, Eva; Vlasta SVOBODOVÁ; Vilma HYPSKÁ; Argiñe CEREZO-ECHEVARRIA; Terézia KURUCOVÁ; Vladislav ILÍK; David MODRÝ a Barbora PAFČO. High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog. Parasitology. Cambridge University Press, 2024, roč. 151, č. 6, s. 587-593. ISSN 0031-1820. Dostupné z: https://doi.org/10.1017/S0031182024000568.

@article{2433499,
   author = {Nosková, Eva and Svobodová, Vlasta and Hypská, Vilma and CerezoandEchevarria, Argiñe and Kurucová, Terézia and Ilík, Vladislav and Modrý, David and Pafčo, Barbora},
   article_number = {6},
   doi = {https://doi.org/10.1017/S0031182024000568},
   keywords = {dog; high-throughput sequencing; Strongyloides stercoralis; Strongyloidiasis; zoonotic},
   language = {eng},
   issn = {0031-1820},
   journal = {Parasitology},
   title = {High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog},
   url = {https://www.cambridge.org/core/journals/parasitology/article/highthroughput-sequencing-of-strongyloides-stercoralis-a-fatal-disseminated-infection-in-a-dog/E085E2C44E784F4EEA6DAEFF5E23199B#article},
   volume = {151},
   year = {2024}
}

TY  - JOUR
ID  - 2433499
AU  - Nosková, Eva - Svobodová, Vlasta - Hypská, Vilma - Cerezo-Echevarria, Argiñe - Kurucová, Terézia - Ilík, Vladislav - Modrý, David - Pafčo, Barbora
PY  - 2024
TI  - High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog
JF  - Parasitology
VL  - 151
IS  - 6
SP  - 587-593
EP  - 587-593
PB  - Cambridge University Press
SN  - 00311820
KW  - dog
KW  - high-throughput sequencing
KW  - Strongyloides stercoralis
KW  - Strongyloidiasis
KW  - zoonotic
UR  - https://www.cambridge.org/core/journals/parasitology/article/highthroughput-sequencing-of-strongyloides-stercoralis-a-fatal-disseminated-infection-in-a-dog/E085E2C44E784F4EEA6DAEFF5E23199B#article
N2  - The rhabditid nematode Strongyloides stercoralis is known worldwide as the causative agent of strongyloidiasis in humans. In addition to public health concerns, S. stercoralis also infects dogs, which represent a possible reservoir for potentially zoonotic transmissions. We describe the first confirmed case of fatal disseminated infection in a dog in the Czech Republic. The microscopic and histological results were supported by a complex genotyping approach. Using high-throughput sequencing of the hypervariable region (HVR-IV) of 18S rDNA and Sanger sequencing of the partial cytochrome c oxidase subunit 1 gene (cox1), the potentially zoonotic haplotype/lineage A of S. stercoralis was confirmed, while the solely canine haplotype/lineage B was not found. The development of the disease is mainly associated with immunodeficiency, and in this case, it was triggered by inappropriate treatment, in particular the use of corticosteroids.
ER  -

NOSKOVÁ, Eva; Vlasta SVOBODOVÁ; Vilma HYPSKÁ; Argiñe CEREZO-ECHEVARRIA; Terézia KURUCOVÁ; Vladislav ILÍK; David MODRÝ a Barbora PAFČO. High-throughput sequencing of Strongyloides stercoralis – a fatal disseminated infection in a dog. \textit{Parasitology}. Cambridge University Press, 2024, roč.~151, č.~6, s.~587-593. ISSN~0031-1820. Dostupné z: https://doi.org/10.1017/S0031182024000568.

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