KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA
stability and telomere homeostasis

J 2024

KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis

ZÁVODNÍK, Michal, Veronika PAVLIŠTOVÁ, Adéla MACHELOVÁ, Martin LYČKA, Iva MOZGOVA et. al.

Základní údaje

Originální název

KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis

Autoři

ZÁVODNÍK, Michal, Veronika PAVLIŠTOVÁ, Adéla MACHELOVÁ, Martin LYČKA, Iva MOZGOVA, Karolína CAKLOVÁ, Martina DVOŘÁČKOVÁ a Jiří FAJKUS

Vydání

Plant Journal, Hoboken (USA), Wiley-Blackwell, 2024, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.200 v roce 2022

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/tpj.16718

UT WoS

001186107900001

Klíčová slova anglicky

Arabidopsis thaliana; CAF-1; FAS1; ALT; KU70; telomeres; 45S rDNA; genome instability

Štítky

CF CELLIM, CF PLANT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 9. 2024 11:29, Mgr. Eva Dubská

Anotace

V originále

Deficiency in chromatin assembly factor-1 (CAF-1) in plants through dysfunction of its components, FASCIATA1 and 2 (FAS1, FAS2), leads to the specific and progressive loss of rDNA and telomere repeats in plants. This loss is attributed to defective repair mechanisms for the increased DNA breaks encountered during replication, a consequence of impaired replication-dependent chromatin assembly. In this study, we explore the role of KU70 in these processes. Our findings reveal that, although the rDNA copy number is reduced in ku70 mutants when compared with wild-type plants, it is not markedly affected by diverse KU70 status in fas1 mutants. This is consistent with our previous characterisation of rDNA loss in fas mutants as a consequence part of the single-strand annealing pathway of homology-dependent repair. In stark contrast to rDNA, KU70 dysfunction fully suppresses the loss of telomeres in fas1 plants and converts telomeres to their elongated and heterogeneous state typical for ku70 plants. We conclude that the alternative telomere lengthening pathway, known to be activated in the absence of KU70, overrides progressive telomere loss due to CAF-1 dysfunction.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

GX20-01331X, projekt VaV

Název: Biogeneze a evoluce telomerázy u rostlin

Investor: Grantová agentura ČR, Biogenesis and evolution of telomerase in plants

MUNI/R/1364/2023, interní kód MU

Název: Mechanizmy opravy DNA u rostliny Arabidopsis thaliana

Investor: Masarykova univerzita, Mechanizmy opravy DNA u rostliny Arabidopsis thaliana, CAREER RESTART

Citovat

ZÁVODNÍK, Michal, Veronika PAVLIŠTOVÁ, Adéla MACHELOVÁ, Martin LYČKA, Iva MOZGOVA, Karolína CAKLOVÁ, Martina DVOŘÁČKOVÁ a Jiří FAJKUS. KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis. Plant Journal. Hoboken (USA): Wiley-Blackwell, 2024, roč. 118, č. 6, s. 1922-1936. ISSN 0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.16718.

@article{2433938,
   author = {Závodník, Michal and Pavlištová, Veronika and Machelová, Adéla and Lyčka, Martin and Mozgova, Iva and Caklová, Karolína and Dvořáčková, Martina and Fajkus, Jiří},
   article_location = {Hoboken (USA)},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/tpj.16718},
   keywords = {Arabidopsis thaliana; CAF-1; FAS1; ALT; KU70; telomeres; 45S rDNA; genome instability},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.16718},
   volume = {118},
   year = {2024}
}

TY  - JOUR
ID  - 2433938
AU  - Závodník, Michal - Pavlištová, Veronika - Machelová, Adéla - Lyčka, Martin - Mozgova, Iva - Caklová, Karolína - Dvořáčková, Martina - Fajkus, Jiří
PY  - 2024
TI  - KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis
JF  - Plant Journal
VL  - 118
IS  - 6
SP  - 1922-1936
EP  - 1922-1936
PB  - Wiley-Blackwell
SN  - 09607412
KW  - Arabidopsis thaliana
KW  - CAF-1
KW  - FAS1
KW  - ALT
KW  - KU70
KW  - telomeres
KW  - 45S rDNA
KW  - genome instability
UR  - https://onlinelibrary.wiley.com/doi/10.1111/tpj.16718
N2  - Deficiency in chromatin assembly factor-1 (CAF-1) in plants through dysfunction of its components, FASCIATA1 and 2 (FAS1, FAS2), leads to the specific and progressive loss of rDNA and telomere repeats in plants. This loss is attributed to defective repair mechanisms for the increased DNA breaks encountered during replication, a consequence of impaired replication-dependent chromatin assembly. In this study, we explore the role of KU70 in these processes. Our findings reveal that, although the rDNA copy number is reduced in ku70 mutants when compared with wild-type plants, it is not markedly affected by diverse KU70 status in fas1 mutants. This is consistent with our previous characterisation of rDNA loss in fas mutants as a consequence part of the single-strand annealing pathway of homology-dependent repair. In stark contrast to rDNA, KU70 dysfunction fully suppresses the loss of telomeres in fas1 plants and converts telomeres to their elongated and heterogeneous state typical for ku70 plants. We conclude that the alternative telomere lengthening pathway, known to be activated in the absence of KU70, overrides progressive telomere loss due to CAF-1 dysfunction.
ER  -

ZÁVODNÍK, Michal, Veronika PAVLIŠTOVÁ, Adéla MACHELOVÁ, Martin LYČKA, Iva MOZGOVA, Karolína CAKLOVÁ, Martina DVOŘÁČKOVÁ a Jiří FAJKUS. KU70 and CAF-1 in Arabidopsis: Divergent roles in rDNA stability and telomere homeostasis. \textit{Plant Journal}. Hoboken (USA): Wiley-Blackwell, 2024, roč.~118, č.~6, s.~1922-1936. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.16718.

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