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@inproceedings{243611,
author = {Glatz, Zdeněk and Mašlaňová, Hana},
address = {Saarbrucken, Germany},
booktitle = {Book of Abstract "13th International symposium on high performance capillary electrophoresis and related techniques HPCE 2000".},
keywords = {glutathione; on-column derivatization; MEKC},
language = {eng},
location = {Saarbrucken, Germany},
pages = {155-156},
publisher = {Universites des Saarlandes},
title = {Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide},
year = {2000}
}
TY - JOUR
ID - 243611
AU - Glatz, Zdeněk - Mašlaňová, Hana
PY - 2000
TI - Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide
PB - Universites des Saarlandes
CY - Saarbrucken, Germany
KW - glutathione
KW - on-column derivatization
KW - MEKC
N2 - A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.
ER -
GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide. In \textit{Book of Abstract ''13th International symposium on high performance capillary electrophoresis and related techniques HPCE 2000''.}. Saarbrucken, Germany: Universites des Saarlandes, 2000, s.~155-156.
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